| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pCMV-SPORT1 |
| 质粒类型: | 哺乳动物表达载体；cDNA克隆载体；I.M.A.G.E.载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4380 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | 用于cDNA文库的构建 |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-SPORT1载体质粒基本信息
pCMV-SPORT1质粒图谱载体图谱和pCMV-SPORT1载体序列质粒序列多克隆位点信息
pCMV-SPORT1质粒载体简介
pCMV-SPORT1质粒序列载体序列
LOCUS Exported 4380 bp ds-DNA circular SYN 28-FEB-2013
DEFINITION Vector for cloning and transient mammalian cell expression of cDNAs.
ACCESSION .
VERSION .
KEYWORDS pCMV SPORT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4380)
AUTHORS I.M.A.G.E. Consortium
FEATURES Location/Qualifiers
source 1..4380
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
polyA_signal 125..259
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 646..664
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 682..779
/note="MCS"
/note="multiple cloning site"
promoter complement(787..805)
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter complement(921..1124)
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1125..1428
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
protein_bind 1458..1474
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1482..1513)
/note="lac UV5 promoter"
/note="E. coli lac promoter with an ""up"" mutation"
rep_origin complement(1975..2563)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2641..2674
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(2776..3636)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin complement(3924..4379)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcttataata cgactcacta
661 tagggagaga gctatgacgt cgcatgcacg cgtaagcttg ggcccctcga gggatcctct
721 agagcggccg ccgactagtg agctcgtcga cccgggaatt ccggaccggt acctgcaggc
781 gtaccttcta tagtgtcacc taaatagctt tttgcaaaag cctaggctag agtccggagg
841 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
901 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
961 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
1021 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
1081 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
1141 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
1201 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
1261 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
1321 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
1381 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgac ctgcacgatg
1441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggaagc
1501 tataaagtgt aaagcctggg gtgcctaatg agtgaaaggg cctcgtatac gcctattttt
1561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
1621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
1681 gagacaataa ccctgataaa tgcttcaata atattgaaaa acgcgcgaat tgcaagctct
1741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
1801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
1861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
2041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
2101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
2161 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
2221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
2341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
2401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
2461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
2521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
2581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc
2641 ataacttcgt atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt
2701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
2761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
2821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
2881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
2941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
3001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
3061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
3121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
3181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
3241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
3301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
3361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
3421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
3481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
3541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
3601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
3661 aagcatttat cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg
3721 atccctacac agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat
3781 tctccctcca gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg
3841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
3901 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt
3961 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
4021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
4081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
4141 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
4201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
4261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
4321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
//
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