| 出品公司: | -- |
|---|---|
| 载体名称: | pLVTHM |
| 质粒类型: | 哺乳动物细胞慢病毒表达载体, RNAi载体， Cre/loxP载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | EF-1 alpha |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 11085 bp |
| 5' 测序引物及序列: | EF-1a Forward: TCAAGCCTCAGACAGTGGTTC |
| 3' 测序引物及序列: | EGFP-N: CGTCGCCGTCCAGCTCGACCAG |
| 载体标签: | C-EGFP |
| 载体抗性: | 氨苄 |
| 备注: | 载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。pLVTHM载体用来替代pLVTH，允许shRNA定向 克隆入载体。 |
| 产品目录号: | -- |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVTHM载体质粒基本信息
pLVTHM质粒图谱载体图谱和pLVTHM载体序列质粒序列多克隆位点信息
pLVTHM质粒载体简介
pLVTHM allows for direct cloning of shRNAs into the lentiviral vectors and replaces the old pLVTH system from Trono lab.
pLVTHM is similar to pLVTH, but contains a 3bp substitution that generates a unique MluI site for direct cloning of an shRNA into MluI-ClaI.
Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation (this site does not appear in the depositor's full sequence). The plasmid needs to be grown in a Dam+ bacterial strain in order to use ClaI for cloning.
Note that ClaI has lower salt concentration requirements than MluI. One way to handle this is to digest with ClaI for 45 minutes, followed by addition of diluted MluI buffer and MluI, then incubation for 90 more minutes. Be sure not to use a large amount of vector (1.7 ug to 2.5 ug is known to work).
Also, if your shRNA is already in pSUPER (or another plasmid under the control of the PolIII promoter) you may use the EcoRI-ClaI sites to replace the H1 promoter in pLVTHM with the H1-shRNA cassette from pSUPER (or another plasmid).
pLVTHM质粒序列载体序列
LOCUS pLVTHM 11085 bp DNA circular SYN
DEFINITION pLVTHM
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..11085
/organism="pLVTHM"
/mol_type="other DNA"
misc_feature 455..635
/label="truncHIV-1_3_LTR"
misc_feature 455..635
/label="HIV-1_5_LTR"
misc_feature 746..790
/label="HIV-1_psi_pack"
CDS 1178..2056
/label="ORF frame 2"
misc_feature 1300..1533
/label="RRE"
CDS complement(1906..3039)
/label="ORF frame 1"
promoter 2129..3299
/label="EF1a_promoter"
misc_feature 3247..3267
/label="EF1a_fwd_primer"
misc_feature 3351..3366
/label="cPPT"
CDS 3554..4291
/label="ORF frame 2"
gene 3557..4270
/label="EGFP"
/gene="EGFP"
misc_feature complement(3599..3620)
/label="EGFP_N_primer"
misc_feature 3737..3766
/label="Y66 (EGFP)"
misc_feature 4207..4228
/label="EGFP_C_primer"
misc_feature 4345..4932
/label="WPRE"
CDS complement(4430..4933)
/label="ORF frame 3"
CDS 4433..4993
/label="ORF frame 2"
misc_feature 5003..5018
/label="cPPT"
misc_feature 5003..5024
/label="U3PPT"
misc_feature 5085..5372
/label="TRE"
misc_feature 5533..5552
/label="H1_primer"
misc_feature 5647..5680
/label="loxP"
misc_feature 5700..5880
/label="HIV-1_5_LTR"
misc_feature 5700..5880
/label="truncHIV-1_3_LTR"
misc_feature complement(5906..5923)
/label="Sp6_primer"
gene 6161..6424
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(6213..6232)
/label="pBRrevBam_primer"
misc_feature 6565..6587
/label="pGEX_3_primer"
promoter 6746..6774
/label="AmpR_promoter"
gene 6816..7676
/label="Ampicillin"
/gene="Ampicillin"
CDS 6816..7676
/label="ORF frame 3"
rep_origin 7831..8450
/label="pBR322_origin"
misc_feature complement(8676..8696)
/label="pBABE_3_primer"
misc_feature complement(8682..9135)
/label="SV40_enhancer"
promoter 8694..8962
/label="SV40_promoter"
rep_origin 8861..8938
/label="SV40_origin"
misc_feature 8923..8942
/label="SV40pro_F_primer"
misc_feature 10143..10158
/label="SV40_int"
misc_feature 10164..10211
/label="SV40_3_splice"
CDS 10288..10761
/label="ORF frame 1"
misc_feature 10339..10359
/label="SV40 NLS"
misc_feature 10345..10359
/label="NLS"
terminator 10787..10918
/label="SV40_PA_terminator"
misc_feature 10875..10894
/label="EBV_rev_primer"
ORIGIN
1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct
2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc
2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag
3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag
3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct
3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa
4321 cttcggggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac
4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
4741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
4921 cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact tacaaggcag
4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
5041 aacgaagaca agatgggatc aattcaccat gctagtggat ccccctcgag tttaccactc
5101 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
5161 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
5221 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
5281 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
5341 agtttaccac tccctatcag tgatagagaa aagtgaaagt cggggctgca ggaattcgaa
5401 cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac
5461 ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc
5521 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat
5581 ttgggaatct tataagttct gtatgagacc acgcgtctcc aagcttatcg ataccgtcgc
5641 atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg
5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
5881 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
5941 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
6001 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
6061 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
6121 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
6181 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
6241 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
6301 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
6361 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
6421 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
6481 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
6541 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
6601 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
6661 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
6721 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
6781 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
6841 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
6901 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
6961 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
7021 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
7081 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
7141 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
7201 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
7261 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
7321 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
7381 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
7441 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
7501 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
7561 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
7621 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
7681 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
7741 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
7801 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
7861 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
7921 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
7981 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
8041 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
8101 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
8161 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
8221 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
8281 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
8341 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
8401 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
8461 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
8521 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
8581 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
8641 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
8701 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
8761 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
8821 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
8881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
8941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
9001 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
9061 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
9121 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
9181 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
9241 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
9301 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
9361 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
9421 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
9481 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
9541 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
9601 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
9661 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
9721 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
9781 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
9841 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
9901 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
9961 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
10021 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
10081 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
10141 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
10201 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
10261 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
10321 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
10381 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
10441 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
10501 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
10561 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
10621 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
10681 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
10741 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
10801 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
10861 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
10921 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
10981 tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
11041 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtag
//
