| 出品人: | Irene Castano Navarro（2015） |
|---|---|
| 载体名称: | pYC119 |
| 质粒类型: | 酿酒酵母载体；复制型载体；过表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TEF1 |
| 载体大小: | 5796 bp |
| 5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
| 3' 测序引物及序列: | KS primer 5’ CGAGgtcgacggtatcg 3’ |
| 载体标签: | 3XFLAG tag (C-ter) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3 |
| 克隆菌株: | DH10B感受态细胞 ,30°C |
| 宿主细胞（系）: | 出芽酵母（酿酒酵母）、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #63916 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYC119载体质粒基本信息
pYC119质粒图谱载体图谱和pYC119载体序列质粒序列多克隆位点信息
pYC119质粒载体简介
pYC119质粒序列载体序列
LOCUS pYC119 5796 bp DNA SYN
DEFINITION pYC119
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5796
/organism="pYC119"
/mol_type="other DNA"
promoter 668..696
/label="AmpR_promoter"
gene 738..1598
/label="Ampicillin"
/gene="Ampicillin"
CDS 738..1598
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 1753..2372
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2724..2746
/label="M13_pUC_rev_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2745..2763
/label="M13_reverse_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2780..2799
/label="T3_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3218..3234
/label="pBluescriptSK_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3253..3264
/label="EK"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3313..3327
/label="EK"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(3372..3388)
/label="pBluescriptKS_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 3624..4208
/label="ORF frame 3"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter complement(3802..3820)
/label="T7_promoter"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter complement(3830..3846)
/label="M13_forward20_primer"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature complement(3839..3861)
/label="M13_pUC_fwd_primer"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
CDS complement(4577..5380)
/label="ORF frame 3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
gene complement(4580..5380)
/label="URA3"
/gene="URA3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS 4869..5345
/label="ORF frame 3"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaGCAGGT TAGACTAACA TGCAAAAAGT TTATATTCAT TTTTGTAGTT ATTCTTGTTT
121 TCATTAGGAT GATTACACAC TGTGGATGTG GATTAATCGA TCATTGTTAA TTTAGTGTAG
181 CTACTACCAT TTCAAACAAA ACGTAAATTC TTGCAGTCGT ACTGGATCTG TGAATCTATT
241 AGTATATATG AATTAAAGTA GCTTGTACAT ATTATTCTGT TGAATCATAT CGCAGAGCAT
301 TGGTTGAAAT CCAAAATATA AAAATGTAAT ATCACAAAAA ATAATACTAA TTCTAACATT
361 AATGGGTCAG ATTTTTAGTG AATACTTAAA TTTATAATAT CGTCTATTTA AGCTAGCAAA
421 TGGAACAACA TTTAAAGTAA GAACATCATA TCTACATGAA AATGTATATT TCAATCTGAC
481 TAATAACGCA GAGCACATCT TTCAGTGATG TCTGTCACAT GATCAAAAAG AATTGTATTT
541 AAATAATTCA TAATAAAAGC TTAAAAAATT Acaataatga aaataaagtA AATAATGACA
601 TGGGTAAGAG TTCCGAAATA GAATCTTAGT GTACAAAACA AAAATTGCAT CATTAGAGAT
661 CCCCacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
721 attgaaaaag gaagagtATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
781 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
841 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
901 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
961 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
1021 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
1081 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
1141 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
1201 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
1261 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
1321 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
1381 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
1441 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
1501 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
1561 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAAct gtcagaccaa gtttactcat
1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gAGCTCATAG
2821 CTTCAAAATG TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC
2881 CGTACCACTT CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG
2941 GTGTCGTTAA TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT
3001 TTTCTTCGTC GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT
3061 TTTTTGATTT TTTTCTCTTT CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT
3121 CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC
3181 TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGTTTTCT AGaactagtg GATCCTAGGA
3241 GCAGACTACA AAGATGACGA TGACAAAGGA GCCGATTATA AGGACGATGA CGATAAAGGG
3301 GCTGACTACA AAGATGACGA TGACAAGTGA TAAgatcccc cgggctgcag gaattcgata
3361 tcaagcttat cgataccgtc gacCTCGAGA ACACAACCCA CAGCTACCAC CATCAACAAT
3421 ATTTATATAT ATAACGTACA CATAGAAATC ACACAAACAG AGTATTTATT CTTAACTACA
3481 TGAACTACCA TCAGACCGTC TGGGCCACTA TATAATGTGC CATTCATAAA CGTGATCACT
3541 TTACGTAGCA GGCAACCCCA GGTGAAAATT TTTCAGCGAG CTGCCAGATT GTCAGGTGAA
3601 AAACTGAAAA AAACTTCTGG GCGATGAGCT TGTGGCGGGA AATTAAGTAT ATAAAGCAGT
3661 TAGTTTCTTC TGCTTCTTGT GGTTCTGGGT ATTCTTGTAA TATTTGGAAG AATAGAAAAT
3721 CAAGACTACT GCCTTTCTTT TCATATTATA GAGGAAGAAA TACGCACGAA CACGATATAG
3781 AGGTAAAGGT ACCcaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
3841 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
3901 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
3961 agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg
4021 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
4081 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4141 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4201 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4261 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4321 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
4381 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
4441 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg
4501 gtaataactg atataattaa attgaagctc taatttgtga gtttagtata catgcattta
4561 cttataatac agttttTTAG TTTTGCTGGC CGCATCTTCT CAAATATGCT TCCCAGCCTG
4621 CTTTTCTGTA ACGTTCACCC TCTACCTTAG CATCCCTTCC CTTTGCAAAT AGTCCTCTTC
4681 CAACAATAAT AATGTCAGAT CCTGTAGAGA CCACATCATC CACGGTTCTA TACTGTTGAC
4741 CCAATGCGTC TCCCTTGTCA TCTAAACCCA CACCGGGTGT CATAATCAAC CAATCGTAAC
4801 CTTCATCTCT TCCACCCATG TCTCTTTGAG CAATAAAGCC GATAACAAAA TCTTTGTCGC
4861 TCTTCGCAAT GTCAACAGTA CCCTTAGTAT ATTCTCCAGT AGATAGGGAG CCCTTGCATG
4921 ACAATTCTGC TAACATCAAA AGGCCTCTAG GTTCCTTTGT TACTTCTTCT GCCGCCTGCT
4981 TCAAACCGCT AACAATACCT GGGCCCACCA CACCGTGTGC ATTCGTAATG TCTGCCCATT
5041 CTGCTATTCT GTATACACCC GCAGAGTACT GCAATTTGAC TGTATTACCA ATGTCAGCAA
5101 ATTTTCTGTC TTCGAAGAGT AAAAAATTGT ACTTGGCGGA TAATGCCTTT AGCGGCTTAA
5161 CTGTGCCCTC CATGGAAAAA TCAGTCAAGA TATCCACATG TGTTTTTAGT AAACAAATTT
5221 TGGGACCTAA TGCTTCAACT AACTCCAGTA ATTCCTTGGT GGTACGAACA TCCAATGAAG
5281 CACACAAGTT TGTTTGCTTT TCGTGCATGA TATTAAATAG CTTGGCAGCA ACAGGACTAG
5341 GATGAGTAGC AGCACGTTCC TTATATGTAG CTTTCGACAT gatttatctt cgtttcctgc
5401 aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt tcttcaacac
5461 tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct tccttctgtt
5521 cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa gaataaaaaa
5581 aaaatgatga attgaattga aaagctgtgg tatggtgcac tctcagtaca atctgctctg
5641 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
5701 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agGtgcatgt
5761 gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
