| 载体名称: | pKM101 |
|---|---|
| 来源历史: | <- NCIMB <- M. Green, Univ. Sussex, Brighton |
| 载体大小: | 35.4 kb |
| 质粒抗性: |
氨苄
|
| 载体说明: | pKM101是一种天然质粒（R-Brighton）的突变体。与Ames试验中使用的质粒相同。自我传播。抗紫外线。独特的限制性位点：EcoRI，BamHI。用于OECD化学品测试指南，“遗传毒理学：大肠杆菌，反向突变试验”（1983年5月）。有关更多信息，请参阅DSM 9494和DSM 9495。 |
| 宿主菌： | Escherichia coli WP2 (derivative of strain B) |
| 培养条件： | LB培养基，37度 |
| 参考文献： |
MacPhee, D. G. (1973). Salmonella typhimurium hisG46 (R-Utrecht): possible use in screening mutagens and carcinogens. Appl.Microbiol. 26 : 1004-1005 . Ames, B. N., McCann, J., Yamasaki, E. (1975). Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutation Research 31 : 347-364 . Green, M. H. L., (1984). Mutagen testing trp reversion in Escherichia coli. In Handbook of mutagenicity test procedures , pp. 161-187. Edited by B. J. Kilbey, Elsevier. Green, M. H. L., Muriel, W. J. (1976). Mutagen testing using Trp+ reversion in Escherichia coli. Mutation Research 38 : 3-32 . Maron, D. M., Ames, B. N., (1984). Revised methods for the Salmonella mutagenicity test. In Handbook of mutagenicity test procedures , pp. 93-140. Elsevier. Maron, D. M., Ames, B. N. (1984). Revised methods for the Salmonella mutagenicity test. Mutat Res. 113 : 173-215 . Mortelmans, K. E., Stocker, B. A. D. (1976). Ultraviolet light protection, enhancement of ultraviolet light mutagenesis and mutator effect of plasmid R46 in Salmonella typhimurium. J.Bacteriol. 128 : 271-282 . Mortelmans, K. E., Stocker, B. A. D. (1979). Segregation of the mutator property of plasmid R46 from its ultraviolet protecting property. Mol.Gen.Genet. 167 : 317-328 . |
| 安全等级： | 1级 |
| 分离国家: | 原产国未知 |
| 存储时间: | 1995年8月17日之前 |
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