| 出品公司: | Takara |
|---|---|
| 载体名称: | pAUR123 |
| 质粒类型: | 酵母表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | ADH1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6982bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | 3602 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pAUR123载体质粒基本信息
pAUR123质粒图谱载体图谱和pAUR123载体序列质粒序列多克隆位点信息
pAUR123质粒载体简介
pAUR vectors have a mutant AUR1-C gene derived from S. cerevisiae as
a selective marker for yeast transformation system. pAUR123 is a protein
expression vector and E. coli -yeast shuttle vector derived from pAUR112.
It contains a constitutive promoter of ADH1 gene3 (Alcohol dehydrogenase
1 gene) for protein expression in yeast cells.
(Note: This vector is not suitable for high expression of protein.)
pAUR123质粒序列载体序列
LOCUS AB012284 6982 bp DNA linear SYN 18-AUG-2007
DEFINITION Shuttle vector pAUR123 gene for Aur1-C, complete cds.
ACCESSION AB012284
VERSION AB012284.1 GI:2992151
KEYWORDS Aur1-C variant; aureobasidin resistant protein.
SOURCE Shuttle vector pAUR123
ORGANISM Shuttle vector pAUR123
other sequences; artificial sequences; vectors.
REFERENCE 1
AUTHORS Hashida-Okado,T.
TITLE Yeast-E. coli shuttle vector pAUR123
JOURNAL Published Only in Database (1998)
REFERENCE 2 (bases 1 to 6982)
AUTHORS Hashida-Okado,T.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-1998) Takashi Hashida-Okado, Takara Shuzo
Co.,Ltd., Biotechnology Research Laboratories; Seta 3-4-1, Otsu,
Shiga 520-2193, Japan (E-mail:okadot@takara.co.jp,
Tel:81-77-543-7298, Fax:81-77-543-2494)
FEATURES Location/Qualifiers
source 1..6982
/organism="Shuttle vector pAUR123"
/mol_type="other DNA"
/db_xref="taxon:75307"
gene 4017..5222
/gene="AUR1-C"
CDS 4017..5222
/gene="AUR1-C"
/function="aureobasidin resistant"
/note="variant"
/codon_start=1
/transl_table=11
/product="Aur1-C"
/protein_id="BAA25321.1"
/db_xref="GI:2992152"
/translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSD
WVHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALY
FTSSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGA
PFVVAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDM
HGSPGGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPL
FIAYVCWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSI
EKYDISKSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATS
ITSLGVKRA"
ORIGIN
1 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
61 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
121 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
481 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
781 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
1021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
1081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
1141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
1201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
2041 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
2101 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
2161 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
2221 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
2281 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
2341 cagctgggcc attctcatga agaatatctt gaatttattg tcatattact agttggtgtg
2401 gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc
2461 ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac
2521 tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag
2581 tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc
2641 tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct
2701 taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt
2761 taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca
2821 tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat
2881 tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta
2941 ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata
3001 aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg
3061 gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca
3121 gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta
3181 tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc
3241 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa
3301 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat
3361 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc
3421 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag
3481 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac
3541 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat
3601 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctc gaatttcgag
3661 aaagtgccca tcagtgttca tatttgttac aaagtggttt tctgatttgg tactactgca
3721 gaggcgtatt ttttgcttca gttaccatag cgtaagaaca ctagcgactt ttgttcgtga
3781 accaacagag taggatttct actgctacat ctcttaggta gttggttagt ccgatcgctc
3841 acttttggtt gttgttaagt acttcataag tttatccttt tcctttttca cactgagcta
3901 ctttgggtat agcttttggc ccaaggatct ttgaattttc tccaaaagta ctttatttta
3961 tatcctacag gttgcggttt tcatatttta aaaagctttt aatcattcct ttgcgtatgg
4021 caaacccttt ttcgagatgg tttctatcag agagacctcc aaactgccat gtagccgatt
4081 tagaaacaag tttagatccc catcaaacgt tgttgaaggt gcaaaaatac aaacccgctt
4141 taagcgactg ggtgcattac atcttcttgg gatccatcat gctgtttgtg ttcattacta
4201 atcccgcacc ttggatcttc aagatccttt tttattgttt cttgggcact ttattcatca
4261 ttccagctac gtcacagttt ttcttcaatg ccttgcccat cctaacatgg gtggcgctgt
4321 atttcacttc atcgtacttt ccagatgacc gcaggcctcc tattactgtc aaagtgttac
4381 cagcggtgga aacaatttta tacggcgaca atttaagtga tattcttgca acatcgacga
4441 attccttttt ggacatttta gcatggttac cgtacggact atttcattat ggggccccat
4501 ttgtcgttgc tgccatctta ttcgtatttg gtccaccaac tgttttgcaa ggttatgctt
4561 ttgcatttgg ttatatgaac ctgtttggtg ttatcatgca aaatgtcttt ccagccgctc
4621 ccccatggta taaaattctc tatggattgc aatcagccaa ctatgatatg catggctcgc
4681 ctggtggatt agctagaatt gataagctac tcggtattaa tatgtatact acatgttttt
4741 caaattcctc cgtcattttc ggtgcttttc cttcactgca ttccgggtgt gctactatgg
4801 aagccctgtt tttctgttat tgttttccaa aattgaagcc cttgtttatt gcttatgttt
4861 gctggttatg gtggtcaact atgtatctga cacaccatta ttttgtagac cttatggcag
4921 gttctgtgct gtcatacgtt attttccagt acacaaagta cacacattta ccaattgtag
4981 atacatctct tttttgcaga tggtcataca cttcaattga gaaatacgat atatcaaaga
5041 gtgatccatt ggctgcagat tcaaacgata tcgaaagtgt ccctttgtcc aacttggaac
5101 ttgactttga tcttaatatg actgatgaac ccagtgtaag cccttcgtta tttgatggat
5161 ctacttctgt ttctcgttcg tccgccacgt ctataacgtc actaggtgta aagagggctt
5221 aatgagtatt ttatctgcaa ttacggatac ggttggtctt atgtagatac atataaatat
5281 atatcttttt ctttcttttt cttagtcagg attgtcgttt agcataatat acatgtagtt
5341 tatttaatca cataccactg attatcttta gaattttata aatttttgaa ataaatgggt
5401 ggcttttaat ggtgtctatg ttaagtgagg cttttagaat gctcttcctg ctttgtttat
5461 tatatgtgta tgaaagatat gtatgtattt acatgtgttt gtagcgtccc cagtcaaaac
5521 ctgtgcgcta tacctaaatg gattgataat cttcattcac taattctaaa atagacttct
5581 tccccaaaga acggtgtaac gatgaggctc tatccagctg cttatctaaa tcaactttaa
5641 cgatggatga tcttatgaca cggggatctt tctttaaagt tcttagaatt tcagactgta
5701 ccgcagctga tgaatcaaac agcattaaaa agtgatatgc tcgaaaatgt ttttcctggt
5761 ctttcttcat tattttagga agatacctta tgcccatggg tacaatgtcc ctcaccacac
5821 ctctgttttg aataatcagt ttcccgattg tggaagacaa ttcttttgct tccaactttg
5881 gcgcattgga gttggttatg cgaacaagtc cgatcagctc ataaagcatc ttagtgaaaa
5941 gggtggtttt gcgttattct ttcctctgga tcctctagct ccctaacatg taggtggcgg
6001 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca
6061 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt
6121 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta
6181 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc
6241 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca
6301 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg
6361 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct
6421 tccttccagt tacttgaatt tgaaataaaa aaagtttgct gtcttgctat caagtataaa
6481 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
6541 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact ggtacccggg
6601 tcgactcgag ctctagaggt taactaagcg aatttcttat gatttatgat ttttattatt
6661 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa
6721 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag
6781 catgaggtcg ctcttattga ccacatctct accggcatgc cgagcaaatg cctgcaaatc
6841 gctccccatt tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg
6901 tgtgtatttt atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcca
6961 cagcctagcc ttcagtcgag gc
//
pAUR123载体图谱质粒图谱pdf版和pAUR123载体序列质粒序列等相关资料下载
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