LOCUS pLVX-TRE3G-Luc Control 9452 bp DNA circular 31-MAY-2011
COMMENT Created by Clontech Laboratories Inc.
COMMENT 631191,631193,631187, 631189, 631191, 631193, 631360, 631361,631362,631187,631189,631349,631350,631351,631352,631353,631354,631355,631356,631357, 635087
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1..635
/label=5'\LTR
misc_feature 685..822
/label=Packaging\signal
misc_feature 1303..1536
/label=RRE
misc_feature 2028..2151
/label=cPPT
misc_feature 4233..4736
/label=pPGK
misc_feature 4757..5356
/label=Puro(R)
misc_feature 5374..5965
/label=WPRE
3'clip 6169..6805
/label=3'\LTR
misc_feature complement(7275..7945)
/label=pUC\ori
misc_feature 2566..4217
/label=Luciferase
misc_feature 2210..2555
/label=pTRE3G
misc_feature complement(8090..8950)
/label=Amp(R)
BASE COUNT 2452 a 2295 c 2424 g 2281 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
2521 ctggagcaat tccacaacac ttttgtctta tacttggatc caccatggaa gacgccaaaa
2581 acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct ggagagcaac
2641 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
2701 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
2761 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
2821 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
2881 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
2941 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
3001 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
3061 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
3121 ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact gggttaccta
3181 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
3241 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
3301 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
3361 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
3421 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
3481 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
3541 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
3601 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
3661 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
3721 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
3781 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
3841 gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
3901 gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc aacatcttcg
3961 acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
4021 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
4081 taacaaccgc caaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
4141 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
4201 gaaagtccaa attgtaaaat acgcgtgaat tctaccgggt aggggaggcg cttttcccaa
4261 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
4321 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
4381 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
4441 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
4501 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
4561 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
4621 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
4681 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
4741 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
5341 caagcccggt gcctgacggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt
5401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
5461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
5521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
5581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
5641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
5701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
5761 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
5821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
5881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
5941 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
6001 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
6061 aagaggagga ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact
6121 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
6181 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
6241 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
6301 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
6361 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
6421 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
6481 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
6541 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
6601 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
6661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
6721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
6781 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
6841 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
6901 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
6961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
7021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
7081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
7141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
7201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
7261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
7321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
7381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
7441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
7501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
7561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
7621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
7681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
7741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
7801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
7861 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
9061 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
9241 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
9301 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
9361 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt
9421 atttgttaaa tatgtactac aaacttagta gt
//