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pLPCX载体质粒基本信息
pLPCX质粒图谱载体图谱和pLPCX载体序列质粒序列多克隆位点信息
pLPCX质粒载体简介
pLPCX质粒序列载体序列
LOCUS pLPCX 6264 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Retroviral vector for transient or stable constitutive expression of
a gene.
ACCESSION .
VERSION .
KEYWORDS pLPCX
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6264)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Wednesday, February 19, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6264
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
LTR 1..589
/note="5' LTR"
/note="long terminal repeat from Moloney murine sarcoma
virus"
misc_feature 652..851
/note="MMLV Psi"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1052..1468
/note="gag (truncated)"
/note="truncated MMLV gag gene lacking the start codon"
CDS 1565..2164
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
enhancer 2339..2642
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 2643..2846
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 2887..2963
/label=MCS
/note="MCS"
/note="multiple cloning site"
LTR 3003..3596
/note="3' LTR"
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind complement(3849..3882)
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(3942..3975)
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(4266..4854)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5025..5885)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5886..5990)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
2881 ggactcagat ctcgagctca agcttcgaat tcgttaggcc attaaggcct gtcgacaagc
2941 ggccgcctcg gccaaacatc gataaaataa aagattttat ttagtctcca gaaaaagggg
3001 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
3061 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
3121 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
3181 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
3241 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
3301 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
3361 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
3421 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
3481 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
3541 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg
3601 ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa
3661 gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
3721 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
3781 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
3841 gtgtactgat aacttcgtat aatgtatgct atacgaagtt attaggtctg aagaggagtt
3901 tacgtccagc caagctaggg ccgcgatccg gaacccttaa tataacttcg tataatgtat
3961 gctatacgaa gttatcagta ctggcttaac tatgcggcat cagagcagat tgtactgaga
4021 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
4081 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
4141 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
4201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
4261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
4321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
4381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
4441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
4501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
4561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
4621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
4681 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
4741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
4801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
4861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
5041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
5101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
5161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
5221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
5281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
5341 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
5401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
5461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
5521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
5581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
5641 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
5701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
5761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
5821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
5881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
6001 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
6061 aggcgtatca cgaggccctt tcgtcttcaa gaattgctag caattgctag caattgctag
6121 caattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
6181 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
6241 ccgcggccct tccgtttctt tgct
//
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