出品公司: | Addgene |
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载体名称: | PX398 或 PX398 Nitratifractor salsuginis str DSM 16511 Cas9 |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 10592 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | NLS (C ter);HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞(系): | 昆虫细胞 |
备注: | -- |
产品目录号: | #68334 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
买家导航
PX398载体质粒基本信息
PX398质粒图谱载体图谱和PX398载体序列质粒序列多克隆位点信息
PX398质粒载体简介
PX398质粒序列载体序列
LOCUS PX398 Nitratifractor salsuginis str DSM 16511 Cas9 10592 bp DNA SYN DEFINITION PX398 Nitratifractor salsuginis str DSM 16511 Cas9 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10592 /organism="PX398 Nitratifractor salsuginis str DSM 16511 Cas9" /mol_type="other DNA" misc_feature 47..1001 /label="Ac_5_flank" CDS complement(343..948) /label="ORF frame 3" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1023..1587 /label="CMV_immearly_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1090..1377 /label="CAG_enhancer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1544..1564 /label="CMV_fwd_primer" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" promoter 1615..1633 /label="T7_promoter" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1633..1656 /label="lacO" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" misc_feature 1772..1796 /label="attB1" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*" CDS 1822..5283 /label="ORF frame 1" /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR VEDDPHH*" misc_feature 1825..1851 /label="HA" /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR VEDDPHH*" misc_feature 1855..1875 /label="SV40 NLS" /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR VEDDPHH*" misc_feature 1861..1875 /label="NLS" /translation="MYPYDVPDYASPKKKRKVEASMKKILGVDLGITSFGYAILQETG KDLYRCLDNSVVMRNNPYDEKSGESSQSIRSTQKSMRRLIEKRKKRIRCVAQTMERYG ILDYSETMKINDPKNNPIKNRWQLRAVDAWKRPLSPQELFAIFAHMAKHRGYKSIATE DLIYELELELGLNDPEKESEKKADERRQVYNALRHLEELRKKYGGETIAQTIHRAVEA GDLRSYRNHDDYEKMIRREDIEEEIEKVLLRQAELGALGLPEEQVSELIDELKACITD QEMPTIDESLFGKCTFYKDELAAPAYSYLYDLYRLYKKLADLNIDGYEVTQEDREKVI EWVEKKIAQGKNLKKITHKDLRKILGLAPEQKIFGVEDERIVKGKKEPRTFVPFFFLA DIAKFKELFASIQKHPDALQIFRELAEILQRSKTPQEALDRLRALMAGKGIDTDDREL LELFKNKRSGTRELSHRYILEALPLFLEGYDEKEVQRILGFDDREDYSRYPKSLRHLH LREGNLFEKEENPINNHAVKSLASWALGLIADLSWRYGPFDEIILETTRDALPEKIRK EIDKAMREREKALDKIIGKYKKEFPSIDKRLARKIQLWERQKGLDLYSGKVINLSQLL DGSADIEHIVPQSLGGLSTDYNTIVTLKSVNAAKGNRLPGDWLAGNPDYRERIGMLSE KGLIDWKKRKNLLAQSLDEIYTENTHSKGIRATSYLEALVAQVLKRYYPFPDPELRKN GIGVRMIPGKVTSKTRSLLGIKSKSRETNFHHAEDALILSTLTRGWQNRLHRMLRDNY GKSEAELKELWKKYMPHIEGLTLADYIDEAFRRFMSKGEESLFYRDMFDTIRSISYWV DKKPLSASSHKETVYSSRHEVPTLRKNILEAFDSLNVIKDRHKLTTEEFMKRYDKEIR QKLWLHRIGNTNDESYRAVEERATQIAQILTRYQLMDAQNDKEIDEKFQQALKELITS PIEVTGKLLRKMRFVYDKLNAMQIDRGLVETDKNMLGIHISKGPNEKLIFRRMDVNNA HELQKERSGILCYLNEMLFIFNKKGLIHYGCLRSYLEKGQGSKYIALFNPRFPANPKA QPSKFTSDSKIKQVGIGSATGIIKAHLDLDGHVRSYEVFGTLPEGSIEWFKEESGYGR VEDDPHH*" CDS complement(1981..2502) /label="ORF frame 3" /translation="MVSIRSQVASFDCPVNCLGDCLPAVFLAQFLQVPQSIIHLAPLI GFLLAFLLWVVQPQFQLQFVDQILCGDTFIASMLCHVSEDGKQLLRRQGPLPCVHSSQ LPPILDGIVLWVVDFHSLRVIQNAISFHCLGHASNPFLPFLDQSPHGLLGTADRLGAL SALFIVGIVPHDH*" CDS complement(4435..5280) /label="ORF frame 3" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" misc_feature complement(5316..5333) /label="Sp6_primer" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" misc_feature 5338..5362 /label="attB2" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" terminator 5374..5840 /label="hGH_PA_terminator" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" terminator 5846..5881 /label="T7_terminator" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" rep_origin 5998..6304 /label="f1_origin" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" misc_feature complement(6418..6438) /label="pBABE_3_primer" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" misc_feature complement(6424..6639) /label="SV40_enhancer" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" promoter 6436..6704 /label="SV40_promoter" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" rep_origin 6603..6680 /label="SV40_origin" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" misc_feature 6665..6684 /label="SV40pro_F_primer" /translation="MVRIVLHAPVATLFLEPLDRPFGQGAKDLIAPHMSIQVQVGLND PCCRSNPHLFDLGVAGEFGRLCLRISGEAWVKQGDVFGALSLLQVAPQTSIVNQPFLI EDKQHLVQIAQNATSLLLQFMSIVDIHSAKDQFFIGPLRNVDAQHVFVCLHQSPIDLH GIQLVVDESHLPQQFPSHFNWGCDQFLQCLLKFLVDFLIVLSVHQLVSCQNLGDLGCS LFHGPIAFIIGVANSMQPQLLPDLLVVSFHELFGSQLVPVLDHIQGIKSLQDVFSQGW DLMPA*" CDS 6819..7613 /label="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 6822..7610 /label="NeoR/KanR" /gene="NeoR/KanR" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" CDS complement(7128..7664) /label="ORF frame 1" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 7790..7909 /label="SV40_PA_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 7878..7897 /label="EBV_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" terminator 8100..8135 /label="T7_terminator" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature complement(8158..8175) /label="Baculovirs_rev_primer" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" misc_feature 8165..8242 /label="3_AcPH" /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ PPHCISHDGYFLGRSKVR*" CDS complement(8272..8889) /label="ORF frame 3" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" misc_feature 8869..8902 /label="loxP" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" gene complement(9670..10530) /label="Ampicillin" /gene="Ampicillin" /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*" CDS complement(9670..10530) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag 1861 aaaaagcgca aggtcgaagc gtccatgaaa aagattctgg gggtggacct gggcatcaca 1921 tcattcggct acgctattct gcaggagact ggaaaggacc tgtatcggtg cctggacaac 1981 tcagtggtca tgcggaacaa tccctacgat gaaaagagcg gagagagctc ccagtctatc 2041 cgcagtaccc agaagtccat gcggagactg atcgagaaac ggaagaaacg gattagatgc 2101 gtggcccaga caatggaaag atatggcatt ctggattact ctgagactat gaaaatcaac 2161 gacccaaaga acaatcccat caagaatagg tggcagctgc gagctgtgga cgcatggaag 2221 cggcccctgt ctcctcagga gctgtttgcc atcttcgctc acatggcaaa gcatagaggc 2281 tataaaagta tcgccacaga ggatctgatc tacgaactgg agctggaact ggggctgaac 2341 gacccagaga aggaaagcga gaagaaagcc gatgagaggc gccaggtgta taatgctctg 2401 aggcacctgg aggaactgcg caagaaatac ggcggggaga caatcgccca gacaattcac 2461 agggcagtcg aagctggcga cctgcgatct tatcgaaacc atgacgatta cgaaaagatg 2521 atccgacggg aggatatcga ggaagagatt gaaaaagtgc tgctgcgaca ggcagagctg 2581 ggagctctgg gcctgcccga agagcaggtg agcgaactga tcgacgagct gaaggcctgc 2641 attactgatc aggaaatgcc aaccatcgac gagagcctgt ttggcaagtg taccttctat 2701 aaagacgagc tggccgctcc cgcctactcc tatctgtacg atctgtatag gctgtacaag 2761 aaactggctg acctgaacat cgatggctac gaggtgacac aggaagatcg cgagaaagtg 2821 atcgaatggg tcgagaagaa aattgctcag gggaaaaatc tgaagaaaat cactcacaag 2881 gacctgagaa aaattctggg actggcacct gagcagaaga tctttggcgt ggaagatgag 2941 agaattgtca aagggaagaa agaacctagg actttcgtgc cattcttttt cctggcagac 3001 atcgccaagt ttaaagagct gttcgcctca attcagaagc atcccgatgc tctgcagatc 3061 tttagggaac tggcagagat tctgcagcgc agcaagaccc ctcaggaggc cctggacagg 3121 ctgcgcgctc tgatggcagg gaaaggaatc gatactgacg atcgggaact gctggagctg 3181 ttcaagaaca aacgatcagg cacccgggag ctgagccaca gatatatcct ggaagctctg 3241 ccactgtttc tggagggcta cgacgaaaag gaggtgcaga ggattctggg gttcgacgat 3301 agagaggatt atagcagata ccccaagtca ctgcgccacc tgcatctgcg agaagggaac 3361 ctgtttgaga aggaagagaa tccaatcaac aatcatgcag tgaaatcact ggccagctgg 3421 gctctgggac tgattgccga tctgagctgg cgctacggcc cattcgacga aatcatcctg 3481 gagacaacac gagatgccct gcccgaaaag atccggaaag agattgacaa ggctatgcga 3541 gaacgggaga aggcactgga taagatcatc gggaagtaca agaaggagtt ccctagtatc 3601 gacaagcggc tggcaagaaa aattcagctg tgggaaaggc agaagggact ggatctgtac 3661 tcaggcaaag tgatcaatct gtcccagctg ctggacggat ctgctgatat cgagcacatt 3721 gtcccacaga gcctgggagg cctgtccacc gactataaca ccatcgtgac actgaagtcc 3781 gtcaacgcag ccaaagggaa tcggctgccc ggagattggc tggccggcaa tcctgactac 3841 agagaaagga tcgggatgct gagtgagaag ggactgattg actggaagaa aagaaaaaac 3901 ctgctggcac agtcactgga tgaaatctat actgagaata cccacagtaa gggcattagg 3961 gccacatcat acctggaggc actggtggcc caggtcctga agcgctacta tccctttcct 4021 gacccagaac tgagaaaaaa cggcatcggg gtgaggatga ttcccggaaa agtcacatcc 4081 aagactcgct ctctgctggg catcaagtcc aaatctcgag aaactaattt ccaccatgct 4141 gaggacgcac tgattctgtc cacactgact agggggtggc agaacaggct gcaccggatg 4201 ctgcgagata attacggaaa gtctgaagcc gagctgaaag aactgtggaa gaaatatatg 4261 cctcatatcg agggactgac cctggccgac tacattgatg aggccttccg gaggttcatg 4321 tctaagggcg aagagagtct gttttatcgc gacatgttcg atacaatccg aagtatttca 4381 tactgggtgg acaagaaacc tctgagcgcc tctagtcaca aggaaactgt gtattcaagc 4441 cggcatgagg tcccaaccct gagaaaaaac atcctggagg cttttgattc cctgaatgtg 4501 atcaaggacc ggcacaagct gactaccgaa gagttcatga aacgatacga caaggagatc 4561 cggcagaagc tgtggctgca tagaattggc aacaccaatg atgaaagcta tcgggccgtg 4621 gaagagagag caacccagat cgcccagatt ctgacaagat accagctgat ggacgctcag 4681 aacgataagg aaatcgacga gaaatttcag caggcactga aggaactgat cacatcccca 4741 attgaagtga ctgggaaact gctgcggaag atgagattcg tctacgacaa gctgaatgcc 4801 atgcagatcg atcggggact ggtggagaca gacaaaaaca tgctgggcat ccacatttct 4861 aaggggccca atgaaaaact gatctttcgc cgaatggatg tcaacaatgc tcatgaactg 4921 cagaaggaga gaagtggcat tctgtgctat ctgaacgaga tgctgtttat cttcaataag 4981 aaagggctga ttcactatgg atgtctgagg agctacctgg agaagggaca gggctccaaa 5041 tacatcgccc tgtttaaccc acgcttcccc gctaatccta aggcacagcc ttccaaattc 5101 accagcgact ccaagatcaa acaagtgggg attggatctg caacagggat cattaaggcc 5161 cacctggacc tggatggaca tgtgaggagc tatgaggtct ttggcaccct gcccgaaggg 5221 tctatcgagt ggttcaagga agagagtggc tacgggcgcg tggaggacga tcctcaccat 5281 taagaattcg gtaccgagct caagcttgcg gccgcctata gtgtcaccta aatcgtaacc 5341 cagctttctt gtacaaagtg gtcgggtggc atccctgtga cccctcccca gtgcctctcc 5401 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca 5461 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga 5521 gcaaggggca agttgggatg acaacctgta gggcctgcgg ggtctattgg gaaccaagct 5581 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc 5641 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat 5701 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct 5761 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca 5821 ctgctccctt ccctgtcctt ctagcataac cccttggggc ctctaaacgg gtcttgaggg 5881 gttttttgtc tagaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 5941 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 6001 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 6061 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 6121 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 6181 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 6241 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 6301 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 6361 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 6421 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 6481 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 6541 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 6601 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 6661 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 6721 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 6781 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 6841 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 6901 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 6961 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 7021 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 7081 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 7141 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 7201 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 7261 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 7321 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 7381 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 7441 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 7501 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 7561 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 7621 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 7681 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 7741 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 7801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 7861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgttc 7921 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata 7981 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta 8041 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gtaactagca 8101 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagc atgcggagga 8161 aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct 8221 gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct 8281 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa 8341 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata 8401 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa 8461 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac 8521 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt 8581 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact 8641 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac 8701 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga 8761 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa 8821 cacgtccgcg atcaaatttg tagttgagct ttttggaatt gcgatcgcat aacttcgtat 8881 agcatacatt atacgaagtt ataagctcgg aacgctgcgc tcggtcgttc ggctgcggcg 8941 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 9001 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 9061 gctggcgttt ttccataggc tccgccccct gacgagcatc acaaaaatcg acgctcaagt 9121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 9181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 9241 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 9301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 9361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 9421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 9481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 9541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 9601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 9661 gatcctttgt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 9721 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 9781 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 9841 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 9901 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 9961 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 10021 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 10081 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 10141 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 10201 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 10261 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 10321 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 10381 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 10441 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 10501 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 10561 ttatcagggt tattgtctca tgtccgcgcg tt //