Cloning vector allowing mobilization into a wide range of Gramand
Gram+ bacteria. After
mobilization, the plasmid can be maintained by integration into the host chromosome via
homologous recombination. Excision of the intervening plasmid sequence by a double crossover
event can be facilitated by selection on medium containing 10% sucrose.The sacB gene has
been modified to eliminate the HindIII and EcoRI sites in the coding region.
Designation: pK18mobsacB
LOCUS pK18mobsacB 5721 bp DNA 17-AUG-2006
DEFINITION Cloning vector pK18mobsacB.
ACCESSION
VERSION
KEYWORDS .
SOURCE Cloning vector pK18mobsacB.
ORGANISM Cloning vector pK18mobsacB
Unclassified.
REFERENCE 1 (bases 1 to 5721)
AUTHORS Schafer,A., Tauch,A., Jager,W., Kalinowski,K., Thierbach,G. and
Puhler,A.
TITLE Small mobilizable multi-purpose cloning vectors derived from the
Escherichia coli plasmids pK18 and pK19: selection of defined
deletions in the chromosome of Corynebacterium glutamicum
JOURNAL Gene 145, 69-73 (1994)
REFERENCE 2 (bases 1 to 5721)
AUTHORS Ishikawa,J.
TITLE Unofficial nucleotide sequence of pK18mobsacB
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 5721)
AUTHORS Ishikawa,J.
TITLE Direct Submission
JOURNAL Submitted (17-AUG-2006) Bioactive Molecules, NIID
FEATURES Location/Qualifiers
source 1..5721
/organism="Cloning vector pK18mobsacB"
/mol_type="Other DNA"
CDS complement(185..469)
/gene="lacZ"
/codon_start=1
/product="beta-galactosidase"
oriT complement(2209..2736)
/note="RP4 origin of transfer"
CDS complement(2886..4307)
/gene="sacB"
/codon_start=1
/product="levansucrase"
CDS complement(4799..5593)
/gene="neo"
/note="confers resistances to neomycin and kanamycin"
/codon_start=1
/product="aminoglycoside 3'-O-phosphotransferase"
BASE COUNT 1343 a 1426 c 1414 g 1538 t
ORIGIN
1 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc
61 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
121 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct
181 tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagctagc ttatcgcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
1561 cttttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccc
1621 tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta aatcagtaag
1681 ttggcagcat cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc
1741 gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg
1801 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
1861 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctgatag aaacagaagc
1921 cactggagca cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg
1981 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
2041 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
2101 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
2161 tttttgagtt atcgagattt tcaggagctc tttggcatcg tctctcgcct gtcccctcag
2221 ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga tattccacaa aacagcaggg
2281 aagcagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
2341 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
2401 tggtgtatcc aacggcgtca gcggggcagg ataggtgaag taggcccacc cgcgagcggg
2461 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
2521 cgaggctggc cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc
2581 aagccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga
2641 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc
2701 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gggcgtcccg
2761 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
2821 tggcaggttg ggcgtcgctt ggtcggtcat ttcgctcggt accatcggca ttttcttttg
2881 cgtttttatt tgttaactgt taattgtcct tgttcaagga tgctgtcttt gacaacagat
2941 gttttcttgc ctttgatgtt cagcaggaag ctcggcgcaa acgttgattg tttgtctgcg
3001 tagaatcctc tgtttgtcat atagcttgta atcacgacat tgtttccttt cgcttgaggt
3061 acagcgaagt gtgagtaagt aaaggttaca tcgttaggat caagatccat ttttaacaca
3121 aggccagttt tgttcagcgg cttgtatggg ccagttaaag aattagaaac ataaccaagc
3181 atgtaaatat cgttagacgt aatgccgtca atcgtcattt ttgatccgcg ggagtcagtg
3241 aacaggtacc atttgccgtt cattttaaag acgttcgcgc gttcaatttc atctgttact
3301 gtgttagatg caatcagcgg tttcatcact tttttcagtg tgtaatcatc gtttagctca
3361 atcataccga gagcgccgtt tgctaactca gccgtgcgtt ttttatcgct ttgcagaagt
3421 ttttgacttt cttgacggaa gaatgatgtg cttttgccat agtatgcttt gttaaataaa
3481 gattcttcgc cttggtagcc atcttcagtt ccagtgtttg cttcaaatac taagtatttg
3541 tggcctttat cttctacgta gtgaggatct ctcagcgtat ggttgtcgcc tgagctgtag
3601 ttgccttcat cgatgaactg ctgtacattt tgatacgttt ttccgtcacc gtcaaagatt
3661 gatttataat cctctacacc gttgatgttc aaagagctgt ctgatgctga tacgttaact
3721 tgtgcagttg tcagtgtttg tttgccgtaa tgtttaccgg agaaatcagt gtagaataaa
3781 cggatttttc cgtcagatgt aaatgtggct gaacctgacc attcttgtgt ttggtctttt
3841 aggatagaat catttgcatc gaatttgtcg ctgtctttaa agacgcggcc agcgtttttc
3901 cagctgtcaa tagaagtttc gccgactttt tgatagaaca tgtaaatcga tgtgtcatcc
3961 gcatttttag gatctccggc taatgcaaag acgatgtggt agccgtgata gtttgcgaca
4021 gtgccgtcag cgttttgtaa tggccagctg tcccaaacgt ccaggccttt tgcagaagag
4081 atatttttaa ttgtggacga atcaaattca gaaacttgat atttttcatt tttttgctgt
4141 tcagggattt gcagcatatc atggcgtgta atatgggaaa tgccgtatgt ttccttatat
4201 ggcttttggt tcgtttcttt cgcaaacgct tgagttgcgc ctcctgccag cagtgcggta
4261 gtaaaggtta atactgttgc ttgttttgca aactttttga tgttcatcgt tcatgtctcc
4321 ttttttatgt actgtgttag cggtctgctt cttccagccc tcctgtttga agatggcaag
4381 ttagttacgc acaataaaaa aagacctaaa atatgtaagg ggtgacgcca aagtatacac
4441 tttgcccttt acacatttta ggtcttgcct gctttatcag taacaaaccc gcgcgattta
4501 cttttcgacc tcattctatt agactctcgt ttggattgca actggtctat tttcctcttt
4561 tgtttgatag aaaatcataa aaggatttgc agactacggg cctaaagaac taaaaaatct
4621 atctgtttct tttcattctc tgtatttttt atagtttctg ttgcatgggc ataaagttgc
4681 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg
4741 gcaggtatat gtgatgggtt aaaaaggatc gatcctctag cgaaccccag agtcccgctc
4801 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
4861 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
4921 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
4981 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
5041 cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga
5101 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
5161 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
5221 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
5281 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
5341 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
5401 ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
5461 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
5521 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
5581 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
5641 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
5701 taccagaggg cgccccagct g
//
