pCMV-Tag 3B

LOCUS Exported File 4327 bp ds-DNA circular SYN 28-十一月-2013 DEFINITION Mammalian expression vector for tagging proteins with an N-terminal Myc epitope.?For other reading frames, use pCMV-Tag 3A or pCMV-Tag 3C. ACCESSION . VERSION . KEYWORDS pCMV-Tag 3B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4327) AUTHORS Agilent Technologies TITLE Direct Submission JOURNAL Exported 2014年3月6日 from SnapGene Viewer 1.5.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4327 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 66..370 /label="CMV enhancer" /label="human cytomegalovirus immediate early enhancer" promoter 371..574 /label="CMV promoter" /label="human cytomegalovirus (CMV) immediate early promoter" promoter 620..638 /label="T3 promoter" /label="promoter for bacteriophage T3 RNA polymerase" CDS 676..678 /codon_start=1 /product="start codon" /label="ATG" /translation="M" CDS 679..708 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" misc_feature 710..783 /label="MCS" /label="multiple cloning site" misc_feature 791..801 /label="stop codons" /label="stop codons in all three reading frames" promoter complement(829..847) /label="T7 promoter" /label="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1121..1242 /label="SV40 poly(A) signal" /label="SV40 polyadenylation signal" rep_origin complement(1249..1704) /direction=LEFT /label="f1 ori" /label="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1731..1833 /gene="bla" /label="AmpR promoter" promoter 1837..2194 /label="SV40 promoter" /label="SV40 enhancer and early promoter" CDS 2229..3023 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label="NeoR/KanR" /label="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" misc_feature 3255..3302 /label="HSV TK poly(A) signal" /label="herpesvirus thymidine kinase polyadenylation signal" rep_origin 3631..4219 /direction=RIGHT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 661 gcggtggcgg ccgccatgga gcagaaactc atctctgaag aggatctgag cccgggcgga 721 tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg 781 cccggtacct taattaatta aggtaccagg taagtgtacc caattcgccc tatagtgagt 841 cgtattacaa ttcactcgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga 901 gatccaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta 961 gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt tcatgatcat 1021 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 1081 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 1141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 1201 gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg 1261 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 1321 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 1381 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 1441 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 1501 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 1561 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 1621 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 1681 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1741 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1801 gataaatgct tcaataatat tgaaaaagga agaatcctga ggcggaaaga accagctgtg 1861 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 1921 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 1981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 2041 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 2101 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 2161 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat 2221 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 2281 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 2341 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 2401 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 2461 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 2521 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 2581 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 2641 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 2701 tggacgaaga acatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 2761 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 2821 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 2881 atcaggacat agcgttggct acccgtgata ttgctgaaga acttggcggc gaatgggctg 2941 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 3001 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 3061 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 3121 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 3181 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 3241 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt 3301 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 3361 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 3421 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc 3481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 3541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 3601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 3661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 3721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 3781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 3841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 3901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 3961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 4021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 4081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 4141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 4201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 4261 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 4321 taccgcc //