| 出品公司: | Takara |
|---|---|
| 载体名称: | pTf16 |
| 质粒类型: | Chaperone Plasmid分子伴侣质粒 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | chloromycetin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTf16 5015 bp ds-DNA circular SYN 27-DEC-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5015)
AUTHORS biofeng.com
TITLE Direct Submission
JOURNAL Exported Saturday, Dec 30, 2023 from SnapGene 4.3.6
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5015
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1893..3191
/codon_start=1
/product="ribosome-associated chaperone from E. coli
(Hoffmann et al., 2010)"
/label=Trigger Factor
/translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGFR
KGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGEDFT
YSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEAEDR
VTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFPEEYH
AENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMERELKSA
IRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPRELFEEQ
AKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNKELMDNMR
NVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
rep_origin 3624..4168
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
promoter 4694..4796
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS join(4797..5015,1..441)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaag aatagttacg
541 gcttatgaca tctttgtgga cacatcattc actttttatt cacatccggc cctgaactcg
601 ctaggacttg ccccggtgca ttttttaaat acccgcgaaa aatagagctg atcgtcaaat
661 ccaacattgc gcccaacggt cgctatcggc attcgcgtag tgctaagcag aagtttcgcc
721 tggctgatac gctgatcttc gcgccagctc aatacgctaa tgcctaactg ctggcggaac
781 agatgtgata accgggaggg cgacaggcag acatgctggg cgacgctggc gatatcaaaa
841 tggctgtccg ccagatggtc gctgatatac tggcaggcat cgcgcacacg gctatccatc
901 ggcgggtgca acgactcatt aattaccgcc atacgtctga gcaacaactg ctccagcaga
961 ttgatcgcca gtagctcaga atagcgacct tccccttgcc cggcgctgat gatctgcccg
1021 aacagttcgc tgaaatgcgg ctggcgcgcc tcgtccgggc ggaaaaatcc tgtctgggca
1081 aagattgtcg gccaggtcag ccactcctgc cagtaggcgc gaggccggaa ataaacccac
1141 tggtgatacc actcgctggc gtccggatgc cgtccatagt gatgaatctc gcccggcgga
1201 aacaataata tatcgccagg ccgacagaca aactgctcgc cattattatt aatgacgccc
1261 tctccgcgga tggtcaggtt aagaatatat cccttcatgc ccaacggacg atcgataaaa
1321 aaatccagat atccattcgc ttcaattggc gtcagcccgg cgaccagatg ggcattaaat
1381 gaatatcccg gcaatagcgg atcattttgc gtttcagcca tgatttctct accccccgat
1441 gttcagagaa gaaacaaatt gtccatatcg accaggacga cagagcttcc gtctccgcaa
1501 gactttgcgc ttgatgaaag cacgtatcaa ccccgcttgt gaaaagcgct ttgtaacaaa
1561 agcgtacagt tcaggcgata aaattaagta acagaagtgt ctataactat ggctggaatg
1621 tccacattga atatttgcac agcgtcacac tttgcaaagc attagcattt ttgtccataa
1681 gattagcgga tcctgcctga cggtttttgc cgcgactctc tataatttct ccatacctgt
1741 ttttctggat ggagtaagac catggccttt cgggatgatt ctggtaacag ggaatgtgat
1801 tgattataag aacatcccgg ttccgcgaag ccaacaacct gtgcttgcgg ggtaagagtt
1861 gaccgagcac tgtgattttt tgaggtaaca agatgcaagt ttcagttgaa accactcaag
1921 gccttggccg ccgtgtaacg attactatcg ctgctgacag catcgagacc gctgttaaaa
1981 gcgagctggt caacgttgcg aaaaaagtac gtattgacgg cttccgcaaa ggcaaagtgc
2041 caatgaatat cgttgctcag cgttatggcg cgtctgtacg ccaggacgtt ctgggtgacc
2101 tgatgagccg taacttcatt gacgccatca ttaaagaaaa aatcaatccg gctggcgcac
2161 cgacttatgt tccgggcgaa tacaagctgg gtgaagactt cacttactct gtagagtttg
2221 aagtttatcc ggaagttgaa ctgcagggtc tggaagcgat cgaagttgaa aaaccgatcg
2281 ttgaagtgac cgacgctgac gttgacggca tgctggatac tctgcgtaaa cagcaggcga
2341 cctggaaaga aaaagacggc gctgttgaag cagaagaccg cgtaaccatc gacttcaccg
2401 gttctgtaga cggcgaagag ttcgaaggcg gtaaagcgtc tgatttcgta ctggcgatgg
2461 gccagggtcg tatgatcccg ggctttgaag acggtatcaa aggccacaaa gctggcgaag
2521 agttcaccat cgacgtgacc ttcccggaag aataccacgc agaaaacctg aaaggtaaag
2581 cagcgaaatt cgctatcaac ctgaagaaag ttgaagagcg tgaactgccg gaactgactg
2641 cagaattcat caaacgtttc ggcgttgaag atggttccgt agaaggtctg cgcgctgaag
2701 tgcgtaaaaa catggagcgc gagctgaaga gcgccatccg taaccgcgtt aagtctcagg
2761 cgatcgaagg tctggtaaaa gctaacgaca tcgacgtacc ggctgcgctg atcgacagcg
2821 aaatcgacgt tctgcgtcgc caggctgcac agcgtttcgg tggcaacgaa aaacaagctc
2881 tggaactgcc gcgcgaactg ttcgaagaac aggctaaacg ccgcgtagtt gttggcctgc
2941 tgctgggcga agttatccgc accaacgagc tgaaagctga cgaagagcgc gtgaaaggcc
3001 tgatcgaaga gatggcttct gcgtacgaag atccgaaaga agttatcgag ttctacagca
3061 aaaacaaaga actgatggac aacatgcgca atgttgctct ggaagaacag gctgttgaag
3121 ctgtactggc gaaagcgaaa gtgactgaaa aagaaaccac tttcaacgag ctgatgaacc
3181 agcaggcgta atttacgcag cataacgcgc taaattcgca caaaggcccg tcaccgccag
3241 gtggtgggct tttttttgtc atgaattttg catggaaccg tgcgaaaagc ctctttcggt
3301 gttagcgtaa caacaaaaga ttgttatgct tgaaatatgg tgatgccgta cccataacac
3361 agggactagc tgataatccg tccataaggt tacaatcggt acagcaggtt ttttcaattt
3421 tatccaggag acggaaatgt catacagcgg cgaacgagat aactttgcac cccatagatc
3481 tggtaccaag cttatcgatg ataagctgtc aaacatgaga attacaactt atatcgtatg
3541 gggctgactt caggtgctac atttgaagag ataaattgca ctgaaatcta gaaatatttt
3601 atctgattaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct
3661 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct
3721 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag
3781 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc
3841 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc
3901 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
3961 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
4021 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg gaaacgcctg
4081 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg
4141 cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctct cacttccctg
4201 ttaagtatct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct
4261 cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg
4321 tatcacatat tctgctgacg caccggtgca gccttttttc tcctgccaca tgaagcactt
4381 cactgacacc ctcatcagtg ccaacatagt aagccagtat acactccgct agcgctgatg
4441 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
4501 gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa atcagtaagt
4561 tggcagcatc acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg
4621 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
4681 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
4741 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
4801 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
4861 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
4921 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
4981 ttcttgcccg cctgatgaat gctcatccgg aattc
//
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