| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET43.1c, pET 43.1c |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7230 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3' 测序引物: | ColiDOWN |
| 3' 测序引物序列: | ColiDOWN: 5'-TTCACTTCTGAGTTCGGCATG-3' |
| 载体标签: | C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK |
| 载体抗性: | Ampicillin |
| 备注: |
C term His tag only; N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS
|
| 产品目录号: | 70941-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)、C43(DE3) |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-43.1c(+)载体 pET43.1c质粒基本信息
pET-43.1c(+)质粒图谱 pET43.1c载体图谱和pET-43.1c(+)载体序列 pET43.1c质粒序列多克隆位点信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_Terminal_primer | 69 | 87 | |
| rrnB_T2_terminator | 168 | 141 | |
| rrnB_T1_terminator | 343 | 300 | |
| rrnB_terminator | 466 | 309 | |
| EK | 693 | 679 | |
| S15 | 789 | 745 | |
| 6xHIS | 816 | 799 | |
| T7_transl_en_RBS | 2341 | 2325 | |
| lacO | 2386 | 2359 | |
| T7_promoter | 2404 | 2386 | |
| tet (300 - 563) | 2440 | 2703 | |
| pBRrevBam_primer | 2511 | 2492 | |
| lacI | 2786 | 3877 | |
| ROP | 4449 | 4640 | |
| pGEX_3_primer | 4656 | 4634 | |
| pBR322_origin | 5674 | 5055 | |
| AmpR_promoter | 5761 | 5789 | |
| Ampicillin | 5832 | 6692 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 427 | 2130 | |
| ORF frame 1 | 2316 | 499 | |
| ORF frame 1 | 2317 | 3045 | |
| ORF frame 2 | 2918 | 3877 | |
| ORF frame 3 | 5832 | 6692 |
| Enzyme Name | Cut |
|---|---|
| PacI | 503 |
| XhoI | 530 |
| PmlI | 576 |
| NotI | 592 |
| EagI | 592 |
| HindIII | 599 |
| KpnI | 624 |
| SalI | 626 |
| PstI | 639 |
| AscI | 641 |
| EcoRI | 654 |
| BamHI | 660 |
| SacI | 672 |
| SmaI | 719 |
| XmaI | 717 |
| SacII | 795 |
| SpeI | 826 |
| BglII | 941 |
| MscI | 1073 |
| StuI | 1126 |
| NcoI | 1401 |
| NdeI | 2315 |
| XbaI | 2353 |
| ApaI | 3356 |
| HpaI | 3651 |
| FspI | 6396 |
pET-43.1c(+)质粒 pET43.1c载体简介
pET-43.1系列载体是带有Nus蛋白标签，可以在大肠杆菌中高表达目的蛋白。本载体带有N-Trx标签和His标签，S标签。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET-43.1c(+)质粒序列 pET43.1c载体序列
LOCUS pET-43.1c(+) 7230 bp DNA Circular SYN
DEFINITION pET-43.1c(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..7230
/organism="pET-43.1 c (+)"
/mol_type="other DNA"
misc_feature 69..87
/label="T7_Terminal_primer"
terminator complement(141..168)
/label="rrnB_T2_terminator"
terminator complement(300..343)
/label="rrnB_T1_terminator"
terminator complement(309..466)
/label="rrnB_terminator"
CDS 427..2130
/label="ORF frame 1"
CDS complement(499..2316)
/label="ORF frame 1"
misc_feature complement(679..693)
/label="EK"
misc_feature complement(745..789)
/label="S15"
misc_feature complement(799..816)
/label="6xHIS"
CDS 2317..3045
/label="ORF frame 1"
misc_feature complement(2325..2341)
/label="T7_transl_en_RBS"
misc_feature complement(2359..2386)
/label="lacO"
promoter complement(2386..2404)
/label="T7_promoter"
gene 2440..2703
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(2492..2511)
/label="pBRrevBam_primer"
misc_feature 2786..3877
/label="lacI"
CDS 2918..3877
/label="ORF frame 2"
misc_feature 4449..4640
/label="ROP"
misc_feature complement(4634..4656)
/label="pGEX_3_primer"
rep_origin complement(5055..5674)
/label="pBR322_origin"
promoter 5761..5789
/label="AmpR_promoter"
gene 5832..6692
/label="Ampicillin"
/gene="Ampicillin"
CDS 5832..6692
/label="ORF frame 3"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggata catatttgaa tgtatttaga aaaataaaca
121 aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttaatttgat
181 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcgcaacgt
241 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
301 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc
361 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
421 ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc
481 agcctaggaa cgcccaactt aattaacatt agtggtggtg gtggtggtgc tcgagatcct
541 ctgggtcttc aggagcgagt tctggctggc ttgcacgtgt atacagctgt gcggccgcaa
601 gcttcgaacg cgtatcgatg gtaccgtcga cgtcctgcag gcgcgcctgt acagaattcg
661 gatccacgag ctcggactct tgtcgtcgtc atcaatcgta ccagaacccg cgctgccccg
721 ggggaccaga ccagttggcg gtggcgagtc catgtgctgg cgttcaaatt tcgcagcagc
781 ggtttcttta cccgcggagt gatggtgatg gtgatgacca gaaccactag tcgcttcgtc
841 accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa
901 cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac
961 gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc
1021 gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc
1081 atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa
1141 cagctctttc atcggcacat aggccaattc ttccagcgtc gagaagcctt cttctaccag
1201 aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg
1261 cgcttccgcc tgatgcttag cttgcaggtc gtcaacggtc atcacgttga gttcccaacc
1321 gctcagttgc gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccagatt
1381 accggcttca acggcgatgt ccatggtgtg tttatcttca tccaccacga tagaagcaac
1441 gtctgccggt gccattgcgt taatcacgaa ctgcgccggg ttatcatccc acaggacgat
1501 atcgatacgc tcgccaccca gttcagtaga caccgcctga acacgcgcgc cacgcatacc
1561 tacgcaagca cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg
1621 agaacccgga tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttctggcac
1681 ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc
1741 gccacgcgct tccgggcgaa cggaatagag cacgccacga acgcggtcgc cagggcggaa
1801 gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc
1861 cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg
1921 ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac
1981 ctgttttgcc gtctgggtag tgatacggtc aaaggtaaca gactcaatct gatcttcaac
2041 gtaatcgccc aggttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt
2101 ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaagtgtcaa aatcaccgct
2161 tttgcgatcg atctgtacgc ggacgtcgat ctcttgttca tattttttct ttgttgctgt
2221 cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga
2281 tacggcttca actacagcca aaatttcttt gttcatatgt atatctcctt cttaaagtta
2341 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
2401 attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc
2461 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
2521 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
2581 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
2641 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
2701 gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata
2761 gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt
2821 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
2881 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
2941 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
3001 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
3061 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
3121 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
3181 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
3241 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
3301 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
3361 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
3421 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
3481 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
3541 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggacatctc
3601 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
3661 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
3721 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
3781 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
3841 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
3901 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
3961 gctccttccg gtgggcgcgg ggcatgacta gcatgatcgt gctcctgtcg ttgaggaccc
4021 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
4081 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
4141 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
4201 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
4261 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
4321 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
4381 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
4441 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
4501 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
4561 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
4621 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
4681 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
4741 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
4801 atcagagcag attgtactga gagtgcacca tatatgcggt gtgaaatacc gcacagatgc
4861 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
4921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
4981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
5041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
5101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
5161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
5221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
5281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
5341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
5401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
5461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
5521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
5581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
5641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
5701 aacgaaaact cacgttaagg gattttggtc atgacctatt tgtttatttt tctaaataca
5761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat catgattgaa
5821 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5881 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5941 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
6001 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
6061 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
6121 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
6181 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
6241 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
6301 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
6361 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
6421 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
6481 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
6541 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
6601 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
6661 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
6721 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6781 ataatctcat gccatcgtgc cgccagaaat tgtaaacgtt aatattttgt taaaattcgc
6841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
6901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
6961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
7021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
7081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
7141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
7201 agcggtcacg ctgcgcgtaa ccaccacacc
//
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pET-43.1c(+)质粒 pET43.1c载体应用举例
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