| 出品公司: | INVITROGEN |
|---|---|
| 载体名称: | pCMV-Red-Firefly-Luc ; pCMV-Red Firefly Luc |
| 质粒类型: | 哺乳动物载体；红色荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6575 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | Red Firefly Luciferase红色萤火虫荧光素酶 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | 常规哺乳动物细胞 |
| 备注: | -- |
| 产品目录号: | 16156 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-Red-Firefly-Luc载体质粒基本信息
pCMV-Red-Firefly-Luc质粒图谱载体图谱和pCMV-Red-Firefly-Luc载体序列质粒序列多克隆位点信息
pCMV-Red-Firefly-Luc质粒载体简介
pCMV-Red Firefly Luc载体说明
Thermo Scientific pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter for co-transfection and normalization control to account for experimental variation in combination with other reporters in a gene regulation study using the intracellular red firefly luciferase reporter with excellent light intensity..
Features of Red Firefly Luc:
• Red Firefly Luc Vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
• pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
• pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively.
• These pCMV and pTK constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.
These vectors are subject to a limited use label license.
More Product Data
• Highly sensitive multiplex luciferase reporter assays
• Monitoring neuronal differentiation using multiplexed luciferase reporters
• Activation of the antioxidant response pathway by pesticide chemicals
pCMV-Red-Firefly-Luc质粒序列载体序列
LOCUS pCMV-Red Firefly Luc 6575 bp DNA SYN
DEFINITION pCMV-Red Firefly Luc
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6575
/organism="pCMV-Red Firefly Luc"
/mol_type="other DNA"
promoter 13..605
/label="CMV_immearly_promoter"
misc_feature 68..355
/label="CAG_enhancer"
misc_feature 522..542
/label="CMV_fwd_primer"
CDS 527..2293
/label="ORF frame 2"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
promoter 616..634
/label="T7_promoter"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
misc_feature complement(2313..2330)
/label="BGH_rev_primer"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
misc_feature complement(2548..2568)
/label="pBABE_3_primer"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
misc_feature complement(2554..2769)
/label="SV40_enhancer"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
promoter 2566..2834
/label="SV40_promoter"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
rep_origin 2733..2810
/label="SV40_origin"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
misc_feature 2795..2814
/label="SV40pro_F_primer"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
promoter 2928..2995
/label="EM7_promoter"
/translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
GLTGKIDGRAIREILKKPVAKM*"
CDS 2996..3595
/label="ORF frame 2"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
misc_feature 3816..3835
/label="EBV_rev_primer"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
gene complement(3899..4759)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
CDS complement(3899..4759)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4801..4829)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5007..5626
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 5881..5897
/label="M13_forward20_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 6397..605
/label="CMV_immearly_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 6524..6543
/label="RVprimer3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 AACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
361 TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
421 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA
481 ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG
541 TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA
601 CTGGCTTATC GAAATTAATA CGACTCACTA TAGGGGATCC GCCACCATGG AAAATATGGA
661 AAACGACGAG AACATCGTGG TGGGCCCCAA GCCCTTCTAC CCCATCGAGG AAGGCAGCGC
721 CGGCACCCAG CTGCGGAAGT ACATGGAAAG ATACGCCAAG CTGGGCGCCA TTGCCTTCAC
781 CAACGCCGTG ACCGGCGTGG ACTACAGCTA CGCCGAGTAC CTGGAAAAGA GCTGCTGCCT
841 GGGCAAGGCT CTGCAGAACT ACGGCCTGGT GGTGGACGGC CGGATCGCCC TGTGCAGCGA
901 GAACTGCGAG GAATTCTTCA TCCCCGTGAT CGCCGGCCTG TTCATCGGCG TGGGCGTGGC
961 TCCCACCAAC GAGATCTACA CCCTGCGGGA GCTGGTGCAC AGCCTGGGCA TCAGCAAGCC
1021 CACCATCGTG TTCAGCAGCA AGAAGGGCCT GGACAAAGTC ATCACCGTGC AGAAAACCGT
1081 GACCACCATC AAGACCATCG TGATCCTGGA CAGCAAGGTG GACTACCGGG GCTACCAGTG
1141 CCTGGACACC TTCATCAAGC GGAACACCCC CCCTGGCTTC CAGGCCAGCA GCTTCAAGAC
1201 CGTGGAGGTG GACCGGAAAG AACAGGTGGC CCTGATCATG AACAGCAGCG GCAGCACCGG
1261 CCTGCCCAAG GGCGTGCAGC TGACCCACGA GAACACCGTG ACCCGGTTCA GCCACGCCAG
1321 GGACCCCATC TACGGCAACC AGGTGTCCCC CGGCACCGCC GTGCTGACCG TGGTGCCCTT
1381 CCACCACGGC TTCGGCATGT TCACCACCCT GGGCTACCTG ATCTGCGGCT TCCGGGTGGT
1441 GATGCTGACC AAGTTCGACG AGGAAACCTT CCTGAAAACC CTGCAGGACT ACAAGTGCAC
1501 CTACGTGATT CTGGTGCCCA CCCTGTTCGC CATCCTGAAC AAGAGCGAGC TGCTGAACAA
1561 GTACGACCTG AGCAACCTGG TGGAGATCGC CAGCGGCGGA GCCCCCCTGA GCAAAGAAGT
1621 GGGAGAGGCC GTCGCCAGGC GGTTCAATCT GCCCGGCGTG CGGCAGGGCT ACGGCCTGAC
1681 CGAGACAACC AGCGCCATCA TCATCACCCC CGAGGGCGAC GACAAGCCTG GAGCCAGCGG
1741 CAAGGTGGTG CCCCTGTTCA AGGCCAAAGT GATCGACCTG GACACCAAGA AGAGCCTGGG
1801 CCCCAACAGA CGGGGCGAAG TGTGCGTGAA GGGCCCCATG CTGATGAAGG GCTACGTGAA
1861 CAACCCCGAG GCCACCAAAG AGCTGATCGA CGAAGAGGGC TGGCTGCACA CCGGCGACAT
1921 CGGCTACTAC GACGAAGAGA AGCACTTCTT CATCGTGGAC CGGCTGAAGA GCCTGATCAA
1981 GTACAAGGGC TATCAGGTGC CCCCTGCCGA GCTGGAAAGC GTCCTGCTGC AGCACCCCAG
2041 CATCTTCGAC GCCGGCGTGG CCGGGGTGCC AGATCCTGTG GCCGGCGAGC TGCCTGGCGC
2101 CGTGGTGGTG CTGGAATCCG GCAAGAACAT GACCGAGAAA GAAGTGATGG ACTACGTCGC
2161 CAGCCAGGTG TCCAACGCCA AGCGGCTGAG AGGCGGCGTG AGATTCGTGG ACGAAGTGCC
2221 AAAGGGCCTG ACCGGCAAGA TCGACGGCAG GGCCATCCGG GAGATCCTGA AGAAACCCGT
2281 GGCCAAGATG TGATGAGCGG CCGCAAAATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC
2341 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT
2401 CCTTTCCTAA TAAAATGAGG AAATTGCATC ACAACACTCA ACCCTATCTC GGTCTATTCT
2461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
2521 CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC
2581 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT
2641 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT
2701 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG
2761 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT
2821 CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
2881 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
2941 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGAC
3001 CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC
3061 CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA
3121 CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG
3181 CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT
3241 CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG
3301 GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC
3361 GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC
3421 CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC
3481 ATCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT
3541 CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACACGT
3601 GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT
3661 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA
3721 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
3781 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
3841 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATT
3901 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
3961 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
4021 GCGCTGCGAT GATACCGCGA GAACCACGCT CACCGGCTCC GGATTTATCA GCAATAAACC
4081 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
4141 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
4201 TTGTTGCCAT CGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
4261 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
4321 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
4381 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
4441 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
4501 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
4561 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
4621 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
4681 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
4741 GGAAATGTTG AATACTCATA TTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
4801 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCA
4861 GTGTTACAAC CAATTAACCA ATTCTGAACA TTATCGCGAG CCCATTTATA CCTGAATATG
4921 GCTCATAACA CCCCTTGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC GCGCGTCGTT
4981 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
5041 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
5101 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
5161 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGCCCACCAC TTCAAGAACT CTGTAGCACC
5221 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
5281 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
5341 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
5401 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
5461 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
5521 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
5581 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
5641 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT
5701 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA
5761 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGGCGAGAGT AGGGAACTGC CAGGCATCAA
5821 ACTAAGCAGA AGGCCCCTGA CGGATGGCCT TTTTGCGTTT CTACAAACTC TTTCTGTGTT
5881 GTAAAACGAC GGCCAGTCTT AAGCTCGGGC CCCCTGGGCG GTTCTGATAA CGAGTAATCG
5941 TTAATCCGCA AATAACGTAA AAACCCGCTT CGGCGGGTTT TTTTATGGGG GGAGTTTAGG
6001 GAAAGAGCAT TTGTCAGAAT ATTTAAGGGC GCCTGTCACT TTGCTTGATA TATGAGAATT
6061 ATTTAACCTT ATAAATGAGA AAAAAGCAAC GCACTTTAAA TAAGATACGT TGCTTTTTCG
6121 ATTGATGAAC ACCTATAATT AAACTATTCA TCTATTATTT ATGATTTTTT GTATATACAA
6181 TATTTCTAGT TTGTTAAAGA GAATTAAGAA AATAAATCTC GAAAATAATA AAGGGAAAAT
6241 CAGTTTTTGA TATCAAAATT ATACATGTCA ACGATAATAC AAAATATAAT ACAAACTATA
6301 AGATGTTATC AGTATTTATT ATCATTTAGA ATAAATTTTG TGTCGCCCTT AATTGTGAGC
6361 GGATAACAAT TACGAGCTTC ATGCACAGTG GCGTTGACAT TGATTATTGA CTAGCATGTT
6421 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCATGC ATTAGTTATT
6481 AATAACATAC GCTCTCCATC AAAACAAAAC GAAACAAAAC AAACTAGCAA AATAGGCTGT
6541 CCCCAGTGCA AGTGCAGGTG CCAGAACATT TCTCT
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