| 出品公司: | Clontech |
|---|---|
| 载体名称: | pEF1α-tdTomato |
| 质粒类型: | 哺乳动物细胞表达载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | EF1α |
| 载体大小: | 6149 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 新霉素（Neomycin） |
| 克隆菌株: | DH5α, HB101 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
pEF1α-tdTomato载体组成型表达tdTomato； tdTomato基因片段上下游有酶切位点，很容易克隆到其它载体上去。 |
| 产品目录号: | 631975 |
| 稳定性: | 稳表达 或 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pEF1α-tdTomato载体质粒基本信息
pEF1α-tdTomato质粒图谱载体图谱和pEF1α-tdTomato载体序列质粒序列多克隆位点信息
pEF1α-tdTomato质粒载体简介
pEF1α-tdTomato质粒序列载体序列
LOCUS pEF1a-tdTomato 6149 bp DNA circular 17-MAR-2010
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631975
FEATURES Location/Qualifiers
misc_feature 4045..4839
/label=Kan(R)/Neo(R)
CDS 1385..2812
/label=tdTomato
promoter 12..1346
/label=EF1a\Promoter
polyA_signal 2968..3002
/label=SV40\polyA\signal
rep_origin complement(3065..3520)
/label=f1\origin
rep_origin 3861..3999
/label=SV40\origin
polyA_signal 5075..5093
/label=HSV\TK\polyA\signals
rep_origin 5424..6067
/label=pUC\origin
misc_feature 1378..1388
/label=Kozak\sequence
misc_feature 3694..3962
/label=SV40\early\Promoter
BASE COUNT 1352 a 1725 c 1751 g 1321 t
ORIGIN
1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga
61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc
121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca
181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc
481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg
601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc caccggtcgc
1381 caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
1441 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
1501 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
1561 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
1621 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
1681 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
1741 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
1801 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
1861 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
1921 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
1981 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
2041 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
2101 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
2161 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
2221 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
2281 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
2341 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
2401 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
2461 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
2521 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
2581 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
2641 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
2701 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
2761 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaggcggc
2821 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
2881 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
2941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
3001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
3061 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
3121 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
3181 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
3241 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
3301 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
3361 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
3421 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
3481 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
3541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
3601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
3661 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
3961 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
4021 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
4081 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
4141 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
4201 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
4261 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
4321 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
4381 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
4441 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
4501 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
4561 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
4621 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
4681 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
4741 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
4801 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
4861 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
4921 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
4981 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
5041 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
5101 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
5161 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
5221 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
5281 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
5341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
5401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
5461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
5521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
5581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
5641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
5701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
5761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
5821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
5881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
5941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
6001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
6061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
6121 ctgtggataa ccgtattacc gccatgcat
//
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pEF1α-tdTomato质粒载体应用举例
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