| 出品公司: | Addgene |
|---|---|
| 载体名称: | pPRIME-TREX-GFP-FF3 |
| 质粒类型: | 慢病毒载体；RNA干扰载体；四环素诱导载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | TREX |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8600bp |
| 5' 测序引物及序列: | EGFP-C Foward: CATGGTCCTGCTGGAGTTCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素（Chloramphenicol） |
| 筛选标记: | EGFP |
| 克隆宿主菌: | Stbl3，在30度条件下进行质粒扩增。 |
| 备注: |
3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive
promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase |
| 产品目录号: | 11667 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pPRIME-TREX-GFP-FF3载体质粒基本信息
pPRIME-TREX-GFP-FF3质粒图谱载体图谱和pPRIME-TREX-GFP-FF3载体序列质粒序列多克隆位点信息
pPRIME-TREX-GFP-FF3质粒载体简介
pPRIME-TREX-GFP-FF3质粒序列载体序列
LOCUS pPRIME-TREX-GFP-FF3 8600 bp DNA SYN
DEFINITION pPRIME-TREX-GFP-FF3
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8600
/organism="pPRIME-TREX-GFP-FF3"
/mol_type="other DNA"
promoter 239..803
/label="CMV_immearly_promoter"
misc_feature 318..605
/label="CAG_enhancer"
misc_feature 772..792
/label="CMV_fwd_primer"
misc_feature 820..976
/label="truncHIV-1_3_LTR"
misc_feature 820..976
/label="HIV-1_5_LTR"
misc_feature 1087..1131
/label="HIV-1_psi_pack"
CDS 1523..2410
/label="ORF frame 2"
misc_feature 1645..1878
/label="RRE"
misc_feature 2409..2424
/label="cPPT"
promoter 2606..3182
/label="CMV_immearly_promoter"
misc_feature 2685..2972
/label="CAG_enhancer"
misc_feature 3139..3159
/label="CMV_fwd_primer"
promoter 3140..3263
/label="CMV_promoter"
misc_feature 3190..3229
/label="tetO"
misc_feature 3239..3263
/label="LNCX_primer"
CDS 3302..4021
/label="ORF frame 2"
gene 3305..4018
/label="EGFP"
/gene="EGFP"
misc_feature complement(3347..3368)
/label="EGFP_N_primer"
misc_feature 3485..3514
/label="Y66 (EGFP)"
misc_feature 3955..3976
/label="EGFP_C_primer"
gene 4546..5205
/label="CAT/CamR"
/gene="CAT/CamR"
CDS 4546..5205
/label="ORF frame 1"
misc_feature 5491..6078
/label="WPRE"
CDS 5579..6133
/label="ORF frame 2"
CDS 5592..6632
/label="ORF frame 3"
misc_feature complement(6081..6097)
/label="pBluescriptKS_primer"
misc_feature 6269..6284
/label="cPPT"
misc_feature 6269..6290
/label="U3PPT"
misc_feature 6606..6786
/label="HIV-1_5_LTR"
misc_feature 6606..6786
/label="truncHIV-1_3_LTR"
gene complement(7607..8467)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(7607..8467)
/label="ORF frame 2"
promoter complement(8509..8537)
/label="AmpR_promoter"
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata gaccagatct gagcctggga gctctctggc
841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
961 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
1021 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
1081 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
1141 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
1201 cagggggaaa gaaaaaatat aaataaacat atagtatggg caagcaggga gctagaacga
1261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
1321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
1381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
1441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat
1501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
1561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
1621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
1681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
1741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
1801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
1861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
1921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
1981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
2041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
2101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
2161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
2221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
2281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
2341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
2401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
2461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
2521 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga
2581 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
2641 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
2701 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2761 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2821 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2881 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2941 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
3001 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
3061 ttgacgtcaa tgggagtttg ttttggaacc aaaatcaacg ggactttcca aaatgtcgta
3121 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
3181 gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
3241 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgttac cggtcgccac
3301 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
3361 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
3421 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
3481 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
3541 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
3601 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
3661 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
3721 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
3781 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
3841 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
3901 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
3961 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
4021 gcggccgcaa gccttgttaa gtgctcgctt cggcagcaca tatactatgt ttgaatgagg
4081 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
4141 ttcaggttaa cccaacagaa ggctcgagca accagaattc aaggggctac tttaggagca
4201 attatcttgt ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct
4261 gaattaaaat ggtataaatt aaatcacttt tttcaattgg aagactaatg cgtttaaaca
4321 cgcggcgacg cgttcgaccg aataaaacct gtgacggaag atcacttcgc agaataaata
4381 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
4441 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
4501 gtattttttg agttgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
4561 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
4621 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
4681 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
4741 ctgatgaatg ctcatccgga attacgtatg gcaatgaaag acggtgagct ggtgatatgg
4801 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
4861 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
4921 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
4981 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
5041 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
5101 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
5161 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
5221 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
5281 ggcagaaatt cggatctcga ccgcgtttgg gcggtggctc cctgccacgc ggctccgaac
5341 agaagctgat ctccgaagag gatctgacat gtgtttaaac ctcgacttaa ttaagtcgag
5401 ggtcgacggt atcgataagc tcgcttcacg agatcatgtt taagggttcc ggttccacta
5461 ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
5521 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
5581 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
5641 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
5701 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
5761 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
5821 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
5881 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
5941 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
6001 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
6061 ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag
6121 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
6181 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
6241 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
6301 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
6361 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
6421 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
6481 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
6541 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
6601 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
6661 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
6721 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
6781 ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
7261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
7381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
7441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
7501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
7561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
7621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
7681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
7741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
7801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
7861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
7921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
7981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
8041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
8101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
8161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
8221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
8281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
8341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
8401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
8461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
8521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
8581 cccgaaaagt gccacctgac
//
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