| 出品公司: | -- |
|---|---|
| 载体名称: | pSH47 |
| 质粒类型: | 酵母基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | GAL |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6979 bp |
| 5' 测序引物及序列: | M13-F:TGTAAAACGACGGCCAGT |
| 3' 测序引物及序列: | M13-R:CAGGAAACAGCTATGAC |
| 载体筛选标记: | URA3 |
| 载体抗性: | 氨苄 |
| Genbank No. : | AF298782 |
| 备注: | Cre酶酵母表达质粒 |
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pSH47载体质粒基本信息
pSH47质粒图谱载体图谱和pSH47载体序列质粒序列多克隆位点信息
pSH47质粒载体简介
pSH47质粒转化到大肠杆菌中时抗性是氨苄。质粒包含有cre重组基因，该基因位于GAL启动子调控下。同时，pSH47载体质粒上面含有ura3酿酒酵母筛选标记。
pSH47载体质粒可以用于半乳糖诱导的cre酶表达。
pSH47质粒序列载体序列
LOCUS pSH47 6979 bp DNA circular SYN 11-MAR-2002
DEFINITION Recombinase expression vector pSH47, complete sequence.
ACCESSION AF298782
VERSION AF298782.1 GI:11344869
KEYWORDS .
SOURCE recombinase expression vector pSH47
ORGANISM recombinase expression vector pSH47
artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6979)
AUTHORS Guldener,U., Heck,S., Fielder,T., Beinhauer,J. and Hegemann,J.H.
TITLE A new efficient gene disruption cassette for repeated use in
budding yeast
JOURNAL Nucleic Acids Res. 24 (13), 2519-2524 (1996)
MEDLINE 96292254
PUBMED 8692690
REFERENCE 2 (bases 1 to 6979)
AUTHORS Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.
TITLE A second set of loxP marker cassettes for Cre-mediated multiple
gene knockouts in budding yeast
JOURNAL Nucleic Acids Res. 30 (6), E23 (2002)
MEDLINE 21882009
PUBMED 11884642
REFERENCE 3 (bases 1 to 6979)
AUTHORS Gueldener,U., Hegemann,J.H., Heck,S., Fiedler,T. and Beinhauer,J.D.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
40225, Germany
FEATURES Location/Qualifiers
source 1..6979
/organism="recombinase expression vector pSH47"
/mol_type="other DNA"
/db_xref="taxon:142964"
/note="derived from Saccharomyces cerevisiae"
gene 479..1282
/gene="URA3"
CDS 479..1282
/gene="URA3"
/codon_start=1
/transl_table=11
/product="orotidine-5'-phosphate decarboxylase"
/protein_id="AAG34514.1"
/db_xref="GI:11344870"
terminator 2066..2324
/note="from CYC1"
gene complement(2642..3673)
/gene="Cre"
CDS complement(2642..3673)
/gene="Cre"
/codon_start=1
/transl_table=11
/product="recombinase"
/protein_id="AAG34515.1"
/db_xref="GI:11344871"
promoter complement(3782..4249)
/note="from GAL1"
rep_origin 4692..4764
gene complement(5467..6327)
/gene="AmpR"
CDS complement(5467..6327)
/gene="AmpR"
/codon_start=1
/transl_table=11
/product="ampicillin resistance protein"
/protein_id="AAG34516.1"
/db_xref="GI:11344872"
BASE COUNT 1939 a 1562 c 1608 g 1869 t 1 others
ORIGIN
1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
61 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
241 gcaccatacc acagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt
301 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
361 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
421 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
481 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct
541 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac
601 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca
661 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc
721 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt
781 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc
841 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac
901 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac
961 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg
1021 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac
1081 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga
1141 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg
1201 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag
1261 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc
1321 acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt gaaataccgc
1381 acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa
1441 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
1501 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
1561 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
1621 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
1681 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
1741 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
1801 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
1861 gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
1921 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
1981 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
2041 atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt cgagcgtccc
2101 aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga
2161 aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac tataaaaaaa
2221 taaataggga cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg
2281 gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagg tcgacaattc
2341 caaccttacc caagagttcg ccaaactcag acatcacttt angcaaaacc gcgccgtgct
2401 tcttcctcgg tggcattcat cacgaaatgt tcagcactac gcatactttt gacaggaaac
2461 gcaacggata ttgagtcaat atcaggcatt ctatcgctca gctttacagt gacaatgacg
2521 gctggcgact gaatattagt gcttacagac agcactacat attttccgtc gatgttgaaa
2581 tcctttctca tatgtcacca taaatatcaa ataattatag caatcattta cgcgttaatg
2641 gctaatcgcc atcttccagc aggcgcacca ttgcccctgt ttcactatcc agggtacgga
2701 tatagttcat gacaatattt acattggtcc agccaccagc ttgcatgatc tccggtattg
2761 aaactccagc gcgggccata tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg
2821 ccaggtatct ctgaccagag tcatccttag cgccgtaaat caatcgatga gttgcttcaa
2881 aaatcccttc cagggcgcga gttgatagct ggctggtggc agatggcgcg gcaacaccat
2941 tttttctgac ccggcaaaac aggtagttat tcggatcatc agctacacca gagacggaaa
3001 tccatcgctc gaccagttta gttaccccca ggctaagtgc cttctctaca cctgcggtgc
3061 taaccagcgt tttcgttctg ccaatatgga ttaacattct cccaccgtca gtacgtgaga
3121 tatctttaac cctgatcctg gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc
3181 ccagaaatgc cagattacgt atatcctggc agcgatcgct attttccatg agtgaacgaa
3241 cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa
3301 cgttttcttt tcggatccgc cgcataacca gtgaaacagc attgctgtca cttggtcgtg
3361 gcagcccgga ccgacgatga agcatgttta gctggcccaa atgttgctgg atagttttta
3421 ctgccagacc gcgcgcctga agatatagaa gataatcgcg aacatcttca ggttctgcgg
3481 gaaaccattt ccggttattc aacttgcacc atgccgccca cgaccggcaa acggacagaa
3541 gcattttcca ggtatgctca gaaaacgcct ggcgatccct gaacatgtcc atcaggttct
3601 tgcgaacctc atcactcgtt gcatcgaccg gtaatgcagg caaattttgg tgtacggtca
3661 gtaaattgga catttaacac tcagataatg gttttaagta aagtgtacag gatcggctct
3721 gcccctcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccactag
3781 ttctagaatc cggggttttt tctccttgac gttaaagtat agaggtatat taacaatttt
3841 ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta
3901 ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat
3961 cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta
4021 tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt
4081 cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg
4141 cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt
4201 cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtactaga gctccagctt
4261 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
4321 tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa gcataaagtg
4381 taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc gctcactgcc
4441 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4501 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4561 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4621 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4681 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4741 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4801 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4861 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4921 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4981 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
5041 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5101 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
5161 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5221 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5281 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5341 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
5401 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5461 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5521 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5581 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5641 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5701 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5761 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5821 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5881 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5941 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6001 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6061 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6121 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6181 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6241 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6301 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6361 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6421 aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat cacgtgctat
6481 aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat agaaagtaaa
6541 aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac tctaggggga
6601 tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta atgccgaatt
6661 gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta cattttactt
6721 atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa tatatatgta
6781 aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct tcattccgta
6841 actcttctac cttctttatt tactttctaa aatccaaata caaaacataa aaataaataa
6901 acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg tgtaagttac
6961 aggcaagcga tccgtccta
//
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