| 出品公司: | Clontech |
|---|---|
| 载体名称: | pMSCVhyg, pMSCV-hygro, pMSCV hyg |
| 质粒类型: | 哺乳动物细胞逆病毒基因表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | LTR |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6945bp |
| 5' 测序引物及序列: | MSCV-F: CCCTTGAACCTCCTCGTTCGACC |
| 3' 测序引物及序列: | pMSCV-R: GAGACGTGCTACTTCCATTTGTC |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | 潮霉素（Hygromycin） |
| 备注: | 逆病毒表达载体，优化后，有利于在造血干细胞中和癌细胞中进行基因表达。 |
| 产品目录号: | 634401 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
pMSCVhyg载体 pMSCV-Hygro质粒基本信息
pMSCVhyg质粒图谱 pMSCV-Hygro载体图谱和pMSCVhyg载体序列 pMSCV-Hygro质粒序列多克隆位点信息
pMSCVhyg质粒 pMSCV-Hygro载体简介
pMSCVhyg质粒序列 pMSCV-Hygro载体序列
LOCUS pMSCVhyg 6945 bp DNA circular 4-DEC-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 631461,634401
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 3368..3851
/label=3'\PCMV\LTR
LTR 1..515
/label=5'\MMSV\LTR
CDS complement(5154..6011)
/label=Amp(R)
rep_origin 4391..4391
/label=pUC\origin
CDS 1955..2980
/label=Hyg(R)
promoter 1429..1937
/label=Phosphoglycerate\Kinase\Promoter
misc_signal 516..1404
/label=Extended\packaging\signal
BASE COUNT 1578 a 1900 c 1857 g 1610 t
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg
1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
1921 catctccggg cctttcgacc tgcatcccgc caccatgaaa aagcctgaac tcaccgcgac
1981 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
2041 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
2101 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
2161 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
2221 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
2281 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
2341 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
2401 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
2461 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
2521 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
2581 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
2641 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
2701 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
2761 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
2821 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
2881 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
2941 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa
3001 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga
3061 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc
3121 tgggtcgcgg gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag
3181 ggcacgattg tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg
3241 ccgcccgtgc ctgccgattg ggtgcagatc cgtcgacctg cagccaagct tatcgataaa
3301 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
3361 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata
3421 gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat
3481 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
3541 cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
3601 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
3661 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc
3721 cgatagactg cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
3781 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
3841 ggggtctttc atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt
3901 cgaatattaa gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact
3961 cctgaaatag ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat
4021 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
4081 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
4141 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
4201 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
4261 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4321 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
4381 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
4441 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
4501 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
4561 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
4621 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
4681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
4741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4801 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
4861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4921 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
4981 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
5041 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
5101 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
5161 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
5221 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
5281 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
5341 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
5401 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
5461 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
5521 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
5581 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
5641 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
5701 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
5761 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
5821 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
5881 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
5941 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
6001 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
6061 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
6121 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
6181 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
6241 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
6301 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
6361 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
6421 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
6481 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
6541 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
6601 gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
6661 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
6721 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
6781 ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg
6841 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
6901 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaa
//
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