LOCUS pLVX-TetOne-Luc 9935 bp ds-DNA circular SYN 24-SEP-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT 631844, 631845, 631846
FEATURES Location/Qualifiers
LTR 1..634
/note="5' LTR"
misc_feature 681..806
/note="Packaging signal"
misc_feature 1303..1536
/note="RRE"
misc_feature 2028..2144
/note="cPPT/CTS"
polyA_signal 2187..2321
/note="SV40 polyA signal"
CDS complement(2522..4174)
/label="luciferase"
/note="firefly luciferase"
promoter complement(4184..4548)
/note="TRE3GS promoter"
promoter 4568..5078
/note="hPGK promoter"
/note="human phosphoglycerate kinase 1 promoter"
CDS 5097..5843
/note="Tet-On 3G"
misc_feature 5857..6445
/note="WPRE"
LTR 6653..7286
/note="3' LTR"
rep_origin complement(7817..8402)
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8573..9433)
/note="AmpR"
promoter complement(9434..9538)
/note="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg
4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag
4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc
4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg
4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt
4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa
4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg
4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata
4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt
5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
5821 accttgacat gctccccggg taaacgcgtc tggaacaatc aacctctgga ttacaaaatt
5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
6241 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
6421 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
6481 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
6541 cacaagagga ggaggaggtg ggtttttcca gtcacacctc aggtaccttt aagaccaatg
6601 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
6661 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
6721 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
6781 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
6841 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
6901 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
6961 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
7021 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
7081 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
7141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
7201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
7261 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
7321 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac
7381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
7441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
7501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
7561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
8341 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
8401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
8461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
8521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
8581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
8641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
8701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
8761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
8821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
8881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
8941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
9001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
9061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
9121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
9181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
9241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
9301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
9361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
9421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
9481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
9541 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
9601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
9661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
9721 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
9781 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
9841 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat
9901 tgtatttgtt aaatatgtac tacaaactta gtagt
//