| 出品人: | Meng,J., Wang,H. |
|---|---|
| 载体名称: | pBBR1MCS-6 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 5859 bp |
| 5' 测序引物及序列: | M13 Forward 5’TGTAAAACGACGGCCAGT 3’ |
| 3' 测序引物及序列: | M13 Reverse 5’CAGGAAACAGCTATGAC 3’ |
| 载体标签: | -- |
| 载体抗性: | 链霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | 可用蓝白斑筛选法 |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBBR1MCS-6载体质粒基本信息
pBBR1MCS-6质粒图谱载体图谱和pBBR1MCS-6载体序列质粒序列多克隆位点信息
pBBR1MCS-6质粒载体简介
pBBR1MCS-6质粒序列载体序列
LOCUS KC698875 5859 bp DNA circular SYN 26-AUG-2013
DEFINITION Cloning vector pBBR1MCS-6, complete sequence.
ACCESSION KC698875
VERSION KC698875.1 GI:531997060
AUTHORS Meng,J., Wang,H., Liu,X., Lin,J., Pang,X. and Lin,J.
TITLE Construction of small plasmid vectors for use in genetic
improvement of the extremely acidophilic Acidithiobacillus caldus
JOURNAL Submitted (27-FEB-2013) State Key Laboratory of Microbial
Technology, Shandong University, Shanda Nanlu 27#, Jinan, Shandong
250100, China
FEATURES Location/Qualifiers
source 1..5859
/organism="Cloning vector pBBR1MCS-6"
/mol_type="other DNA"
/db_xref="taxon:1387373"
gene complement(join(5660..5859,1..799))
/gene="mob"
CDS complement(join(5660..5859,1..799))
/gene="mob"
/note="required for plasmid mobilization"
/codon_start=1
/transl_table=11
/product="Mob"
/protein_id="AGT62526.1"
/db_xref="GI:531997062"
/translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLG"
gene 1793..2455
/gene="rep"
CDS 1793..2455
/gene="rep"
/note="required for pBBR1 derivative plasmid replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AGT62525.1"
/db_xref="GI:531997061"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene complement(3013..3378)
/gene="lacZ"
CDS complement(3013..3378)
/gene="lacZ"
/note="beta-galactosidase; alpha peptide of LacZ"
/codon_start=1
/transl_table=11
/product="LacZ"
/protein_id="AGT62527.1"
/db_xref="GI:531997063"
/translation="MTMITPSAQLTLTKGNKSWVPGPPSRSTVSISLISNSCSPGDPL
VLERPPPRWSSNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
ARTDRPSQQLRSLNGEWKL"
gene 3682..4485
/gene="strA"
CDS 3682..4485
/gene="strA"
/codon_start=1
/transl_table=11
/product="Sm resistance protein A"
/protein_id="AGT62528.1"
/db_xref="GI:531997064"
/translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLL
ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
gene 4485..5321
/gene="strB"
CDS 4485..5321
/gene="strB"
/codon_start=1
/transl_table=11
/product="Sm resistance protein B"
/protein_id="AGT62529.1"
/db_xref="GI:531997065"
/translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
SY"
ORIGIN
1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
3301 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
3361 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
3421 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
3481 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
3541 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcatt
3601 catgtttgac agcttatcat ccataaggtt taatgcggta gtttatcaca gttaaattgc
3661 taacgcagtc aggcaccgtg tatgaatcga actaatattt tttttggtga atcgcattct
3721 gactggttgc ctgtcagagg cggagaatct ggtgattttg tttttcgacg tggtgacggg
3781 catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg agctcgctgg agagcgtgac
3841 cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg aggtcatcaa ctggcaggag
3901 gaacaggagg gtgcatgctt ggtgataacg gcaattccgg gagtaccggc ggctgatctg
3961 tctggagcgg atttgctcaa agcgtggccg tcaatggggc agcaacttgg cgctgttcac
4021 agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt cgcgaatgtt cggacgcgcc
4081 gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct taccggacga ggacaagagt
4141 acgccgctgc acgatctttt ggctcgtgtc gaacgagagc taccggtgcg gctcgaccaa
4201 gagcgcaccg atatggttgt ttgccatggt gatccctgca tgccgaactt catggtggac
4261 cctaaaactc ttcaatgcac gggtctgatc gaccttgggc ggctcggaac agcagatcgc
4321 tatgccgatt tggcactcat gattgctaac gccgaagaga actgggcagc gccagatgaa
4381 gcagagcgcg ccttcgctgt cctattcaat gtattgggga tcgaagcccc cgaccgcgaa
4441 cgccttgcct tctatctgcg attggaccct ctgacttggg gttgatgttc atgccgcctg
4501 tttttcctgc tcattggcac gtttcgcaac ctgttctcat tgcggacacc ttttccagcc
4561 tcgtttggaa agtttcattg ccagacggga ctcctgcaat cgtcaaggga ttgaaaccta
4621 tagaagacat tgctgatgaa ctgcgcgggg ccgactatct ggtatggcgc aatgggaggg
4681 gagcagtccg gttgctcggt cgtgagaaca atctgatgtt gctcgaatat gccggggagc
4741 gaatgctctc tcacatcgtt gccgagcacg gcgactacca ggcgaccgaa attgcagcgg
4801 aactaatggc gaagctgtat gccgcatctg aggaacccct gccttctgcc cttctcccga
4861 tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga tgatcaaaac gcaggttgtc
4921 aaactgacta cgtccacgcg gcgattatag ccgatcaaat gatgagcaat gcctcggaac
4981 tgcgtgggct acatggcgat ctgcatcatg aaaacatcat gttctccagt cgcggctggc
5041 tggtgataga tcccgtcggt ctggtcggtg aagtgggctt tggcgccgcc aatatgttct
5101 acgatccggc tgacagagac gacctttgtc tcgatcctag acgcattgca cagatggcgg
5161 acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct gctcgaccag gcgtacgctt
5221 atgggtgcct ttccgcagct tggaacgcgg atggagaaga ggagcaacgc gatctagcta
5281 tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc gacttctcct
5341 atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc ttagcgtagg
5401 attccgcccc ttcccgcaaa cgaccccaaa caagatcttg atcccctgcg ccatcagatc
5461 cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc
5521 gccccagctg gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc
5581 catgtaagcc cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag
5641 atagcccagt agctgacatt catcccaggt ggcacttttc ggggaaatgt gcgcgcccgc
5701 gttcctgctg gcgctgggcc tgtttctggc gctggacttc ccgctgttcc gtcagcagct
5761 tttcgcccac ggccttgatg atcgcggcgg ccttggcctg catatcccga ttcaacggcc
5821 ccagggcgtc cagaacgggc ttcaggcgct cccgaaggt
//
