| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pET-Cas9-6xHis |
| 质粒类型: | CRISPR/Cas9载体；细菌基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | lac/T7 |
| 载体大小: | 9235 bp |
| 5' 测序引物及序列: | T7 forward：5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 Terminal：5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | His tag (C-ter） |
| 载体抗性: | Ampicillin |
| 克隆菌株: | Mach1 |
| 宿主细胞（系）: | 细菌，如大肠杆菌 |
| 备注: | |
| 产品目录号: | #62374 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pET-Cas9-6xHis载体质粒基本信息
pET-Cas9-6xHis质粒图谱载体图谱和pET-Cas9-6xHis载体序列质粒序列多克隆位点信息
pET-Cas9-6xHis质粒载体简介
pET-Cas9-6xHis质粒序列载体序列
LOCUS pET-Cas9-6xHis 9235 bp DNA SYN
DEFINITION pET-Cas9-6xHis
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9235
/organism="pET-Cas9-6xHis"
/mol_type="other DNA"
misc_feature 2..29
/label="lacO"
misc_feature 47..63
/label="T7_transl_en_RBS"
CDS 71..4195
/label="ORF frame 2"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDHHHHHH*"
misc_feature 4175..4192
/label="6xHIS"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDHHHHHH*"
misc_feature complement(4263..4281)
/label="T7_Terminal_primer"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDHHHHHH*"
gene complement(4931..5791)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDHHHHHH*"
CDS complement(4931..5791)
/label="ORF frame 1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5834..5862)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5949..6568
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 6967..6989
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(6983..7174)
/label="ROP"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(7746..8837)
/label="lacI"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS complement(7746..8705)
/label="ORF frame 3"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
CDS complement(8578..9078)
/label="ORF frame 2"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
gene complement(8929..9180)
/label="tet (887 - 636)"
/gene="tet (887 - 636)"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
misc_feature 9112..9131
/label="pBRrevBam_primer"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
promoter 9219..2
/label="T7_promoter"
/translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
ANCRGD*"
ORIGIN
1 GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG
61 GAGATATACC ATGGATAAGA AATACTCAAT AGGCTTAGAT ATCGGCACAA ATAGCGTCGG
121 ATGGGCGGTG ATCACTGATG AATATAAGGT TCCGTCTAAA AAGTTCAAGG TTCTGGGAAA
181 TACAGACCGC CACAGTATCA AAAAAAATCT TATAGGGGCT CTTTTATTTG ACAGTGGAGA
241 GACAGCGGAA GCGACTCGTC TCAAACGGAC AGCTCGTAGA AGGTATACAC GTCGGAAGAA
301 TCGTATTTGT TATCTACAGG AGATTTTTTC AAATGAGATG GCGAAAGTAG ATGATAGTTT
361 CTTTCATCGA CTTGAAGAGT CTTTTTTGGT GGAAGAAGAC AAGAAGCATG AACGTCATCC
421 TATTTTTGGA AATATAGTAG ATGAAGTTGC TTATCATGAG AAATATCCAA CTATCTATCA
481 TCTGCGAAAA AAATTGGTAG ATTCTACTGA TAAAGCGGAT TTGCGCTTAA TCTATTTGGC
541 CTTAGCGCAT ATGATTAAGT TTCGTGGTCA TTTTTTGATT GAGGGAGATT TAAATCCTGA
601 TAATAGTGAT GTGGACAAAC TATTTATCCA GTTGGTACAA ACCTACAATC AATTATTTGA
661 AGAAAACCCT ATTAACGCAA GTGGAGTAGA TGCTAAAGCG ATTCTTTCTG CACGATTGAG
721 TAAATCAAGA CGATTAGAAA ATCTCATTGC TCAGCTCCCC GGTGAGAAGA AAAATGGCTT
781 ATTTGGGAAT CTCATTGCTT TGTCATTGGG TTTGACCCCT AATTTTAAAT CAAATTTTGA
841 TTTGGCAGAA GATGCTAAAT TACAGCTTTC AAAAGATACT TACGATGATG ATTTAGATAA
901 TTTATTGGCG CAAATTGGAG ATCAATATGC TGATTTGTTT TTGGCAGCTA AGAATTTATC
961 AGATGCTATT TTACTTTCAG ATATCCTAAG AGTAAATACT GAAATAACTA AGGCTCCCCT
1021 ATCAGCTTCA ATGATTAAAC GCTACGATGA ACATCATCAA GACTTGACTC TTTTAAAAGC
1081 TTTAGTTCGA CAACAACTTC CAGAAAAGTA TAAAGAAATC TTTTTTGATC AATCAAAAAA
1141 CGGATATGCA GGTTATATTG ATGGGGGAGC TAGCCAAGAA GAATTTTATA AATTTATCAA
1201 ACCAATTTTA GAAAAAATGG ATGGTACTGA GGAATTATTG GTGAAACTAA ATCGTGAAGA
1261 TTTGCTGCGC AAGCAACGGA CCTTTGACAA CGGCTCTATT CCCCATCAAA TTCACTTGGG
1321 TGAGCTGCAT GCTATTTTGA GAAGACAAGA AGACTTTTAT CCATTTTTAA AAGACAATCG
1381 TGAGAAGATT GAAAAAATCT TGACTTTTCG AATTCCTTAT TATGTTGGTC CATTGGCGCG
1441 TGGCAATAGT CGTTTTGCAT GGATGACTCG GAAGTCTGAA GAAACAATTA CCCCATGGAA
1501 TTTTGAAGAA GTTGTCGATA AAGGTGCTTC AGCTCAATCA TTTATTGAAC GCATGACAAA
1561 CTTTGATAAA AATCTTCCAA ATGAAAAAGT ACTACCAAAA CATAGTTTGC TTTATGAGTA
1621 TTTTACGGTT TATAACGAAT TGACAAAGGT CAAATATGTT ACTGAAGGAA TGCGAAAACC
1681 AGCATTTCTT TCAGGTGAAC AGAAGAAAGC CATTGTTGAT TTACTCTTCA AAACAAATCG
1741 AAAAGTAACC GTTAAGCAAT TAAAAGAAGA TTATTTCAAA AAAATAGAAT GTTTTGATAG
1801 TGTTGAAATT TCAGGAGTTG AAGATAGATT TAATGCTTCA TTAGGTACCT ACCATGATTT
1861 GCTAAAAATT ATTAAAGATA AAGATTTTTT GGATAATGAA GAAAATGAAG ATATCTTAGA
1921 GGATATTGTT TTAACATTGA CCTTATTTGA AGATAGGGAG ATGATTGAGG AAAGACTTAA
1981 AACATATGCT CACCTCTTTG ATGATAAGGT GATGAAACAG CTTAAACGTC GCCGTTATAC
2041 TGGTTGGGGA CGTTTGTCTC GAAAATTGAT TAATGGTATT AGGGATAAGC AATCTGGCAA
2101 AACAATATTA GATTTTTTGA AATCAGATGG TTTTGCCAAT CGCAATTTTA TGCAGCTGAT
2161 CCATGATGAT AGTTTGACAT TTAAAGAAGA CATTCAAAAA GCACAAGTGT CTGGACAAGG
2221 CGATAGTTTA CATGAACATA TTGCAAATTT AGCTGGTAGC CCTGCTATTA AAAAAGGTAT
2281 TTTACAGACT GTAAAAGTTG TTGATGAATT GGTCAAAGTA ATGGGGCGGC ATAAGCCAGA
2341 AAATATCGTT ATTGAAATGG CACGTGAAAA TCAGACAACT CAAAAGGGCC AGAAAAATTC
2401 GCGAGAGCGT ATGAAACGAA TCGAAGAAGG TATCAAAGAA TTAGGAAGTC AGATTCTTAA
2461 AGAGCATCCT GTTGAAAATA CTCAATTGCA AAATGAAAAG CTCTATCTCT ATTATCTCCA
2521 AAATGGAAGA GACATGTATG TGGACCAAGA ATTAGATATT AATCGTTTAA GTGATTATGA
2581 TGTCGATCAC ATTGTTCCAC AAAGTTTCCT TAAAGACGAT TCAATAGACA ATAAGGTCTT
2641 AACGCGTTCT GATAAAAATC GTGGTAAATC GGATAACGTT CCAAGTGAAG AAGTAGTCAA
2701 AAAGATGAAA AACTATTGGA GACAACTTCT AAACGCCAAG TTAATCACTC AACGTAAGTT
2761 TGATAATTTA ACGAAAGCTG AACGTGGAGG TTTGAGTGAA CTTGATAAAG CTGGTTTTAT
2821 CAAACGCCAA TTGGTTGAAA CTCGCCAAAT CACTAAGCAT GTGGCACAAA TTTTGGATAG
2881 TCGCATGAAT ACTAAATACG ATGAAAATGA TAAACTTATT CGAGAGGTTA AAGTGATTAC
2941 CTTAAAATCT AAATTAGTTT CTGACTTCCG AAAAGATTTC CAATTCTATA AAGTACGTGA
3001 GATTAACAAT TACCATCATG CCCATGATGC GTATCTAAAT GCCGTCGTTG GAACTGCTTT
3061 GATTAAGAAA TATCCAAAAC TTGAATCGGA GTTTGTCTAT GGTGATTATA AAGTTTATGA
3121 TGTTCGTAAA ATGATTGCTA AGTCTGAGCA AGAAATAGGC AAAGCAACCG CAAAATATTT
3181 CTTTTACTCT AATATCATGA ACTTCTTCAA AACAGAAATT ACACTTGCAA ATGGAGAGAT
3241 TCGCAAACGC CCTCTAATCG AAACTAATGG GGAAACTGGA GAAATTGTCT GGGATAAAGG
3301 GCGAGATTTT GCCACAGTGC GCAAAGTATT GTCCATGCCC CAAGTCAATA TTGTCAAGAA
3361 AACAGAAGTA CAGACAGGCG GATTCTCCAA GGAGTCAATT TTACCAAAAA GAAATTCGGA
3421 CAAGCTTATT GCTCGTAAAA AAGACTGGGA TCCAAAAAAA TATGGTGGTT TTGATAGTCC
3481 AACGGTAGCT TATTCAGTCC TAGTGGTTGC TAAGGTGGAA AAAGGGAAAT CGAAGAAGTT
3541 AAAATCCGTT AAAGAGTTAC TAGGGATCAC AATTATGGAA AGAAGTTCCT TTGAAAAAAA
3601 TCCGATTGAC TTTTTAGAAG CTAAAGGATA TAAGGAAGTT AAAAAAGACT TAATCATTAA
3661 ACTACCTAAA TATAGTCTTT TTGAGTTAGA AAACGGTCGT AAACGGATGC TGGCTAGTGC
3721 CGGAGAATTA CAAAAAGGAA ATGAGCTGGC TCTGCCAAGC AAATATGTGA ATTTTTTATA
3781 TTTAGCTAGT CATTATGAAA AGTTGAAGGG TAGTCCAGAA GATAACGAAC AAAAACAATT
3841 GTTTGTGGAG CAGCATAAGC ATTATTTAGA TGAGATTATT GAGCAAATCA GTGAATTTTC
3901 TAAGCGTGTT ATTTTAGCAG ATGCCAATTT AGATAAAGTT CTTAGTGCAT ATAACAAACA
3961 TAGAGACAAA CCAATACGTG AACAAGCAGA AAATATTATT CATTTATTTA CGTTGACGAA
4021 TCTTGGAGCT CCCGCTGCTT TTAAATATTT TGATACAACA ATTGATCGTA AACGATATAC
4081 GTCTACAAAA GAAGTTTTAG ATGCCACTCT TATCCATCAA TCCATCACTG GTCTTTATGA
4141 AACACGCATT GATTTGAGTC AGCTAGGAGG TGACCATCAC CACCACCATC ACTAATGATA
4201 ATTTGAACGC CAGCACATGG ACTCGTCTAC TAGCGCAGCT TAATTAACCT AGGCTGCTGC
4261 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
4321 TTTGCTGAAA GGAGGAACTA TATCCGGATT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA
4381 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA
4441 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT
4501 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC
4561 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT
4621 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA
4681 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG
4741 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA
4801 TTAACGTTTA CAATTTCTGG CGGCACGATG GCATGAGATT ATCAAAAAGG ATCTTCACCT
4861 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
4921 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
4981 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
5041 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT
5101 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
5161 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
5221 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
5281 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
5341 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
5401 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
5461 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
5521 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
5581 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
5641 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
5701 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
5761 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATCA TGATTGAAGC
5821 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
5881 CAAATAGGTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
5941 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
6001 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
6061 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
6121 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
6181 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
6241 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
6301 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
6361 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
6421 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
6481 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
6541 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
6601 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
6661 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
6721 CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
6781 ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG
6841 TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC
6901 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA
6961 CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC
7021 AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT
7081 CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG
7141 CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC
7201 TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG
7261 ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC
7321 GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG
7381 GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG
7441 GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG
7501 TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG
7561 ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA
7621 GCACGATCAT GCTAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC
7681 TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG CTAACTTACA TTAATTGCGT
7741 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG
7801 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC
7861 AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG
7921 CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG
7981 ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA CCGAGATGTC CGCACCAACG
8041 CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC
8101 AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC
8161 ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT
8221 TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC
8281 GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCGTCTTCA
8341 TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA
8401 ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG
8461 ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG
8521 CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA
8581 ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC AACGCCAATC
8641 AGCAACGACT GTTTGCCCGC CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC
8701 GCCATCGCCG CTTCCACTTT TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC
8761 ACGCGGGAAA CGGTCTGATA AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT
8821 GGTTTCACAT TCACCACCCT GAATTGACTC TCTTCCGGGC GCTATCATGC CATACCGCGA
8881 AAGGTTTTGC GCCATTCGAT GGTGTCCGGG ATCTCGACGC TCTCCCTTAT GCGACTCCTG
8941 CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG
9001 TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC
9061 GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC
9121 GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC
9181 GGCGTAGAGG ATCGAGATCG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATA
//
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pET-Cas9-6xHis质粒载体应用举例
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