| 出品人: | Fodor,B.D.(2004) |
|---|---|
| 载体名称: | pMHE3 |
| 质粒类型: | 广宿主载体；细菌表达载体;体外表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7/lac |
| 载体大小: | 6290 bp |
| 5' 测序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | T7 tag (N ter)； 6XHis tag (C ter) |
| 载体抗性: | 链霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pMHE3载体质粒基本信息
pMHE3质粒图谱载体图谱和pMHE3载体序列质粒序列多克隆位点信息
pMHE3质粒载体简介
pMHE3质粒序列载体序列
LOCUS AY299694 6290 bp DNA circular SYN 10-FEB-2004
DEFINITION Broad host range expression vector pMHE3, complete sequence.
ACCESSION AY299694
VERSION AY299694.1 GI:34334142
TITLE Modular broad-host-range expression vectors for single-protein and
protein complex purification
JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
PUBMED 14766546
AUTHORS Fodor,B.D.
JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of
Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
FEATURES Location/Qualifiers
source 1..6290
/organism="Broad host range expression vector pMHE3"
/mol_type="genomic DNA"
/db_xref="taxon:244052"
primer_bind 111..129
/note="T7 promoter primer"
regulatory 111..127
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 128..153
/note="lac operator"
regulatory 184..190
/regulatory_class="ribosome_binding_site"
misc_feature 199..231
/note="T7-tag"
misc_feature 280..297
/note="His-tag"
primer_bind complement(350..367)
/note="T7 terminator primer"
regulatory 363..410
/regulatory_class="terminator"
/note="T7 terminator"
rep_origin 1436..2098
/note="pBBR1 oriV"
misc_feature 3092..4085
/note="RP4 mob; oriT"
regulatory complement(4175..4290)
/regulatory_class="terminator"
/note="T4 terminator"
gene complement(4550..5341)
/gene="aadA"
CDS complement(4550..5341)
/gene="aadA"
/note="streptomycin resistance"
/codon_start=1
/transl_table=11
/product="aminoglycoside-3:adenyltransferase fusion"
/protein_id="AAQ64665.1"
/db_xref="GI:34334143"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
regulatory 6077..6192
/regulatory_class="terminator"
/note="T4 terminator"
ORIGIN
1 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
61 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
121 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
181 taagaaggag atatacatat ggctagcatg actggtggac agcaaatggg tcgggatccg
241 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga
301 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
421 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
841 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatattt tgttaaaatt
901 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgact gcgatgagtg
961 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac
1021 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg
1081 tcgtcggttc agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt
1141 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc
1201 aggctctccc cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagagcc
1261 tgataaaaac ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
1321 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgaggcg
1381 gctacagccg atagtctgga acagcgcact tacgggttgc tgcgcaaccc aagtgctacc
1441 ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc gccatcgtcc agaaaacacg
1501 gctcatcggg catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca
1561 aggctggcag gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg
1621 ggccggtcgg tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat
1681 gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg
1741 acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg
1801 acacggtgcc ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct
1861 tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc
1921 tgaccaccac ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg
1981 ccgtgggttt cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca
2041 cccagtgacc gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc
2101 ttatctccat gcggtagggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt
2161 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct
2221 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt
2281 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc
2341 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa
2401 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg
2461 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg
2521 cttcgccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca
2581 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca
2641 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat
2701 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag
2761 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc
2821 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg
2881 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag
2941 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag
3001 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga
3061 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa
3121 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga
3181 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac
3241 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt
3301 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga
3361 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga
3421 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt
3481 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca
3541 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg
3601 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg
3661 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc
3721 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc
3781 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg
3841 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga
3901 gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc aggccaaggc
3961 cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg
4021 ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg aaaagtgcca
4081 cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg atctcggctt
4141 gaacgaattg ttaggtggcg gtacttgggt cgatagcttt atgcttgtaa accgttttgt
4201 gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt agtaatataa
4261 tctattaaag gtcattcaaa aggtcatcca ccggatcagc ttagtaaagc cctcgctaga
4321 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
4381 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
4441 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg
4501 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga
4561 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
4621 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
4681 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
4741 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
4801 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
4861 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
4921 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
4981 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
5041 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
5101 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
5161 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
5221 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
5281 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
5341 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
5401 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
5461 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
5521 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct
5581 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
5641 gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg
5701 agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg
5761 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc
5821 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag
5881 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt
5941 tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca
6001 agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc
6061 aggggaattg atccggtgga tgaccttttg aatgaccttt aatagattat attactaatt
6121 aattggggac cctagaggtc ccctttttta ttttaaaaat tttttcacaa aacggtttac
6181 aagcataaag ctcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
6241 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
//
