pcDNA-DEST40

价格：2000元

联系方式：I47-825O-882O

买家导航

pcDNA-DEST40载体质粒基本信息

出品公司:	Invitrigen
载体名称:	pcDNA-DEST40
质粒类型:	Gateway载体；哺乳细胞表达
高拷贝/低拷贝:	高拷贝
启动子:	CMV
克隆方法:	多克隆位点，限制性内切酶
载体大小:	7143bp
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	His Tag ；V5 Epitope Tag
载体抗性:	氨苄青霉素
筛选标记:	Neomycin
克隆菌株:	ccdB阳性筛选 DB3.1 感受态细胞，阴性筛选用 OmniMAX2-T1 感受态细胞
备注:	--
产品目录号:	12274-015
稳定性:	--
组成型:	组成型
病毒/非病毒:	非病毒

pcDNA-DEST40质粒图谱载体图谱和pcDNA-DEST40载体序列质粒序列多克隆位点信息

pcDNA-DEST40载体图谱

pcDNA-DEST40质粒载体简介

Gateway哺乳动物表达载体选择指南：

pDEST12.2   不带标签

pDEST26     N-His标签 G418筛选

pDEST27   N-GST标签 G418筛选

pDEST40   C-His标签、C-V5标签   G418筛选

pcDNA-DEST40   C-His标签、C-V5标签   G418筛选

pcDNA-DEST47 C-绿色荧光蛋白 G418筛选

pcDNA-DEST53 N-绿色荧光蛋白 G418筛选

pcDNA3.2/V5-DEST   C-V5标签   G418筛选

pcDNA6.2/V5-DEST   C-V5标签 Blasticidin S筛选

pcDNA6.2/N-YFP-DEST   N-黄色荧光蛋白 Blasticidin S筛选

pcDNA6.2/N-EmGFP-DEST N-绿色荧光蛋白 Blasticidin S筛选

pcDNA6.2/C-YFP-DEST C-黄色荧光蛋白 Blasticidin S筛选

pcDNA6.2/C-EmGFP-DEST C-绿色荧光蛋白 Blasticidin S筛选

pcDNA6.2/BioEase-DEST N-生物素 Blasticidin S筛选

pcDNA-DEST40质粒序列载体序列

GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTT
AAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACA
ACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCG
ATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTC
ATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG
CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCC
ATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCC
AAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTA
TGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGC
AGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA
TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACG
CAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCA
CTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGTTAAGCTATCA
ACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTT
TGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGC
ACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGGCGAGAT
TTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATG
GCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTG
GATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTC
TTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGA
TAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATAC
CACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCT
ATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTT
TGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAA
GGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCA
GAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTT
ACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATG
TATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAG
CTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAG
CCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTAT
TGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAA
AAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATG
GTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATA
TCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGA
AGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGTCAGGCTCCGTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAG
TATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTC
GTTCAGCTTTCTTGTACAAAGTGGTTGATCTAGAGGGCCCGCGGTTCGAAGGTAAGCCTATCCCTAACCC
TCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTGATCA
GCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGG
AAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCA
TTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCT
GGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACG
CGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAG
CGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAA
GCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTG
ATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTC
CACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTT
GATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACG
CGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGT
ATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAA
GTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCT
AACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAG
GCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA
AGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGAT
TGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCA
CAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTG
TCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCAC
GACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGC
GAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATG
CAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGA
GCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTC
GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATG
GCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCT
GGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAA
TGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCC
TTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGC
CATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGC
CGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCA
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATT
CTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAG
CTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATA
CGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGC
GCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG
GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT
CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC
TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAA
CTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGA
GTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA
CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT
TAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAA
TCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTA
GATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCA
CCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTT
TATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT
GCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGC
TCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCG
GTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAA
TTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGA
GAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCA
GAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTT
GAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTT
TCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAA
TACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACAT
ATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGAC
GTC

pLexm	pHSG422
pLLP-OmpA	pDEST26
pCGN978	pET-21a(+)
pET-28a-BFP-C	pMal-c4X
pML21	pTP-4
pBBR1MCS-2	pMDLg/pRRE
pDsRed-Monomer	pEF1/V5-HisA
pIJ702-del35-55	pET-41a(+)
pWF1	pLNCX2
c-Flag pcDNA3	pAD-SV40T
pOrf681_TA	pCL-Ampho
pBluescript II KS(-)	pHS85
pET-12c(+)	pIκB-EGFP
pFA1	pHB-1
pFLAG-CMV-3	pTet-Splice
pdKeima-Red-S1	pcDNA6.2-DsRed2-MCS1 miR
pENTR11-Dual	ISceI-GR-RFP
pMTD1.3xA	pGL4.41[luc2P/HSE/Hygro]
pEF6/myc-His B	pDC515
pGL4.38[luc2P/p53 RE/Hygro]	pJSC73
pBT-4	pUG6
pLVX-DD-ZsGreen1 Control	pSUPER.neo+gfp
pNEO-ars	pSC101
pPIC9k-His	pREP4
pCDH-CMV-MCS-EF1a-RFP+BSD	pACT-MyoD
pRSET-EGFP	pMal-c2G
pcDNA3.1(+)/CAT	pFLAG-CMV2
pVPack-GP	pGL4.45[luc2P/ISRE/Hygro]
pCRE-DD-AmCyan1	pIC20H
pL-brevisGH3-MBP	pSC101-EcoRImeth+
pRB373	pHY15
pATPase_TA	pPIC ZαGB
pSP72	pUCTag
pALEX a,b,c	pGL4.72[hRlucCP]
pBD-NF-kB	pET-32a(+)
pSH65	pET-32 EK/LIC
pSET152	pNEO
PX543	pCH2
pOM2	pBAD18
pCas9D10A-GFP	pcDNA4/HisMax B
pPIC9	pTi
pKLAC1	pSUMO
pAN7-1	pMT-Bip-V5-HisA
pcDNA5/FRT/V5-His-TOPO	pProLabel-C
pAGO	pBad43
pUC4-KISS	pHV23
pcDNA5/FRT	pCRE-MetLuc2-Reporter
Pgex-HRV3C	pcDNA3.1-GP130v1
pCREj-2	pAcGFP1-N2
EWD299	pCYM1

pCMV-hA3G-BE3	EC2_10_dCas9_VPR_HC_sgRNA
PB_tre_Cas9	pCAG-GBP1-10gly-lexADBD
pNEU-hMbPylRS-4xU6M15	NM9-SpCas9-NLS3
pBHt2	phage UbiC NLS HA stdMCP stdHalo
pT4-CMV-GFP	pQUAST>stop>mCD8-GFP
pICH86966::AtU6p::sgRNA_PDS	pAC1404-pCR8-mRuby2_NLSPUFa
pAAVS1-PDi-CRISPRn	CKS2 gRNA (BRDN0001149143)
pSR47	pAAV-EFS-CjCas9-eGFP-HIF1a
Lenti-luciferase-P2A-Neo	800 pSG5L HA PTEN wt
pNeae2	pcDNA3.4_7C11-1 heavy chain
pPPEM	pET28a-At-TRL
BECLIN-HA WT	pAAV2/8
EC1000	pAraGFPCDF
AbVec1.1-IGKC	pNK9
pSELECT-HA-mFOXO1	pMJ329: pCMV-MmeFz2
pLVX-M-puro	pLenti-EML4-ALK variant 3a
pAAV-EF1a-FLEX-GTB	pCXLE-hOCT3/4
pNN9	3XMEF2-luc
pUCC001	pTEF:ATP
pCS2TAL3-DD	pSBtet-GP
pDIRECT_23D	Cbh_v5 AAV-CBE N-terminal
pCS2TAL3-RR	pXAT2
GST-HA-GFP11-N1	pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I
pMSCV-IRES-GFP II (pMIG II)	pAc-Neo-OVA
pMonAID_nCas9-PmCDA_Hyg_ALS	p58sheLL
pAAVS1-PC-GCaMP6f	pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV
pCMV-MMLVgag-3xNES-ABE8e	LeGO-G
pLJC5-3XHA-EGFP-PEX26	PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a
EC2_3_dCas9_Mxi1_sgRNA	pM1s3AsR_TS-C7
LB1366 (Bcl-2 promoter with MHS1234)	pRAD1
pCDH-puro-Bcl-XL	pFC334
pTRKH2	pMal-T-Avi-His/BirA
pC003 - LshC2C2 locus into pACYC184 for	401 pSG5L
p201G Cas9	MSCV-(pBabe mcs)- human p210BCR-ABL-IRES
VVT1	pCAS9-mCherry empty
pHAGE-PTCH1	1306 pSG5L HA FKHR wt
pJKR-L-tetR	TAL3393
pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK	pYES-mtGFP
pLentiV2-dCas9-VP64	pTRE Tight ArchT-GFP
pGL3 2 kb prom. CD274	ilux pGEX(-)
pEGFP-puro	pEGFP-LC3
pMSCV-hygro-STING	pMXs-hOCT4 (Plath)
pRC-CMV-Rev1b	Tet-O-FUW-Neurod1
pLenti-DsRed_IRES_MAPT:EGFP	pT3-Neo-EF1a-GFP
c-myc-PT3EF1a	pLenti X2 Puro DEST (w16-1)
pJSC131 - Bacterial expression plasmid f	pCW-Cas9
BPK3082	pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)	pM-Cas9
FUmGW	pAc-sgRNA-Cas9
pGH335_MS2-AID*Δ-Hygro	pGGA008

热点推荐

生物资源

细胞培养

微生物与植物培养

蛋白抗体与激酶

技术服务

课题设计与论文发表

pcDNA-DEST40

买家导航

pcDNA-DEST40载体质粒基本信息

pcDNA-DEST40质粒图谱载体图谱和pcDNA-DEST40载体序列质粒序列多克隆位点信息

pcDNA-DEST40质粒载体简介

pcDNA-DEST40质粒序列载体序列

pcDNA-DEST40载体图谱质粒图谱pdf版和pcDNA-DEST40载体序列质粒序列等相关资料下载

pcDNA-DEST40质粒载体应用举例

全部质粒载体分类

相关质粒载体

相关质粒载体文章

相关载体质粒问答