pER8

LOCUS       pER8               11517 bp ds-DNA     circular SYN 28-DEC-2017
DEFINITION  synthetic circular DNA
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        216..262
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             291..542
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             558..791
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             849..1742
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2294..2510
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2525..3550
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      3552..3804
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4127..4173
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4199..4237
                     /label=MCS
     misc_feature    4983..5007
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5535..6326
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=Spe
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6570..7158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7344..7484
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      7828..8022
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8088..9155)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(9589..10218)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
      121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
      181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
      241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
      301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
      361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
      421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
      481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
      541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
      721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
      781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
      841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
      901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
      961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
     1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
     1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
     1141 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
     1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
     1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
     1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
     1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
     1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
     1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
     1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
     1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
     1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
     1741 gagagctccc tggcgaattc ccagagatgt tagctgaaat catcactaat cagataccaa
     1801 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg
     1861 agtaagctag cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
     1921 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
     1981 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
     2041 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
     2101 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
     2161 gtaaaacatc ccaaacaagc ttggaaactg aaggcgggaa acgacaatct gatcatgagc
     2221 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
     2281 tttggaactg acagaaccgc aacgattgaa ggagccactc agccgcgggt ttctggagtt
     2341 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     2401 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     2461 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatcccc
     2521 tagaatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     2581 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     2641 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     2701 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     2761 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     2821 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     2881 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     2941 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     3001 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     3061 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     3121 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     3181 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     3241 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     3301 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     3361 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     3421 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     3481 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     3541 aaaggaatag cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     3601 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     3661 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     3721 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     3781 cggtgtcatc tatgttacta gatcggggaa ttgatccccc ctcgacagct tgcatgccag
     3841 cttgggctgc aggtcgaggc taaaaaacta atcgcattat catcccctcg acgtactgta
     3901 catataacca ctggttttat atacagcagt actgtacata taaccactgg ttttatatac
     3961 agcagtcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
     4021 accactggtt ttatatacag cagtcgaggt aagattagat atggatatgt atatggatat
     4081 gtatatggtg gtaatgccat gtaatatgct cgactctagg atcttcgcaa gacccttcct
     4141 ctatataagg aagttcattt catttggaga ggacacgctg aagctagtcg actctagcct
     4201 cgaggcgcgc cgggcccata cgcgttaatt aactagtcga tccaggcctc ccagctttcg
     4261 tccgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcccaca
     4321 caccagaatc ctactaagtt tgagtattat ggcattggaa aagctgtttt cttctatcat
     4381 ttgttctgct tgtaatttac tgtgttcttt cagtttttgt tttcggacat caaaatgcaa
     4441 atggatggat aagagttaat aaatgatatg gtccttttgt tcattctcaa attattatta
     4501 tctgttgttt ttactttaat gggttgaatt taagtaagaa aggaactaac agtgtgatat
     4561 taaggtgcaa tgttagacat ataaaacagt ctttcacctc tctttggtta tgtcttgaat
     4621 tggtttgttt cttcacttat ctgtgtaatc aagtttacta tgagtctatg atcaagtaat
     4681 tatgcaatca agttaagtac agtataggct ttttgtgtcg agggggtacc gagtcgagga
     4741 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4861 atcgcccttc ccaacagttg cgcagcctga atggcgggta ccgagctcga attcaattcg
     4921 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     4981 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     5041 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     5101 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     5161 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     5221 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctcgtt
     5281 cggttcgtaa gctgtaatgc aagtagcgta actgccgtca cgcaactggt ccagaacctt
     5341 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttctt gttatgacat
     5401 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     5461 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     5521 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     5581 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     5641 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     5701 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     5761 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     5821 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     5881 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     5941 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     6001 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     6061 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     6121 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     6181 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     6241 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     6301 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
     6361 ttcgccggcg ttaactcaag cgattagatg cactaagcac ataattgctc acagccaaac
     6421 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     6481 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6541 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6601 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6661 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6721 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6781 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6841 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6901 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6961 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7021 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7081 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7141 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7201 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7261 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7321 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     7381 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7441 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     7501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7621 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     7681 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     7741 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     7801 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     7861 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     7921 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     7981 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     8041 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     8101 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     8161 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     8221 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     8281 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     8341 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     8401 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     8461 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     8521 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     8581 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     8641 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     8701 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     8761 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     8821 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     8881 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     8941 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     9001 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     9061 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     9121 gcgccgattt gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg
     9181 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
     9241 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
     9301 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
     9361 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
     9421 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
     9481 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
     9541 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
     9601 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
     9661 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
     9721 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
     9781 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
     9841 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
     9901 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
     9961 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
    10021 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
    10081 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
    10141 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
    10201 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
    10261 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
    10321 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
    10381 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
    10441 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
    10501 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
    10561 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
    10621 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
    10681 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
    10741 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
    10801 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
    10861 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
    10921 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
    10981 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
    11041 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
    11101 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
    11161 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
    11221 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
    11281 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
    11341 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
    11401 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    11461 aaccttttca cgccctttta aatatccgtt attctaataa acgctctttt ctcttag
//