| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | SuperTopFlash |
| 质粒类型: | 信号通路报告系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4997bp |
| 5' 测序引物及序列: | RVPrimer3: CTAGCAAAATAGGCTGTCCC |
| 3' 测序引物及序列: |
LucNRev: CCTTATGCAGTTGCTCTCC
|
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | 克隆菌株DH5alpha |
| 产品目录号: | 12456 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
SuperTopFlash载体质粒基本信息
SuperTopFlash质粒图谱载体图谱和SuperTopFlash载体序列质粒序列多克隆位点信息
SuperTopFlash质粒载体简介
SuperTopFlash质粒序列载体序列
LOCUS M50 Super 8x TOPFlash 4997 bp DNA SYN
DEFINITION M50 Super 8x TOPFlash
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4997
/organism="M50 Super 8x TOPFlash"
/mol_type="other DNA"
gene 267..1919
/label="firefly_Luciferase"
/gene="firefly_Luciferase"
CDS 267..1919
/label="ORF frame 3"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
misc_feature complement(333..351)
/label="LucNrev_primer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
misc_feature complement(1971..1990)
/label="EBV_rev_primer"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
gene complement(3259..4119)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
CDS complement(3259..4119)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(4161..4189)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4268..4574
/label="f1_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS complement(4784..404)
/label="ORF frame 1"
/translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
misc_feature 4837..4990
/label="TB"
/translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
terminator 4837..4885
/label="synth_PA_terminator"
/translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
terminator 4837..4990
/label="up_PA_terminator"
/translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
misc_feature 4939..4958
/label="RVprimer3_primer"
/translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
ORIGIN
1 ggtaccgagc tcttacgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg
61 ggtaagatca aaggcgcgag atcaaagggg gtaagatcaa agggggtaag atcaaagggg
121 gtaagatcaa aggggcgcgc ccgcgtgcta gcccgggctc gagatctaga ctctagaggg
181 tatataatgg aagctcgaat tccagcttgg cattccggta ctgttggtaa aaagcttggc
241 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
301 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
361 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
421 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
481 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
541 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
601 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
661 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
721 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
781 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
841 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
901 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
961 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
1021 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
1081 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
1141 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
1201 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
1261 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
1321 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
1381 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
1441 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
1501 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
1561 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
1621 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
1681 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
1741 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
1801 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
1861 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
1921 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
1981 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
2041 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
2101 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
2161 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
2221 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
2281 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
2461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
2521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
2581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
2641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
2701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
2761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
2821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
2881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
2941 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
3001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
3061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
3121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
3181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
3241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
3301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
3361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
3421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
3481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
3541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
3601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
3661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
3721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
3781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
3841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
3901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
3961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
4021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
4081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
4141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
4201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
4261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
4501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
4621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4681 taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga
4741 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
4801 taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta
4861 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
4921 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
4981 acatttctct atcgata
//
