出品公司: | Addgene |
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载体名称: | PX393 或 PX393 Gluconacetobacter diazotrophicus Cas9 |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 10346 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3') |
载体标签: | NLS (C ter);HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞(系): | 昆虫细胞 |
备注: | -- |
产品目录号: | #68330 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
买家导航
PX393载体质粒基本信息
PX393质粒图谱载体图谱和PX393载体序列质粒序列多克隆位点信息
PX393质粒载体简介
PX393质粒序列载体序列
LOCUS PX393 Gluconacetobacter diazotrophicus Cas9 10346 bp DNA SYN DEFINITION PX393 Gluconacetobacter diazotrophicus Cas9 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10346 /organism="PX393 Gluconacetobacter diazotrophicus Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5037 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMGENMIDESLTFGIDLGIGSCGW AVLRRPSAFGRKGVIEGMGSWCFDVPETSKERTPTNQIRRSNRLLRRVIRRRRNRMAA IRRLLHAAGLLPSTDSDALKRPGHDPWELRARGLDKPLKPVEFAVVLGHIAKRRGFKS AAKRKATNISSDDKKMLTALEATRERLGRYRTVGEMFARDPDFASRRRNREGKYDRTT ARDDLEHEVHALFAAQRRLGQGFASPELEEAFTASAFHQRPMQDSERLVGFCPFERTE KRAAKLTPSFERFRLLARLLNLRITTPDGERPLTVDEIALVTRDLGKTAKLSIKRVRT LIGLEDNQRFTTIRPEDEDRDIVARTGGAMTGTATLRKALGEALWTDMQERPEQLDAI VQVLSFFEANETITEKLREIGLTLAVLDVLLTALDAGVFAKFKGAAHISTKAARNLLP HLEQGRRYDEACTMAGYDHAASRLSHHGQIVAKTQFNALVTEIGESIANPIARKALIE GLKQIWAMRNHWGLPGSIHVELARDVGNSIEKRREIEKHIEKNTALRARERREVHDLL DLEDVNGDTLLRYRLWKEQGGKCLYTGKAIHIRQIAATDNSVQVDHILPWSRFGDDSF NNKTLCLASANQQKKRSTPYEWLSGQTGDAWNAFVQRIETNKELRGFKKRNYLLKNAK EAEEKFRSRNLNDTRYAARLFAEAVKLLYAFGERQEKGGNRRVFTRPGALTAALRQAW GVESLKKQDGKRINDDRHHALDALTVAAVDEAEIQRLTKSFHEWEQQGLGRPLRRVEP PWESFRADVEATYPEVFVARPERRRARGEGHAATIRQVKERECTPIVFERKAVSSLKE ADLERIKDGERNEAIVEAIRSWIATGRPADAPPRSPRGDIITKIRLATTIKAAVPVRG GTAGRGEMVRADVFSKPNRRGKDEWYLVPVYPHQIMNRKAWPKPPMRSIVANKDEDEW TEVGPEHQFRFSLYPRSNIEIIRPSGEVIEGYFVGLHRNTGALTISAHNDPKSIHSGI GTKTLLAISKYQVDRFGRKSPVRKEVRTWHGEACISPTPPG*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMGENMIDESLTFGIDLGIGSCGW AVLRRPSAFGRKGVIEGMGSWCFDVPETSKERTPTNQIRRSNRLLRRVIRRRRNRMAA IRRLLHAAGLLPSTDSDALKRPGHDPWELRARGLDKPLKPVEFAVVLGHIAKRRGFKS AAKRKATNISSDDKKMLTALEATRERLGRYRTVGEMFARDPDFASRRRNREGKYDRTT ARDDLEHEVHALFAAQRRLGQGFASPELEEAFTASAFHQRPMQDSERLVGFCPFERTE KRAAKLTPSFERFRLLARLLNLRITTPDGERPLTVDEIALVTRDLGKTAKLSIKRVRT LIGLEDNQRFTTIRPEDEDRDIVARTGGAMTGTATLRKALGEALWTDMQERPEQLDAI VQVLSFFEANETITEKLREIGLTLAVLDVLLTALDAGVFAKFKGAAHISTKAARNLLP HLEQGRRYDEACTMAGYDHAASRLSHHGQIVAKTQFNALVTEIGESIANPIARKALIE GLKQIWAMRNHWGLPGSIHVELARDVGNSIEKRREIEKHIEKNTALRARERREVHDLL DLEDVNGDTLLRYRLWKEQGGKCLYTGKAIHIRQIAATDNSVQVDHILPWSRFGDDSF NNKTLCLASANQQKKRSTPYEWLSGQTGDAWNAFVQRIETNKELRGFKKRNYLLKNAK EAEEKFRSRNLNDTRYAARLFAEAVKLLYAFGERQEKGGNRRVFTRPGALTAALRQAW GVESLKKQDGKRINDDRHHALDALTVAAVDEAEIQRLTKSFHEWEQQGLGRPLRRVEP PWESFRADVEATYPEVFVARPERRRARGEGHAATIRQVKERECTPIVFERKAVSSLKE ADLERIKDGERNEAIVEAIRSWIATGRPADAPPRSPRGDIITKIRLATTIKAAVPVRG GTAGRGEMVRADVFSKPNRRGKDEWYLVPVYPHQIMNRKAWPKPPMRSIVANKDEDEW TEVGPEHQFRFSLYPRSNIEIIRPSGEVIEGYFVGLHRNTGALTISAHNDPKSIHSGI GTKTLLAISKYQVDRFGRKSPVRKEVRTWHGEACISPTPPG*" CDS complement(1845..2327) /label="ORF frame 1" /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA *" misc_feature 1855..1875 /label="SV40 NLS" /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA *" misc_feature 1861..1875 /label="NLS" /translation="MFVAFLFAADLNPLRFAMWPRTTANSTGFSGLSRPRALSSQGSC PGRFRASLSVDGSRPAACSSRRIAAIRFLLRRITRLSSLLDLRIWLVGVRSLDVSGTS KHQEPIPSITPFRPKALGRLSTAHPQLPIPRSMPKVRLSSIIFSPMDASTLRFFFGEA *" CDS complement(2578..3402) /label="ORF frame 3" /translation="MDTSGQPPVVSHGPNLFQPLDQCLPSDWIGNGFADLSDQGVELC LCHDLAMVRQAGGCVVIPCHCAGLIVAPALLQMWQQIPSGFGADVCSAFKLGEHSGIQ CGQQDVQHGQSQANFPQLLSDCFVGLKEAQDLHDSVQLFRALLHISPQGLSQCLPQGG SPCHCPACPSHNVSIFVLGADSCEPLIVLQSNQSSHPLDTQFCGLAQVPGDQSNFIHC QGPLSVWGSDSQVQQPGQQSKALERGCQFGCPLFGSLKRAESHQALAVLHGPLMKC*" CDS complement(4251..4934) /label="ORF frame 1" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" misc_feature complement(5070..5087) /label="Sp6_primer" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" misc_feature 5092..5116 /label="attB2" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" terminator 5128..5594 /label="hGH_PA_terminator" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" terminator 5600..5635 /label="T7_terminator" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" rep_origin 5752..6058 /label="f1_origin" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" misc_feature complement(6172..6192) /label="pBABE_3_primer" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" misc_feature complement(6178..6393) /label="SV40_enhancer" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" promoter 6190..6458 /label="SV40_promoter" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" rep_origin 6357..6434 /label="SV40_origin" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" misc_feature 6419..6438 /label="SV40pro_F_primer" /translation="MASSVLVPMPEWILLGSLCALMVRAPVLRCRPTKYPSITSPDGL MISILDLGYRLNRNWCSGPTSVHSSSSLLATIERIGGLGQAFLFMIWCGYTGTRYHSS FPLRFGLLNTSARTISPLPAVPPRTGTAALMVVASLILVMMSPRGERGGASAGRPVAI QLLMASTIASLRSPSLIRSRSASFRLETAFLSNTIGVHSLSFTCRMVAAWPSPLARRL SGRATNTSG*" CDS 6573..7367 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6576..7364 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(6882..7418) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7544..7663 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7632..7651 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7854..7889 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(7912..7929) /label="Baculovirs_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7919..7996 /label="3_AcPH" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" CDS complement(8026..8643) /label="ORF frame 3" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 8623..8656 /label="loxP" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" gene complement(9424..10284) /label="Ampicillin" /gene="Ampicillin" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" CDS complement(9424..10284) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag 1861 aaaaagcgca aggtcgaagc gtccatggga gagaatatga ttgacgagag tctgaccttc 1921 ggcattgacc tggggattgg gagttgtggg tgggcagtgc tgaggcggcc aagcgccttc 1981 ggacggaagg gcgtgatcga gggaatgggc tcctggtgct ttgacgtccc cgagacatct 2041 aaagaacgga ctcctaccaa ccagatccgg agatccaata gactgctgag gcgcgtgatc 2101 cgacggagaa ggaaccggat ggccgctatt cgccgactgc tgcacgcagc aggcctgctg 2161 ccatcaaccg acagcgatgc cctgaagcgg cccggacatg atccttggga actgagggca 2221 cgaggcctgg acaagccact gaaacccgtg gagttcgctg tggtcctggg ccacatcgca 2281 aagcggagag ggtttaaatc cgctgcaaag agaaaagcca caaacattag ctccgacgat 2341 aagaaaatgc tgacagccct ggaggctact cgagaacggc tggggagata caggaccgtg 2401 ggagaaatgt tcgccaggga ccctgatttt gcttctaggc gccgaaatcg cgagggcaag 2461 tatgatagga ccacagctcg cgacgatctg gagcacgaag tgcacgccct gttcgcagct 2521 cagcggagac tgggacaggg atttgccagt ccagaactgg aggaggcctt caccgcttca 2581 gcatttcatc agaggcccat gcaggacagc gagcgcctgg tgggattctg cccttttgag 2641 cgaaccgaaa agcgggcagc caaactgaca ccctctttcg agcgctttcg actgctggcc 2701 cggctgctga acctgagaat cactacccca gacggagagc ggcccctgac agtggatgaa 2761 attgctctgg tcacccggga cctgggcaag accgcaaaac tgagtatcaa gcgggtgaga 2821 actctgattg gactggagga caatcagagg ttcacaacta tccgccccga ggacgaagat 2881 cgagacattg tggctcggac aggcggggca atgacaggga ctgccaccct gaggaaggca 2941 ctgggagagg ccctgtggac tgatatgcag gagcgccctg aacagctgga cgctatcgtg 3001 caggtcctga gcttctttga ggccaacgaa acaatcactg agaagctgag ggaaattggc 3061 ctgactctgg ccgtgctgga cgtcctgctg accgcactgg atgccggagt gttcgccaag 3121 tttaaaggcg ctgcacacat cagcaccaaa gccgctagga atctgctgcc acatctggag 3181 cagggcaggc gctacgatga ggcctgcaca atggcagggt atgaccacgc agcctcccgc 3241 ctgtctcacc atggccagat cgtggcaaag acacagttca acgccctggt cactgagatc 3301 ggcgaatcca ttgccaatcc aatcgctcgg aaggcactga tcgaggggct gaaacagatt 3361 tgggccatga gaaaccactg ggggctgccc ggaagtatcc atgtggagct ggcccgggat 3421 gtcggcaact caattgaaaa gcgacgggag attgaaaagc acatcgagaa aaatactgcc 3481 ctgagggctc gcgagagaag ggaggtgcat gatctgctgg acctggaaga tgtcaatggc 3541 gacaccctgc tgcgataccg gctgtggaag gagcagggag gcaaatgcct gtatacaggg 3601 aaggccatcc acattcggca gatcgctgca actgacaact ccgtgcaggt cgatcatatt 3661 ctgccttgga gccggttcgg cgacgatagt tttaacaaca agaccctgtg tctggcctct 3721 gctaatcagc agaagaaaag gtcaacacca tacgagtggc tgagcggcca gactggggat 3781 gcatggaacg ccttcgtgca gcgcatcgag acaaataagg aactgagagg gtttaagaaa 3841 aggaactatc tgctgaagaa tgctaaagag gcagaggaaa aattcagaag caggaacctg 3901 aatgacacca gatacgccgc taggctgttc gcagaggccg tgaagctgct gtatgccttt 3961 ggggagagac aggaaaaagg gggaaaccgc cgagtgttta ctcggcctgg agcactgacc 4021 gcagcactga gacaggcttg gggagtggag agcctgaaga aacaggatgg gaagcgcatc 4081 aatgacgatc gacaccatgc cctggatgct ctgaccgtgg ctgcagtcga cgaggccgaa 4141 attcagaggc tgacaaaatc attccacgag tgggaacagc agggcctggg gcggcctctg 4201 cggagagtgg agccaccttg ggagagcttc cgggcagacg tcgaggctac ctaccctgaa 4261 gtgtttgtcg cacggccaga gaggcgccga gcaagaggag aaggccatgc cgctaccatc 4321 cggcaggtga aggagagaga atgcacacca attgtgtttg agagaaaggc tgtctctagt 4381 ctgaaagagg cagacctgga acgaatcaaa gatggcgagc gcaacgaagc aattgtggag 4441 gccatcagga gctggattgc cactggacgc ccagctgatg caccaccacg ctccccccga 4501 ggcgacatca ttaccaagat caggctggcc accaccatca aggcagccgt gcctgtccgc 4561 ggagggaccg caggaagggg agaaatggtg cgcgcagatg tgttcagcaa gccaaaccgg 4621 agagggaaag acgagtggta tctggtgccc gtgtatccac accagatcat gaacaggaag 4681 gcttggccca aacctccaat gcgctcaatt gtggccaata aggatgagga cgaatggacc 4741 gaagtgggac ctgaacacca gttccggttt agcctgtacc ctagatccaa tatcgagatc 4801 attaggccat ctggagaagt gatcgaagga tatttcgtcg gcctgcatcg caacactggc 4861 gctctgacca tcagtgcaca caatgatccc aagagtatcc attcaggcat tgggaccaag 4921 acactgctgg ccatttccaa ataccaggtg gacagattcg gcagaaagtc tccagtgcgc 4981 aaagaggtcc gaacttggca cggggaagcc tgtatctctc ccaccccccc tggataagaa 5041 ttcggtaccg agctcaagct tgcggccgcc tatagtgtca cctaaatcgt aacccagctt 5101 tcttgtacaa agtggtcggg tggcatccct gtgacccctc cccagtgcct ctcctggccc 5161 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt 5221 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg 5281 ggcaagttgg gatgacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg 5341 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc 5401 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt 5461 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc 5521 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc 5581 ccttccctgt ccttctagca taaccccttg gggcctctaa acgggtcttg aggggttttt 5641 tgtctagaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 5701 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 6181 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 6241 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 6301 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 6361 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 6421 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 6481 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 6541 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 6601 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 6661 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 6721 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 6781 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 6841 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 6901 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 6961 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 7021 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 7081 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 7141 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 7201 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 7261 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 7321 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 7381 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 7441 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 7501 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 7561 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7621 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gttcatgaag 7681 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 7741 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat 7801 cagaatgagt atttggttta gagtttggca acatatgccc atatgtaact agcataaccc 7861 cttggggcct ctaaacgggt cttgaggggt tttttgctga aagcatgcgg aggaaattct 7921 ccttgaagtt tccctggtgt tcaaagtaaa ggagtttgca ccagacgcac ctctgttcac 7981 tggtccggcg tattaaaaca cgatacattg ttattagtac atttattaag cgctagattc 8041 tgtgcgttgt tgatttacag acaattgttg tacgtatttt aataattcat taaatttata 8101 atctttaggg tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa 8161 tttaaatatt aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg 8221 ttgtttttcc gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact 8281 tgccaaatct tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa 8341 taaaggttcg acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt 8401 agtgtacaat tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg 8461 cgtgttagct ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc 8521 ttccgaagac gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc 8581 cgcgatcaaa tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata 8641 cattatacga agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt 8701 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8761 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8821 gtttttccat aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 8881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 8941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 9001 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 9061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 9121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 9181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 9241 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 9301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 9361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 9421 ttgttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 9961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 10201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 10261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 10321 gggttattgt ctcatgtccg cgcgtt //