| 出品公司: | Clontech |
|---|---|
| 载体名称: | pMSCVneo, pMSCV-neo, pMSCV neo |
| 质粒类型: | 哺乳动物细胞逆病毒基因表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | LTR |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6436bp |
| 5' 测序引物及序列: | MSCV-F: CCCTTGAACCTCCTCGTTCGACC |
| 3' 测序引物及序列: | pMSCV-R: GAGACGTGCTACTTCCATTTGTC |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | 新霉素（Neomycin） |
| 备注: | 逆病毒表达载体，优化后，有利于在造血干细胞中和癌细胞中进行基因表达。 |
| 产品目录号: | 634401 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
pMSCVneo载体 pMSCV-Neo质粒基本信息
pMSCVneo质粒图谱 pMSCV-Neo载体图谱和pMSCVneo载体序列 pMSCV-Neo质粒序列多克隆位点信息
pMSCVneo质粒 pMSCV-Neo载体简介
pMSCVneo质粒序列 pMSCV-Neo载体序列
LOCUS pMSCVneo 6436 bp DNA circular 4-DEC-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 634401,631461
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_signal 516..1405
/label=Extended\packaging\signal
promoter 1429..1937
/label=Phosphoglycerate\Kinase\Promoter
CDS 1951..2751
/label=Neo(R)
LTR 2859..3342
/label=3'\PCMV\LTR
LTR 1..515
/label=5'\MMSV\LTR
CDS complement(4645..5502)
/label=Amp(R)
rep_origin 3882..3882
/label=pUC\origin
splicing_signal 519..522
/label=Splice\Donor
splicing_signal 1397..1399
/label=Splice\Acceptor
BASE COUNT 1458 a 1758 c 1708 g 1512 t
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaattc gttaacctcg agagatctaa ttctaccggg
1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
1921 catctccggg cctttcgacc tgcagccaat atgggatcgg ccattgaaca agatggattg
1981 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
2041 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
2101 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
2161 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
2221 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
2281 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
2341 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
2401 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
2461 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
2521 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
2581 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
2641 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
2701 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat
2761 ccgtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa
2821 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
2881 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
2941 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
3001 gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat
3061 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
3121 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
3181 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg
3241 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
3301 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga
3361 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag
3421 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag
3481 aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc
3541 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
3601 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
3661 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
3721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
4561 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4621 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4741 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4801 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4921 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
4981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
5041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
5101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
5161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
5221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
5281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
5341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
5401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
5461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
5521 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
5581 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
5641 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
5701 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
5761 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
5821 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
5881 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
5941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
6001 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
6061 gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg
6121 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
6181 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
6241 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta
6301 gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat
6361 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
6421 cagaaaaagg ggggaa
//
