| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pYES2 |
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5857 bp |
| 5' 测序引物及序列: | T7: TAATACGACTCACTATAGGG |
| 3' 测序引物及序列: | CYC1 Terminator: GTGACATAACTAATTACATGATG |
| 载体标签: | / |
| 载体抗性: | 氨苄 |
| 筛选标记: | URA3 |
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
| 产品目录号: | V825-20 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYES2载体质粒基本信息
pYES2质粒图谱载体图谱和pYES2载体序列质粒序列多克隆位点信息
pYES2质粒载体简介
pYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进行转化株的筛选。
该载体包含以下元素：
1. 酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达
2.多克隆位点可以使用的很多限制酶切位点，便于基因插入。
3.CYC1终止子能够有效终止mRNA的转录。
4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。
5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。
pYES2质粒序列载体序列
LOCUS pYES2 5857 bp DNA circular SYN
DEFINITION pYES2
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5857
/organism="pYES2"
/mol_type="other DNA"
terminator complement(12..251)
/label="CYC1_terminator"
misc_feature 218..236
/label="pYESTrp_rev_primer"
misc_feature 218..236
/label="CYC1_primer"
promoter complement(368..386)
/label="T7_promoter"
promoter complement(410..861)
/label="GAL1_promoter"
misc_feature complement(424..447)
/label="GAL1_primer"
rep_origin complement(1402..2873)
/label="2micron_origin"
rep_origin 1404..2283
/label="2micron2_origin"
promoter 2876..3101
/label="URA3_promoter"
gene 3103..3903
/label="URA3"
/gene="URA3"
CDS 3103..3906
/label="ORF frame 1"
CDS complement(3138..3614)
/label="ORF frame 3"
gene 4002..4862
/label="Ampicillin (variant)"
/gene="Ampicillin (variant)"
CDS 4002..4625
/label="ORF frame 3"
rep_origin 5017..5636
/label="pBR322_origin"
ORIGIN
1 GGCCGCAAAT TAAAGCCTTC GAGCGTCCCA AAACCTTCTC AAGCAAGGTT TTCAGTATAA
61 TGTTACATGC GTACACGCGT CTGTACAGAA AAAAAAGAAA AATTTGAAAT ATAAATAACG
121 TTCTTAATAC TAACATAACT ATAAAAAAAT AAATAGGGAC CTAGACTTCA GGTTGTCTAA
181 CTCCTTCCTT TTCGGTTAGA GCGGATGTGG GGGGAGGGCG TGAATGTAAG CGTGACATAA
241 CTAATTACAT GATGCGGCCC TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT
301 CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT CCGAGCTCGG TACCAAGCTT
361 AATATTCCCT ATAGTGAGTC GTATTACAGC TGCTAGTAGT CCGATCCGGG GTTTTTTCTC
421 CTTGACGTTA AAGTATAGAG GTATATTAAC AATTTTTTGT TGATACTTTT ATTACATTTG
481 AATAAGAAGT AATACAAACC GAAAATGTTG AAAGTATTAG TTAAAGTGGT TAATGCAGTT
541 TTTGCATTTA TATATCTGTT AATAGATCAA AAATCATCGC TTCGCTGATT AATTACCCCA
601 GAAATAAGGC TAAAAAACTA ATCGCATTAT CATCCTATGG TTGTTAATTT GATTCGTTCA
661 TTTGAAGGTT TGTGGGGCCA GGTTACTGCC AATTTTTCCT CTTCATAACC ATAAAAGCTA
721 GTATTGTAGA ATCTTTATTG TTCGGAGCAG TGCGGCGCGA GGCACATCTG CGTTTCAGGA
781 ACGCGACCGG TGAGGACGAG GACGCACGGA GGAGAGTCTT CCTTCGGAGG GCTGTCACCC
841 GCTCGGCGGC TTCTAATCCG TACTAGTGGA TCATCCCCAC GCGCCCTGTA GCGCCCCATT
901 AAGCGCGGCG GGTGTGGTGG TTACGCCCAG CGTGACCCCT ACACTTCCCA CCGCCCTAGC
961 CCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA
1021 AGCTCTAAAT GGGGGCATCC GTTTACCCTT CCGATTTACT GCTTTACGGC ACCTCGACCC
1081 CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACCCTTTT
1141 TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTGG AAACTGGAAC
1201 AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGG
1261 CTATTCGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT
1321 AACGTTTACA ATTTAAATAT TTGCTTATAC AATCTTCCTG TTTTTGGGGC TTTTCTGATT
1381 ATCAACCGGG GTGGAGCTTC CCATTGCGAA TACCGCTTCC ACAAACATTG CTCAAAAGTA
1441 TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC CTACCCATCC ACCTTTCGCT
1501 CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT GTTTATCTCT AGTATTACTC
1561 TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG AATCGAAAAC AATACGAAAA
1621 TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG AAGAAACCGT TCATAATTTT
1681 CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC ACAAAGTATG CGCAATCCAC
1741 ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA AAATGCACCC GCAGCTTCGC
1801 TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT TATGGAAGAG AAAATAGACA
1861 CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC AAAAAGTTAT CAAGAGACTG
1921 CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA TGCTTTGTTA GAAAAATAGC
1981 GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA GATTCTTTGT TGGTAAAATA
2041 GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA
2101 TTAGCGCTCT CGTCGCGTTG CATTTTTGTT TTACAAAAAT GAAGCACAGA TTCTTCGTTG
2161 GTAAAATAGC GCTTTCGCGT TGCATTTCTG TTCTGTAAAA ATGCAGCTCA GATTCTTTGT
2221 TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTCTACA AAATGAAGCA CAGATGCTTC
2281 GTTAACAAAG ATATGCTATT GAAGTGCAAG ATGGAAACGC AGAAAATGAA CCGGGGATGC
2341 GACGTGCAAG ATTACCTATG CAATAGATGC AATAGTTTCT CCAGGAACCG AAATACATAC
2401 ATTGTCTTCC GTAAAGCGCT AGACTATATA TTATTATACA GGTTCAAATA TACTATCTGT
2461 TTCAGGGAAA ACTCCCAGGT TCGGATGTTC AAAATTCAAT GATGGGTAAC AAGTACGATC
2521 GTAAATCTGT AAAACAGTTT GTCGGATATT AGGCTGTATC TCCTCAAAGC GTATTCGAAT
2581 ATCATTGAGA AGCTGCAGCG TCACATCGGA TAATAATGAT GGCAGCCATT GTAGAAGTGC
2641 CTTTTGCATT TCTAGTCTCT TTCTCGGTCT AGCTAGTTTT ACTACATCGC GAAGATAGAA
2701 TCTTAGATCA CACTGCCTTT GCTGAGCTGG ATCAATAGAG TAACAAAAGA GTGGTAAGGC
2761 CTCGTTAAAG GACAAGGACC TGAGCGGAAG TGTATCGTAC AGTAGACGGA GTATCTAGTA
2821 TAGTCTATAG TCCGTGGAAT TAATTCTCAT CTTTGACAGC TTATCATCGA TAAGCTAGCT
2881 TTTCAATTCA ATTCATCATT TTTTTTTTAT TCTTTTTTTT GATTTCGGTT TCTTTGAAAT
2941 TTTTTTGATT CGGTAATCTC CGAACAGAAG GAAGAACGAA GGAAGGAGCA CAGACTTAGA
3001 TTGGTATATA TACGCATATG TAGTGTTGAA GAAACATGAA ATTGCCCAGT ATTCTTAACC
3061 CAACTGCACA GAACAAAAAC CTGCAGGAAA CGAAGATAAA TCATGTCGAA AGCTACATAT
3121 AAGGAACGTG CTGCTACTCA TCCTAGTCCT GTTGCTGCCA AGCTATTTAA TATCATGCAC
3181 GAAAAGCAAA CAAACTTGTG TGCTTCATTG GATGTTCGTA CCACCAAGGA ATTACTGGAG
3241 TTAGTTGAAG CATTAGGTCC CAAAATTTGT TTACTAAAAA CACATGTGGA TATCTTGACT
3301 GATTTTTCCA TGGAGGGCAC AGTTAAGCCG CTAAAGGCAT TATCCGCCAA GTACAATTTT
3361 TTACTCTTCG AAGACAGAAA ATTTGCTGAC ATTGGTAATA CAGTCAAATT GCAGTACTCT
3421 GCGGGTGTAT ACAGAATAGC AGAATGGGCA GACATTACGA ATGCACACGG TGTGGTGGGC
3481 CCAGGTATTG TTAGCGGTTT GAAGCAGGCG GCAGAAGAAG TAACAAAGGA ACCTAGAGGC
3541 CTTTTGATGT TAGCAGAATT GTCATGCAAG GGCTCCCTAT CTACTGGAGA ATATACTAAG
3601 GGTACTGTTG ACATTGCGAA GAGCGACAAA GATTTTGTTA TCGGCTTTAT TGCTCAAAGA
3661 GACATGGGTG GAAGAGATGA AGGTTACGAT TGGTTGATTA TGACACCCGG TGTGGGTTTA
3721 GATGACAAGG GAGACGCATT GGGTCAACAG TATAGAACCG TGGATGATGT GGTCTCTACA
3781 GGATCTGACA TTATTATTGT TGGAAGAGGA CTATTTGCAA AGGGAAGGGA TGCTAAGGTA
3841 GAGGGTGAAC GTTACAGAAA AGCAGGCTGG GAAGCATATT TGAGAAGATG CGGCCAGCAA
3901 AACTAAAAAA CTGTATTATA AGTAAATGCA TGTATACTAA ACTCACAAAT TAGAGCTTCA
3961 ATTTAATTAT ATCAGTTATT ACCCATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC
4021 GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA
4081 CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC
4141 TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA
4201 TGAGCACTTT TAAAGTTCTG CTATGTGATA CACTATTATC CCGTATTGAC GCCGGGCAAG
4261 AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA
4321 CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA
4381 TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA
4441 CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC
4501 TGAATGAAGC CATACCAAAC GACGAGAGTG ACACCACGAT GCCTGTAGCA ATGCCAACAA
4561 CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG
4621 ACTGAATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT
4681 GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC
4741 TGGGGCCAGA TGGTAAGCGC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA
4801 CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT
4861 AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT
4921 TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG
4981 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
5041 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
5101 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
5161 CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
5221 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
5281 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
5341 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
5401 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
5461 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
5521 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
5581 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
5641 TTTTACGGTT CCTGGGCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
5701 CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC
5761 GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC
5821 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAG
//
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pYES2质粒载体应用举例
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