出品公司: | Addgene |
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载体名称: | pCS2+8CeGFP |
质粒类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | simian CMV IE94、lac、T3、SP6 |
载体大小: | 4854 bp |
5' 测序引物及序列: | SP6 fwd(MCS1)、T3 fwd(MCS2) |
3' 测序引物及序列: | EBV rev(MCS1)、SV40 polyA rev(MCS2) |
载体标签: | GFP |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCS2+8CeGFP载体能够在多种细胞中进行高水平的瞬表达。pCS2+8CeGFP载体同时也能进行体外转录和翻译。 |
产品目录号: | #34952 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCS2+8CeGFP
价格:1100元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCS2+8CeGFP Sequencing Result 4854 bp DNA SYN DEFINITION pCS2+8CeGFP Sequencing Result ACCESSION COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pCS2+8CeGFP| FEATURES Location/Qualifiers source 1..4854 /organism="pCS2+8CeGFP Sequencing Result" /mol_type="other DNA" gene complement(921..1781) /label="Ampicillin" /gene="Ampicillin" CDS complement(921..1781) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(1823..1851) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 2347..2871 /label="ORF frame 1" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" misc_feature 3340..3381 /label="CMV_fwd_primer" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" misc_feature 3457..3474 /label="Sp6_primer" /translation="MRRYRARPIRHSGCATVGKGDRCGPLRYYASRPPIQYGVYGLMP IQYGGSGPVPIQYGVYGLVPIQYGGSGPQPIQYGGLGTMPIQYGGPGTVPTGEGSTWH GAKFEEGSWPCAKSAILNWHGANNGGHIGYMPGSIYRQYPIWPYANMAIGQVQYYVLA LCHIVFHIWVFLLT*" CDS 3561..4277 /label="ORF frame 3" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" gene 3567..4274 /label="GFP (variant)" /gene="GFP (variant)" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" misc_feature complement(3591..3619) /label="GFP_R_primer" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" misc_feature complement(3635..3654) /label="GFPfusionREV_primer" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" misc_feature 3741..3770 /label="Y66 (EGFP)" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" misc_feature 4214..4234 /label="GFP_F_primer" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" misc_feature complement(4352..4371) /label="EBV_rev_primer" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" promoter complement(4574..4593) /label="T3_promoter" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" promoter complement(4610..4628) /label="M13_reverse_primer" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" misc_feature complement(4627..4649) /label="M13_pUC_rev_primer" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITHGMDELYK*" ORIGIN 1 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaAGGCGGTA 61 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 121 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 181 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 241 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 301 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 361 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 421 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 481 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 541 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 601 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 661 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 721 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 781 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 841 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 901 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 961 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 1021 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT 1081 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 1141 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 1201 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 1261 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 1321 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 1381 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 1441 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 1501 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 1561 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 1621 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 1681 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 1741 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 1801 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 1861 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA 1921 GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC 1981 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA 2041 GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG 2101 GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT 2161 TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA 2221 GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG 2281 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG 2341 CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG 2401 AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGTC GACCGCCAAT TCAATATGGC 2461 GTATATGGAC TCATGCCAAT TCAATATGGT GGATCTGGAC CTGTGCCAAT TCAATATGGC 2521 GTATATGGAC TCGTGCCAAT TCAATATGGT GGATCTGGAC CCCAGCCAAT TCAATATGGC 2581 GGACTTGGCA CCATGCCAAT TCAATATGGC GGACCTGGCA CTGTGCCAAC TGGGGAGGGG 2641 TCTACTTGGC ACGGTGCCAA GTTTGAGGAG GGGTCTTGGC CCTGTGCCAA GTCCGCCATA 2701 TTGAATTGGC ATGGTGCCAA TAATGGCGGC CATATTGGCT ATATGCCAGG ATCAATATAT 2761 AGGCAATATC CAATATGGCC CTATGCCAAT ATGGCTATTG GCCAGGTTCA ATACTATGTA 2821 TTGGCCCTAT GCCATATAGT ATTCCATATA TGGGTTTTCC TATTGACGTA GATAGCCCCT 2881 CCCAATGGGC GGTCCCATAT ACCATATATG GGGCTTCCTA ATACCGCCCA TAGCCACTCC 2941 CCCATTGACG TCAATGGTCT CTATATATGG TCTTTCCTAT TGACGTCATA TGGGCGGTCC 3001 TATTGACGTA TATGGCGCCT CCCCCATTGA CGTCAATTAC GGTAAATGGC CCGCCTGGCT 3061 CAATGCCCAT TGACGTCAAT AGGACCACCC ACCATTGACG TCAATGGGAT GGCTCATTGC 3121 CCATTCATAT CCGTTCTCAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCACTTGG 3181 CAGTACATCA ATATCTATTA ATAGTAACTT GGCAAGTACA TTACTATTGG AAGTACGCCA 3241 GGGTACATTG GCAGTACTCC CATTGACGTC AATGGCGGTA AATGGCCCGC GATGGCTGCC 3301 AAGTACATCC CCATTGACGT CAATGGGGAG GGGCAATGAC GCAAATGGGC GTTCCATTGA 3361 CGTAAATGGG CGGTAGGCGT GCCTAATGGG AGGTCTATAT AAGCAATGCT CGTTTAGGGA 3421 ACCGCCATTC TGCCTGGGGA CGTCGGAGCA AGCTTGATTT AGGTGACACT ATAGAATACA 3481 AGCTACTTGT TCTTTTTGCA GGATCCACTA GTGGCGCGCC ATTAATTAAA GGCCGGCCAG 3541 CGATCGCCGG ACATCGATTG ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA 3601 TTCTTGTTGA ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG 3661 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT GGAAAACTAC 3721 CTGTTCCATG GCCAACACTT GTCACTACTC TCACTTATGG TGTTCAATGC TTTTCAAGAT 3781 ACCCAGATCA TATGAAACGG CATGACTTTT TCAAGAGTGC CATGCCCGAA GGTTATGTAC 3841 AGGAAAGAAC TATATTTTTC AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT 3901 TTGAAGGTGA TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG 3961 GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC ACACAATGTA TACATCATGG 4021 CAGACAAACA AAAGAATGGA ATCAAAGTTA ACTTCAAAAT TAGACACAAC ATTGAAGATG 4081 GAAGCGTTCA ACTAGCAGAC CATTATCAAC AAAATACTCC AATTGGCGAT GGCCCTGTCC 4141 TTTTACCAGA CAACCATTAC CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA 4201 AGAGAGACCA CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG 4261 ATGAACTATA CAAATAAGAA TTCAAGGCCT CTCGAGCCTC TAGAACTATA GTGAGTCGTA 4321 TTACGTAGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT 4381 GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT 4441 TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA 4501 GGGGGAGGTG TGGGAGGTTT TTTAATTCGC GGCCGCGGCG CCAATGCATT GGGCCCGGTA 4561 CCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT TGGCGTAATC ATGGTCATAG 4621 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGGAGC 4681 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 4741 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 4801 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACT //
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