| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBTB-5 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 3161 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 甲氧氨苄嘧啶 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBTB-5载体质粒基本信息
pBTB-5质粒图谱载体图谱和pBTB-5载体序列质粒序列多克隆位点信息
pBTB-5质粒载体简介
pBTB-5质粒序列载体序列
LOCUS DQ058733 3161 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBTB-5, complete sequence.
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..3161
/organism="Broad host range vector pBTB-5"
/mol_type="other DNA"
/db_xref="taxon:334965"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene 872..1534
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 872..1534
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99729.1"
/db_xref="GI:69048360"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 1631..1867
/gene="dhfrIIb"
/note="dhfrIIb gene from R388"
CDS 1631..1867
/gene="dhfrIIb"
/codon_start=1
/transl_table=11
/product="dihydrofolate reductase"
/protein_id="AAY99730.1"
/db_xref="GI:69048361"
/translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG
WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
regulatory 1884..1915
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(1943..2872)
/gene="araC"
CDS complement(1943..2872)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99731.1"
/db_xref="GI:69048362"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 2880..3147
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1621 ggaagctaaa atgggtcaaa gtagcgatga agccaacgct cccgttgcag ggcagtttgc
1681 gcttcccctg agtgccacct ttggcttagg ggatcgcgta cgcaagaaat ctggtgccgc
1741 ttggcagggt caagtcgtcg gttggtattg cacaaaactc actcctgaag gctatgcggt
1801 cgagtccgaa tcccacccag gctcagtgca aatttatcct gtggctgcac ttgaacgtgt
1861 ggcctaagaa tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg
1921 ctatggaggt caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt
1981 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
2041 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
2101 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
2161 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
2221 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
2281 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
2341 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
2401 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
2461 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
2521 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
2581 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
2641 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
2701 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
2761 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
2821 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
2881 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
2941 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
3001 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
3061 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
3121 ctgtttctcc atacccgttt ttttgggcta gagaattcgt c
//
