pCambia1391

LOCUS       pCAMBIA1391          10658 bp ds-DNA    circular SYN 16-四月-2013
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            hygromycin- and kanamycin-resistance genes and GUS lacking a start 
            codon.
ACCESSION   AF234308
VERSION     .
KEYWORDS    pCAMBIA1391
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     The GenBank record was corrected by inserting a G at position 4541.
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="synthetic DNA construct"
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
     CDS             20..1825
                     /codon_start=1
                     /gene="uidA?(or gusA)?from E. coli"
                     /product="beta-glucuronidase"
                     /note="GUS"
     CDS             1832..1849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      1881..2133
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2155..2179
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3479..4108
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
     CDS             4537..5610
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /note="
                     "
                     /protein_id="
                     "
     misc_feature    6214..6354
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6540..7128)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7215..8009)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /protein_id="
                     "
     misc_feature    8434..8458
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8536..8710
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8750..9775)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
     promoter        complement(9843..10520)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    10609..10644
                     /note="MCS"
                     /note="multiple cloning site from pUC9"
ORIGIN
        1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
       61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
      121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
      181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
      241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
      301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
      361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
      421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
      481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
      541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
      601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
      661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
      721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
      781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
      841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
      901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
      961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
     1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
     1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
     1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
     1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
     1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
     1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
     1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
     1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
     1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
     1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
     1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
     1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
     1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
     1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg
     1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag
     2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
     2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
     4501 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     4561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     4621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     4681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     4741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     4801 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     4861 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     4921 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     4981 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     5041 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     5101 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     5161 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     5221 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     5281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     5341 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     5401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     5461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     5521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     5581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     5641 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     5701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     5761 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     5821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     5881 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     5941 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     6001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     6061 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6121 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6181 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     6241 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     6361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6961 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7201 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
     7261 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
     7321 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
     7381 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
     7441 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
     7501 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
     7561 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
     7621 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
     7681 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
     7741 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
     7801 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
     7861 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
     7921 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
     7981 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
     8041 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     8101 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     8161 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
     8221 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     8281 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     8341 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     8401 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     8461 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     8521 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     8581 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     8641 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
     8701 tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg
     8761 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     8821 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     8881 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     8941 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg
     9001 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     9061 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     9121 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     9181 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     9241 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     9301 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     9361 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     9421 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     9481 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt
     9541 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     9601 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     9661 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     9721 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
     9781 attgcccccc gggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     9841 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag
     9901 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     9961 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
    10021 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
    10081 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
    10141 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
    10201 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
    10261 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
    10321 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
    10381 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
    10441 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
    10501 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
    10561 tggagtagac gagagtgtcg tgctccacca tgttgggccc ggcgcgccaa gcttggctgc
    10621 aggtcgacgg atccccggga attctaagag gagtccac
//