pcDNA3.1/nV5-DEST

价格：2000元

联系方式：I47-825O-882O

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pcDNA3.1/nV5-DEST载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pcDNA3.1/nV5-DEST
质粒类型:	Gateway载体；哺乳细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	CMV
克隆方法:	Gateway
载体大小:	7136bp
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	V5 Epitope Tag
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
克隆菌株:	ccdB阳性筛选 DB3.1 感受态细胞，阴性筛选用 OmniMAX2-T1 感受态细胞
备注:	切割位点：TEV蛋白酶识别位点
产品目录号:	12290-010
稳定性:	稳表达
组成型:	组成型
病毒/非病毒:	非病毒

pcDNA3.1/nV5-DEST质粒图谱载体图谱和pcDNA3.1/nV5-DEST载体序列质粒序列多克隆位点信息

pcDNA3.1-nV5-DEST载体图谱

pcDNA3.1/nV5-DEST质粒载体简介

Gateway哺乳动物表达载体选择指南：

pDEST12.2   不带标签

pDEST26     N-His标签 G418筛选

pDEST27   N-GST标签 G418筛选

pDEST40   C-His标签、C-V5标签   G418筛选

pcDNA-DEST40   C-His标签、C-V5标签   G418筛选

pcDNA-DEST47 C-绿色荧光蛋白 G418筛选

pcDNA-DEST53 N-绿色荧光蛋白 G418筛选

pcDNA3.2/V5-DEST   C-V5标签   G418筛选

pcDNA6.2/V5-DEST   C-V5标签 Blasticidin S筛选

pcDNA6.2/N-YFP-DEST   N-黄色荧光蛋白 Blasticidin S筛选

pcDNA6.2/N-EmGFP-DEST N-绿色荧光蛋白 Blasticidin S筛选

pcDNA6.2/C-YFP-DEST C-黄色荧光蛋白 Blasticidin S筛选

pcDNA6.2/C-EmGFP-DEST C-绿色荧光蛋白 Blasticidin S筛选

pcDNA6.2/BioEase-DEST N-生物素 Blasticidin S筛选

pcDNA3.1/nV5-DEST质粒序列载体序列

GACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTT
AAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACA
ACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCG
ATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTC
ATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG
CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC
ATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCC
AAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTA
TGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGC
AGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA
TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACG
CAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCA
CTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGTTAAGCTTACC
ATGGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGGAAAACCTGTATTTTCAGGGCCCGG
ATCCGTCGACGAATTCTGCAGATATCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATAT
AAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATC
CAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTG
GATTTTGAGTTAGGATCCGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGA
TATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAAT
GTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAA
GTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATG
AAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAA
CGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGT
GGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCC
AATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTT
TCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGC
CGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGC
GGGGCGTAAACGCGTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTT
GCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGT
ATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAA
GGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATA
TTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTC
CCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGT
GTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCA
TTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATA
GTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATT
GATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATAAACCCGCTGATCAGC
CTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAA
GGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATT
CTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGG
GGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCG
CCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCG
CCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGC
TCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGAT
TAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCA
CGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGA
TTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCG
AATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTAT
GCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGT
ATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAA
CTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGC
CGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAG
CTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTG
AACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACA
ACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTC
AAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGA
CGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGA
AGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCA
ATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGC
GAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGC
GCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGC
GATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGG
GTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATG
GGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTT
CTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATC
ACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGC
TGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTT
ATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAG
TTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTG
GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAG
CCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTC
ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGC
GGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAA
GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCAT
AGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTAC
CGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTC
AGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCAC
TGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTAC
GGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCG
CAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAAC
TCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAAT
GAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGA
GGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT
ACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTC
CAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGC
CTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAAC
GTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTT
CCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCC
GATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTT
ACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGT
GTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTT
AAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCC
AGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGT
GAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCAT
ACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAA
TGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC

pcDNA4/HisMax B	pMDLg/pRRE
pIJ702-del35-55	pLNCX2
pNEO	pSUMO
pEF6/myc-His B	pSET152
pLVX-DD-ZsGreen1 Control	pBD-NF-kB
pDEST26	pUG6
pRSET-EGFP	c-Flag pcDNA3
pRB373	pALEX a,b,c
pCGN978	pFLAG-CMV2
pdKeima-Red-S1	pATPase_TA
pMal-c4X	pUCTag
pCas9D10A-GFP	pNEO-ars
pBBR1MCS-2	pTi
pSP72	pAD-SV40T
pHSG422	pIC20H
pREP4	EWD299
pDC515	pBT-4
pET-28a-BFP-C	pOrf681_TA
pKLAC1	pcDNA3.1-GP130v1
pcDNA5/FRT	pJSC73
pPIC9k-His	pFLAG-CMV-3
pTet-Splice	pOM2
pBad43	pPIC9
pSC101	PX543
pSUPER.neo+gfp	pcDNA5/FRT/V5-His-TOPO
pBluescript II KS(-)	pTP-4
pET-32 EK/LIC	pCL-Ampho
pProLabel-C	pHS85
pCREj-2	pHB-1
pL-brevisGH3-MBP	pLLP-OmpA
pML21	pET-41a(+)
pHV23	pVPack-GP
pDsRed-Monomer	pEF1/V5-HisA
pET-12c(+)	pLexm
pCRE-DD-AmCyan1	pcDNA6.2-DsRed2-MCS1 miR
pWF1	pSC101-EcoRImeth+
pET-21a(+)	pAGO
pMal-c2G	pCRE-MetLuc2-Reporter
pMT-Bip-V5-HisA	pHY15
pPIC ZαGB	pAcGFP1-N2
pAN7-1	pCDH-CMV-MCS-EF1a-RFP+BSD
Pgex-HRV3C	pcDNA3.1(+)/CAT
pCH2	pUC4-KISS
pGL4.72[hRlucCP]	pSH65
pENTR11-Dual	pBAD18
pGL4.38[luc2P/p53 RE/Hygro]	pET-32a(+)
pMTD1.3xA	pGL4.41[luc2P/HSE/Hygro]
pGL4.45[luc2P/ISRE/Hygro]	pACT-MyoD
ISceI-GR-RFP	pCYM1
pIκB-EGFP	pFA1

pCS2TAL3-RR	pNEU-hMbPylRS-4xU6M15
pJKR-L-tetR	pCXLE-hOCT3/4
pMonAID_nCas9-PmCDA_Hyg_ALS	pJSC131 - Bacterial expression plasmid f
pLenti-DsRed_IRES_MAPT:EGFP	pAAVS1-PC-GCaMP6f
Lenti-luciferase-P2A-Neo	phage UbiC NLS HA stdMCP stdHalo
PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a	pEGFP-LC3
pAc-Neo-OVA	pLenti X2 Puro DEST (w16-1)
pTRKH2	CKS2 gRNA (BRDN0001149143)
pCAS9-mCherry empty	pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-
pEGFP-puro	1306 pSG5L HA FKHR wt
pAc-sgRNA-Cas9	pGGA008
BPK3082	pNeae2
pSR47	p201G Cas9
EC2_3_dCas9_Mxi1_sgRNA	pFC334
pET28a-At-TRL	pTRE Tight ArchT-GFP
pAC1404-pCR8-mRuby2_NLSPUFa	EC1000
pT3-Neo-EF1a-GFP	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)	pLentiV2-dCas9-VP64
pMJ329: pCMV-MmeFz2	pCAG-GBP1-10gly-lexADBD
pPPEM	pMal-T-Avi-His/BirA
ilux pGEX(-)	pRAD1
VVT1	TAL3393
c-myc-PT3EF1a	FUmGW
pSELECT-HA-mFOXO1	pMSCV-IRES-GFP II (pMIG II)
pCS2TAL3-DD	pT4-CMV-GFP
pM-Cas9	pcDNA3.4_7C11-1 heavy chain
AbVec1.1-IGKC	Tet-O-FUW-Neurod1
pGH335_MS2-AID*Δ-Hygro	3XMEF2-luc
LB1366 (Bcl-2 promoter with MHS1234)	NM9-SpCas9-NLS3
p58sheLL	pYES-mtGFP
pXAT2	pUCC001
pBHt2	pICH86966::AtU6p::sgRNA_PDS
pRC-CMV-Rev1b	pCW-Cas9
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV	pLVX-M-puro
pTEF:ATP	pCDH-puro-Bcl-XL
GST-HA-GFP11-N1	pAraGFPCDF
pNN9	Cbh_v5 AAV-CBE N-terminal
pMSCV-hygro-STING	pNK9
pM1s3AsR_TS-C7	pSBtet-GP
pLenti-EML4-ALK variant 3a	pHAGE-PTCH1
pAAV2/8	MSCV-(pBabe mcs)- human p210BCR-ABL-IRES
BECLIN-HA WT	pCMV-hA3G-BE3
pAAV-EFS-CjCas9-eGFP-HIF1a	pCMV-MMLVgag-3xNES-ABE8e
pGL3 2 kb prom. CD274	pQUAST>stop>mCD8-GFP
PB_tre_Cas9	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
pC003 - LshC2C2 locus into pACYC184 for	pAAVS1-PDi-CRISPRn
pLJC5-3XHA-EGFP-PEX26	pMXs-hOCT4 (Plath)
pAAV-EF1a-FLEX-GTB	LeGO-G
800 pSG5L HA PTEN wt	401 pSG5L
EC2_10_dCas9_VPR_HC_sgRNA	pDIRECT_23D

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pcDNA3.1/nV5-DEST

买家导航

pcDNA3.1/nV5-DEST载体质粒基本信息

pcDNA3.1/nV5-DEST质粒图谱载体图谱和pcDNA3.1/nV5-DEST载体序列质粒序列多克隆位点信息

pcDNA3.1/nV5-DEST质粒载体简介

pcDNA3.1/nV5-DEST质粒序列载体序列

pcDNA3.1/nV5-DEST载体图谱质粒图谱pdf版和pcDNA3.1/nV5-DEST载体序列质粒序列等相关资料下载

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