| 出品公司: | -- |
|---|---|
| 载体名称: | pDC316-ZsGreen1-shRNA;pDC316-ZsGreen1-U6-shRNA |
| 质粒类型: | Admax腺病毒表达载体 |
| 终止子: | SV40 poly(A) signal |
| 复制子: | pUC |
| 启动子: | U6 |
| 载体大小: | 5141 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 绿色荧光ZsGreen1 |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pDC316-ZsGreen1-shRNA载体质粒基本信息
pDC316-ZsGreen1-shRNA质粒图谱载体图谱和pDC316-ZsGreen1-shRNA载体序列质粒序列多克隆位点信息
pDC316-ZsGreen1-shRNA质粒载体简介
pDC316-ZsGreen1-shRNA质粒序列载体序列
LOCUS pDC316-ZsGreen1-shRNA 5141 bp ds-DNA circular SYN 26-OCT-2017
DEFINITION synthetic circular DNA
KEYWORDS pDC316-ZsGreen1-shRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5141)
AUTHORS .
TITLE Direct Submission
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..5141
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 469..992
/label=MCMV promoter
CDS 1003..1698
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
polyA_signal 1836..1957
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1975..2215
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
polyA_signal 2264..2398
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 2418..2451
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(2875..3463)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3634..4494)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4495..4599)
/gene="bla"
/label=AmpR promoter
misc_feature 4682..453
/label=Ad
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg
481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc
721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct
901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggccca gtccaagcac
1021 ggcctgacca aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag
1081 ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg
1141 tgcgtggtgg agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg
1201 tacggcaacc gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc
1261 tgccccgccg gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc
1321 tgcaacgccg acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc
1381 tacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag
1441 ccctcctgcg agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc
1501 atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag
1561 gccaagtccg tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc
1621 gaggaccgca gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc
1681 ggctccgcct tgccctaaag atctcgagat ccaccggatc tagataactg atcataatca
1741 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
1801 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
1861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
1921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtctgggca ggaagagggc
1981 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
2041 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
2101 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
2161 accgtaactt gaaagtattt cgatttcttg ggtttatata tcttgtggaa aggacgaaac
2221 acctgcagtt ttttggatcc caatgtcgac ctcgacttcg agcaacttgt ttattgcagc
2281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
2341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgt
2401 ctagcatcga agatccaata acttcgtata gcatacatta tacgaagtta taagtagctt
2461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
2521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
2581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
2641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
2701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
2761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
2821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
2881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
2941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
3001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
3061 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
3121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
3181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
3241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
3301 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
3361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
3421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
3481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
3541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
3601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
3661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
3721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
3781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
3841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
3901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
3961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
4021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
4081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
4141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
4201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
4261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
4321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
4381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
4441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
4501 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
4561 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
4621 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
4681 tctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac agcggcagcc
4741 taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc
4801 accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac
4861 taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct
4921 acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc
4981 cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc
5041 gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac
5101 cccctcatta tcatattggc ttcaatccaa aataaggtat a
//
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