| 出品公司: | Takara |
|---|---|
| 载体名称: | pGro7 |
| 质粒类型: | Chaperone Plasmid分子伴侣质粒 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5.4kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | chloromycetin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
pGro7载体质粒基本信息
pGro7质粒图谱载体图谱和pGro7载体序列质粒序列多克隆位点信息
pGro7质粒载体简介
pGro7质粒序列载体序列
LOCUS pGro7 5467 bp ds-DNA circular SYN 10-JUN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGro7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5467)
AUTHORS caoheibi
TITLE Direct Submission
JOURNAL Exported Friday, Jun 10, 2022 from SnapGene 3.2.1
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5467
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 530..1766
/note="araC"
CDS complement(576..1421)
/codon_start=1
/note="araC"
/note="arabinose operon transcriptional regulator AraC"
/translation="MAETQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGEGVINNNGEQFVCRPGDILLFPPGEIHHYGRHPDASEWYHQWVYFRPRAYW
QEWLTWPTIFAQTGFFRPDEARQPHFSELFGQIISAGQGEGRYSELLAINLLEQLLLRR
MAVINESLHPPMDSRVRDACQYISDHLADSHFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
"
CDS 1817..2110
/codon_start=1
/note="GroES"
/note="co-chaperone GroES"
/translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGN
GRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
CDS 1862..1915
/codon_start=1
/product="GroES chaperone mobile loop that interacts with
GroEL"
/note="S loop"
/translation="EVETKSAGGIVLTGSAAA"
CDS 2154..3800
/codon_start=1
/note="groEL"
/note="chaperonin GroEL"
/translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFG
APTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGL
KAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIA
EAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILL
ADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGF
GDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEA
AIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDA
LHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNC
GEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITT
ECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
rep_origin 4076..4620
/direction=RIGHT
/note="p15A ori"
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
promoter 5146..5248
/note="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 5249..441
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaag aatagttacg
541 gcttatgaca tctttgtgga cacatcattc actttttatt cacatccggc cctgaactcg
601 ctaggacttg ccccggtgca ttttttaaat acccgcgaaa aatagagctg atcgtcaaat
661 ccaacattgc gcccaacggt cgctatcggc attcgcgtag tgctaagcag aagtttcgcc
721 tggctgatac gctgatcttc gcgccagctc aatacgctaa tgcctaactg ctggcggaac
781 agatgtgata accgggaggg cgacaggcag acatgctggg cgacgctggc gatatcaaaa
841 tggctgtccg ccagatggtc gctgatatac tggcaggcat cgcgcacacg gctatccatc
901 ggcgggtgca acgactcatt aattaccgcc atacgtctga gcaacaactg ctccagcaga
961 ttgatcgcca gtagctcaga atagcgacct tccccttgcc cggcgctgat gatctgcccg
1021 aacagttcgc tgaaatgcgg ctggcgcgcc tcgtccgggc ggaaaaatcc tgtctgggca
1081 aagattgtcg gccaggtcag ccactcctgc cagtaggcgc gaggccggaa ataaacccac
1141 tggtgatacc actcgctggc gtccggatgc cgtccatagt gatgaatctc gcccggcgga
1201 aacaataata tatcgccagg ccgacagaca aactgctcgc cattattatt aatgacgccc
1261 tctccgcgga tggtcaggtt aagaatatat cccttcatgc ccaacggacg atcgataaaa
1321 aaatccagat atccattcgc ttcaattggc gtcagcccgg cgaccagatg ggcattaaat
1381 gaatatcccg gcaatagcgg atcattttgc gtttcagcca tgatttctct accccccgat
1441 gttcagagaa gaaacaaatt gtccatatcg accaggacga cagagcttcc gtctccgcaa
1501 gactttgcgc ttgatgaaag cacgtatcaa ccccgcttgt gaaaagcgct ttgtaacaaa
1561 agcgtacagt tcaggcgata aaattaagta acagaagtgt ctataactat ggctggaatg
1621 tccacattga atatttgcac agcgtcacac tttgcaaagc attagcattt ttgtccataa
1681 gattagcgga tcctgcctga cggtttttgc cgcgactctc tataatttct ccatacctgt
1741 ttttctggat ggagtaagac catggctcga ggcgtcaccc ataacagata cggactttct
1801 caaaggagag ttatcaatga atattcgtcc attgcatgat cgcgtgatcg tcaagcgtaa
1861 agaagttgaa actaaatctg ctggcggcat cgttctgacc ggctctgcag cggctaaatc
1921 cacccgcggc gaagtgctgg ctgtcggcaa tggccgtatc cttgaaaatg gcgaagtgaa
1981 gccgctggat gtgaaagttg gcgacatcgt tattttcaac gatggctacg gtgtgaaatc
2041 tgagaagatc gacaatgaag aagtgttgat catgtccgaa agcgacattc tggcaattgt
2101 tgaagcgtaa tccgcgcacg acactgaaca tacgaattta aggaataaag ataatggcag
2161 ctaaagacgt aaaattcggt aacgacgctc gtgtgaaaat gctgcgcggc gtaaacgtac
2221 tggcagatgc agtgaaagtt accctcggtc caaaaggccg taacgtagtt ctggataaat
2281 ctttcggtgc accgaccatc accaaagatg gtgtttccgt tgctcgtgaa atcgaactgg
2341 aagacaagtt cgaaaatatg ggtgcgcaga tggtgaaaga agttgcctct aaagcaaacg
2401 acgctgcagg cgacggtacc accactgcaa ccgtactggc tcaggctatc atcactgaag
2461 gtctgaaagc tgttgctgcg ggcatgaacc cgatggacct gaaacgtggt atcgacaaag
2521 cggttaccgc tgcagttgaa gaactgaaag cgctgtccgt accatgctct gactctaaag
2581 cgattgctca ggttggtacc atctccgcta actccgacga aaccgtaggt aaactgatcg
2641 ctgaagcgat ggacaaagtc ggtaaagaag gcgttatcac cgttgaagac ggtaccggtc
2701 tgcaggacga actggacgtg gttgaaggta tgcagttcga ccgtggctac ctgtctcctt
2761 acttcatcaa caagccggaa actggcgcag tagaactgga aagcccgttc atcctgctgg
2821 ctgacaagaa aatctccaac atccgcgaaa tgctgccggt tctggaagct gttgccaaag
2881 caggcaaacc gctgctgatc atcgctgaag atgtagaagg cgaagcgctg gcaactctgg
2941 ttgttaacac catgcgtggc atcgtgaaag tcgctgcggt taaagcaccg ggcttcggcg
3001 atcgtcgtaa agctatgctg caggatatcg caaccctgac tggcggtacc gtgatctctg
3061 aagagatcgg tatggagctg gaaaaagcaa ccctggaaga cctgggtcag gctaaacgtg
3121 ttgtgatcaa caaagacacc accactatca tcgatggcgt gggtgaagaa gctgcaatcc
3181 agggccgtgt tgctcagatc cgtcagcaga ttgaagaagc aacttctgac tacgaccgtg
3241 aaaaactgca ggaacgcgta gcgaaactgg caggcggcgt tgcagttatc aaagtgggtg
3301 ctgctaccga agttgaaatg aaagagaaaa aagcacgcgt tgaagatgcc ctgcacgcga
3361 cccgtgctgc ggtagaagaa ggcgtggttg ctggtggtgg tgttgcgctg atccgcgtag
3421 cgtctaaact ggctgacctg cgtggtcaga acgaagacca gaacgtgggt atcaaagttg
3481 cactgcgtgc aatggaagct ccgctgcgtc agatcgtatt gaactgcggc gaagaaccgt
3541 ctgttgttgc taacaccgtt aaaggcggcg acggcaacta cggttacaac gcagcaaccg
3601 aagaatacgg caacatgatc gacatgggta tcctggatcc aaccaaagta actcgttctg
3661 ctctgcagta cgcagcttct gtggctggcc tgatgatcac caccgaatgc atggttaccg
3721 acctgccgaa aaacgatgca gctgacttag gcgctgctgg cggtatgggc ggcatgggtg
3781 gcatgggcgg catgatgtaa ttgccctgca cctcgcagaa ataaacaaac ccccgggcag
3841 aaatgtctgg gggtttttct tttggtcatc tttctagtat aagattcaga cacggacgac
3901 gcgagtggcg cctcgaggcg ccgatatcag atctggtacc aagcttatcg atgataagct
3961 gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa
4021 gagataaatt gcactgaaat ctagaaatat ttatctgatt aataagatga tcttcttgag
4081 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
4141 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
4201 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
4261 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
4321 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
4381 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
4441 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
4501 gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
4561 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
4621 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
4681 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
4741 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
4801 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
4861 gtaagccagt atacactccg ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc
4921 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
4981 caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccgacg cactttgcgc
5041 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
5101 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
5161 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
5221 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
5281 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
5341 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
5401 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
5461 ggaattc
//
