出品公司: | -- |
---|---|
载体名称: | pLVTH |
质粒类型: | 哺乳动物细胞慢病毒表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | EF-1 alpha |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 11086 bp |
5' 测序引物及序列: | EF-1a Forward: TCAAGCCTCAGACAGTGGTTC |
3' 测序引物及序列: | EGFP-N: CGTCGCCGTCCAGCTCGACCAG |
载体标签: | C-EGFP |
载体抗性: | 氨苄 |
备注: | 载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率。该载体已经被pLVTHM载体替代,pLVTHM载体允许shRNA的定向克隆。 |
产品目录号: | -- |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
买家导航
pLVTH载体质粒基本信息
pLVTH质粒图谱载体图谱和pLVTH载体序列质粒序列多克隆位点信息
pLVTH质粒载体简介
pLVTH质粒序列载体序列
LOCUS pLVTH 11086 bp DNA circular SYN DEFINITION pLVTH ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..11086 /organism="pLVTH" /mol_type="other DNA" misc_feature 455..635 /label="truncHIV-1_3_LTR" misc_feature 455..635 /label="HIV-1_5_LTR" misc_feature 746..790 /label="HIV-1_psi_pack" CDS 1178..2056 /label="ORF frame 2" misc_feature 1300..1533 /label="RRE" CDS complement(1906..3039) /label="ORF frame 2" promoter 2129..3299 /label="EF1a_promoter" misc_feature 3247..3267 /label="EF1a_fwd_primer" misc_feature 3351..3366 /label="cPPT" CDS 3554..4291 /label="ORF frame 2" gene 3557..4270 /label="EGFP" /gene="EGFP" misc_feature complement(3599..3620) /label="EGFP_N_primer" misc_feature 3737..3766 /label="Y66 (EGFP)" misc_feature 4207..4228 /label="EGFP_C_primer" misc_feature 4346..4933 /label="WPRE" CDS complement(4431..4934) /label="ORF frame 3" CDS 4434..4994 /label="ORF frame 3" misc_feature 5004..5019 /label="cPPT" misc_feature 5004..5025 /label="U3PPT" misc_feature 5086..5373 /label="TRE" misc_feature 5534..5553 /label="H1_primer" misc_feature 5648..5681 /label="loxP" misc_feature 5701..5881 /label="truncHIV-1_3_LTR" misc_feature 5701..5881 /label="HIV-1_5_LTR" misc_feature complement(5907..5924) /label="Sp6_primer" gene 6162..6425 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(6214..6233) /label="pBRrevBam_primer" misc_feature 6566..6588 /label="pGEX_3_primer" promoter 6747..6775 /label="AmpR_promoter" gene 6817..7677 /label="Ampicillin" /gene="Ampicillin" CDS 6817..7677 /label="ORF frame 1" rep_origin 7832..8451 /label="pBR322_origin" misc_feature complement(8677..8697) /label="pBABE_3_primer" misc_feature complement(8683..9136) /label="SV40_enhancer" promoter 8695..8963 /label="SV40_promoter" rep_origin 8862..8939 /label="SV40_origin" misc_feature 8924..8943 /label="SV40pro_F_primer" misc_feature 10144..10159 /label="SV40_int" misc_feature 10165..10212 /label="SV40_3_splice" CDS 10289..10762 /label="ORF frame 2" misc_feature 10340..10360 /label="SV40 NLS" misc_feature 10346..10360 /label="NLS" terminator 10788..10919 /label="SV40_PA_terminator" misc_feature 10876..10895 /label="EBV_rev_primer" ORIGIN 1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac 61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct 2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc 2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg 2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat 2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc 2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc 2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc 2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg 2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg 2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt 2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg 2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa 2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg 2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac 3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg 3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg 3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc 3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt 3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag 3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag 3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag 3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct 3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa 4321 gcttcgggga ctagtcatat gataatcaac ctctggatta caaaatttgt gaaagattga 4381 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 4441 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4501 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4561 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4621 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4681 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 4741 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 4801 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 4861 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 4921 ccgcctcccc gcatcggtac gtatggccag gtacctttaa gaccaatgac ttacaaggca 4981 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 5041 caacgaagac aagatgggat caattcacca tgctagtgga tccccctcga gtttaccact 5101 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga 5161 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt 5221 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga 5281 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc 5341 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcggggctgc aggaattcga 5401 acgctgacgt catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca 5461 cccagcgcgc gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg 5521 caatatttgc atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga 5581 tttgggaatc ttataagttc tgtatgagac cacagatctc caagcttatc gataccgtcg 5641 catgggaata acttcgtata gcatacatta tacgaagtta tgctgctttt tgcttgtact 5701 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5761 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 5821 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 5881 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac 5941 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg 6001 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg 6061 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca 6121 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg 6181 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg 6241 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag 6301 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg 6361 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg 6421 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 6481 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 6541 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 6601 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 6661 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 6721 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 6781 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 6841 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 6901 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 6961 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 7021 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 7081 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 7141 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 7201 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 7261 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 7321 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 7381 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 7441 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 7501 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 7561 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 7621 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 7681 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 7741 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 7801 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 7861 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 7921 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 7981 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 8041 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 8101 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 8161 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 8221 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac 8281 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 8341 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 8401 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 8461 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 8521 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 8581 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 8641 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg 8701 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 8761 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 8821 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 8881 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 8941 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 9001 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt 9061 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt 9121 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa 9181 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac 9241 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa 9301 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg 9361 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc 9421 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg 9481 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca 9541 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc 9601 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg 9661 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg 9721 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc 9781 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt 9841 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg 9901 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc 9961 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat 10021 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa 10081 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc 10141 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg 10201 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa 10261 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga 10321 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc 10381 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt 10441 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata 10501 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac 10561 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag 10621 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga 10681 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 10741 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 10801 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 10861 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca 10921 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca 10981 ctgccaatta cctagtggtt tcatttactc taaacctgtg attcctctga attattttca 11041 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtag //