出品公司: | Clontech |
---|---|
载体名称: | pAbAi |
质粒类型: | 酵母单杂交系统 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | -- |
启动子: | -- |
载体大小: | 4894 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | URA3 |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pAbAi载体质粒基本信息
pAbAi质粒图谱载体图谱和pAbAi载体序列质粒序列多克隆位点信息
pAbAi质粒载体简介
pAbAi质粒序列载体序列
LOCUS pAbAi 4894 bp ds-DNA circular SYN 19-OCT-2017 DEFINITION Yeast integrating vector for cloning a DNA bait sequence to be used in a one-hybrid screen. ACCESSION . VERSION . KEYWORDS pAbAi SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4894) AUTHORS Clontech (TaKaRa) TITLE Direct Submission JOURNAL Exported Saturday, January 11, 2020 from SnapGene Viewer 3.1.4 http://www.snapgene.com COMMENT Linearize with BstBI or BbsI to integrate at the URA3 locus. FEATURES Location/Qualifiers source 1..4894 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" misc_feature 1..45 /note="MCS" /note="multiple cloning site" CDS 185..1390 /codon_start=1 /gene="S. cerevisiae AUR1 with a dominant F158Y mutation" /product="aureobasidin A-resistant mutant of inositol phosphorylceramide synthase" /note="AurR" /note="confers fungal resistance to Aureobasidin A" /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK RA" rep_origin complement(2047..2635) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2806..3666) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3667..3771) /gene="bla" /note="AmpR promoter" CDS complement(3875..4678) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /note="URA3" /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(4679..4894) /gene="S. cerevisiae URA3" /note="URA3 promoter" ORIGIN 1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat 61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc 121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt 181 aataatggca aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt 241 agccgattta gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa 301 acccgcttta agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt 361 cattactaat cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt 421 attcatcatt ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt 481 ggcgctgtat ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa 541 agtgttacca gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac 601 atcgacgaat tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg 661 ggccccattt gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg 721 ttatgctttt gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc 781 agccgctccc ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca 841 tggctcgcct ggtggattag ctagaattga taagctactc ggtattaata tgtatactac 901 atgtttttca aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc 961 tactatggaa gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc 1021 ttatgtttgc tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct 1081 tatggcaggt tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc 1141 aattgtagat acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat 1201 atcaaagagt gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa 1261 cttggaactt gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt 1321 tgatggatct acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa 1381 gagggcttaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt 1441 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg 1501 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct 1561 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac 1621 tctagcgaat taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct 1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 2701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 2761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 2941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 3421 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 3661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 3781 ccgaaaagtg ccacctgggt aataactgat ataattaaat tgaagctcta atttgtgagt 3841 ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca 3901 aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct 3961 ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca 4021 cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca 4081 taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga 4141 taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag 4201 atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta 4261 cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat 4321 tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg 4381 tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata 4441 atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg 4501 tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg 4561 tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct 4621 tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga 4681 tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat 4741 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc 4801 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc 4861 aaaaaaaaga ataaaaaaaa aatgatgaat tgaa //