| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pENTR/H1/TO |
| 质粒类型: | Gateway系统载体；入门载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | Human HI/TO |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3869 bp |
| 5' 测序引物及序列: | M13 Fwd |
| 3' 测序引物及序列: | M13 Rev |
| 载体标签: | -- |
| 载体抗性: | Kanamycin |
| 筛选标记: | Zeocin |
| 备注: |
Contains a hybrid promoter consisting of human H1 promoter and two tetracycline operator sequence for RNA Polymerase III-dependant,
regulated expression of the short hairpin RNA (shRNA).
|
| 产品目录号: | K4920-00 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
pENTR/H1/TO
价格：1800元
联系方式：I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pENTR/H1/TO 3869 bp DNA SYN 06-Mar-2014
DEFINITION pENTR/H1/TO
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3869
/organism="pENTR/H1/TO"
/mol_type="other DNA"
terminator complement(268..295)
/label="rrnB_T2_terminator"
terminator complement(427..470)
/label="rrnB_T1_terminator"
misc_feature complement(532..551)
/label="EBV_rev_primer"
terminator complement(518..637)
/label="SV40_PA_terminator"
misc_feature complement(772..1146)
/label="sh_ble"
gene complement(775..1146)
/label="bleo"
/gene="bleo"
promoter complement(1147..1214)
/label="EM7_promoter"
misc_feature complement(1328..1347)
/label="SV40pro_F_primer"
rep_origin complement(1332..1409)
/label="SV40_origin"
promoter complement(1308..1576)
/label="SV40_promoter"
misc_feature 1379..1594
/label="SV40_enhancer"
misc_feature 1574..1594
/label="pBABE_3_primer"
misc_feature 1633..1655
/label="M13_pUC_fwd_primer"
misc_feature 1648..1664
/label="M13_forward20_primer"
misc_feature complement(1680..1779)
/label="attL1"
misc_feature 1848..1867
/label="H1_primer"
misc_feature complement(1978..1994)
/label="pBluescriptSK_primer"
misc_feature 1994..2093
/label="attL2"
promoter complement(2111..2129)
/label="T7_promoter"
misc_feature complement(2130..2148)
/label="M13_reverse_primer"
CDS 2263..3072
/label="KanR2"
/gene="KanR2"
/note="ORF frame 1"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGK
PDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRV
GIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
gene 2263..3072
/label="KanR2"
/gene="KanR2"
rep_origin 3203..3822
/label="pBR322_origin"
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAATTCGCCC TTAGACATGA TAAGATACAT TGATGAGTTT
541 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT
601 ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTGGGGTGGG CGAAGAACTC
661 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA
721 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTAGCACGT GTCAGTCCTG
781 CTCCTCGGCC ACGAAGTGCA CGCAGTTGCC GGCCGGGTCG CGCAGGGCGA ACTCCCGCCC
841 CCACGGCTGC TCGCCGATCT CGGTCATGGC CGGCCCGGAG GCGTCCCGGA AGTTCGTGGA
901 CACGACCTCC GACCACTCGG CGTACAGCTC GTCCAGGCCG CGCACCCACA CCCAGGCCAG
961 GGTGTTGTCC GGCACCATCT GGTCCTGGAC CGCGCTGATG AACAGGGTCA CGTCGTCCCG
1021 GACCACACCG GCGAAGTCGT CCTCCACGAA GTCCCGGGAG AACCCGAGCC GGTCGGTCCA
1081 GAACTCGACC GCTCCGGCGA CGTCGCGCGC GGTGAGCACC GGAACGGCAC TGGTCAACTT
1141 GGCCATGGTT TAGTTCCTCA CCTTGTCGTA TTATACTATG CCGATATACT ATGCCGATGA
1201 TTAATTGTCA ACACGTGCTG ATCAGATCCG AAAATGGATA TACAAGCTCC CGGGAGCTTT
1261 TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCA
1321 GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG
1381 GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT
1441 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA
1501 CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT
1561 GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCC GAAGGGCGAA TTAACGCTAG
1621 CATGGATGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTCTTAAG CTCGGGCCCC
1681 AAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT
1741 GCTTTTTTAT AATGCCAACT TTGTACAAAA AAGCAGGCTT TAAAGGAACC AATTCAGTCG
1801 ACTGGATCCG GTACCGGGCC CCCCCTCGAA GATCTAATAT TTGCATGTCG CTATGTGTTC
1861 TGGGAAATCA CCATAAACGT GAAATCCCTA TCAGTGATAG AGACTTATAA GTTCCCTATC
1921 AGTGATAGAG ACACCTTTTT TGTCGAGCTT CCTTCGGGAA GCTCTCCATA TTTTTTGGAT
1981 CCACTAGTTC TAGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA
2041 TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT TTGCCATCCA
2101 GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG
2161 CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA
2221 ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG
2281 GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG
2341 GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT
2401 GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG
2461 ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC
2521 CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG
2581 GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG
2641 CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT
2701 CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC
2761 GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC
2821 TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG
2881 GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT
2941 CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT
3001 CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT
3061 GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT
3121 TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC
3181 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
3241 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
3301 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
3361 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT
3421 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
3481 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
3541 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
3601 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
3661 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
3721 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
3781 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
3841 GCCTTTTGCT GGCCTTTTGC TCACATGTT
//
载体质粒图谱pdf版和相关资料下载
载体应用举例
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