载体名称: | pWW20 |
---|---|
来源历史: | <- P.A. Williams |
载体大小: | 270 - 280 kb |
质粒抗性: |
TOL
|
载体说明: | 天然存在的TOL质粒,在苯甲酸盐上生长后分离不寻常的缺失突变体;降解甲苯、间二甲苯和对二甲苯及其代谢物的代码;调节甲苯分解代谢途径的第一个工作模式。 |
宿主菌: | Pseudomonas putida MT20 |
培养条件: | LB培养基,28度 |
参考文献: |
Pickup, R. W., Williams, P. A. (1982). Spontaneous deletions in the TOL plasmid pWW20 which give rise to the B3 regulatory mutants of Pseudomonas putida MT20. J.Gen.Microbiol. 128 : 1385-1390 . Williams, P. A., Worsey, M. J. (1976). Ubiquity of plasmids in coding for toluene and xylene metabolism in soil bacteria: evidence for the existence of new TOL plasmids. J.Bacteriol. 125 : 818-828 . Worsey, M. J., Williams, P. A. (1977). Characterization of a spontaneously occurring mutant of the TOL20 plasmid in Pseudomonas putida MT20: possible regulatory implications. J.Bacteriol. 130 : 1149-1158 . |
安全等级: | 1级 |
分离国家: | 原产国未知 |
存储时间: | 1990年3月16日前 |