pCambia2301

LOCUS       AF234316               11633 bp    DNA     circular SYN 24-APR-2000
DEFINITION  Binary vector pCAMBIA-2301, complete sequence.
ACCESSION   AF234316
VERSION     AF234316.1  GI:7638149
KEYWORDS    .
SOURCE      Binary vector pCAMBIA-2301
  ORGANISM  Binary vector pCAMBIA-2301
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors
            for plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
   PUBMED   7919218
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     A comprehensive set of modular vectors for advanced manipulations
            and efficient transformation of plants
  JOURNAL   Unpublished
  REMARK    Full description of constructs
REFERENCE   3  (bases 1 to 11633)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
            GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Binary vector pCAMBIA-2301"
                     /mol_type="other DNA"
                     /db_xref="taxon:118408"
     CDS             join(2..16,207..2054)
                     /codon_start=1
                     /product="GUSA, hexaHis tagged"
                     /protein_id="AAF65404.1"
                     /db_xref="GI:7638153"
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE
                     SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
                     GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
                     NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
                     DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR
                     VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY
                     RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET
                     QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT
                     CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI
                     IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ
                     GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"
     exon            2..16
                     /note="synthetic first gus exon (modular)"
     intron          17..206
                     /note="Castorbean catalase intron; modified"
     exon            207..2054
                     /note="second gusA exon, His6"
     misc_feature    2086..2338
                     /note="nos (nopaline synthase) 3'UTR (polyA signal)"
     misc_feature    2376..2401
                     /note="right border T-DNA repeat"
     misc_feature    complement(3442..4442)
                     /note="STA region from pVS1 plasmid"
     rep_origin      complement(5035..6035)
                     /note="pVS1-REP; replication origin from pVS1"
     misc_feature    complement(6445..6705)
                     /note="bom site from pBR322"
     rep_origin      complement(6845..7125)
                     /note="pBR322 origin of replication"
     CDS             complement(7416..8210)
                     /note="aadA (kanamycin resistance) gene amplified from
                     pIG121Hm"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAF65402.1"
                     /db_xref="GI:7638151"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8635..8660
                     /note="left border repeat from C58 T-DNA"
     misc_feature    8727..8930
                     /note="CaMV 3'UTR (polyA signal)"
     CDS             complement(8968..9765)
                     /note="nptII (kanamycin resistance) gene"
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /protein_id="AAF65403.1"
                     /db_xref="GI:7638152"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(9796..10576)
                     /note="CaMV35S2; CaMV 35S promoter, duplicated"
     promoter        10724..10778
                     /note="PlacZ; lacZ promoter"
     CDS             10806..11039
                     /codon_start=1
                     /product="LacZ alpha fragment"
                     /protein_id="AAF65401.1"
                     /db_xref="GI:7638150"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    10820..10876
                     /note="pUC18 MCS; polylinker"
     promoter        11090..11627
                     /note="CaMV35S; 35S promoter from CaMV"
ORIGIN      
        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
     7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
     7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
     7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
     7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
     7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
     7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
     7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
     7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
     7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
     8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
     8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
     8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
     8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
     8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
     8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
     8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
     8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
     8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
     8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
     8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
     8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
     8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     8881 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac tctagctaga
     8941 ggatcgatcc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     9001 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     9061 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     9121 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     9181 cggcaagcag gcatcgccat gtgtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
     9241 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     9301 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     9361 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     9421 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     9481 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     9541 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tggagttcat tcagggcacc
     9601 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     9661 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     9721 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc cccatggtcg atcgacagat
     9781 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     9841 tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg
     9901 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
     9961 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
    10021 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
    10081 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
    10141 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
    10201 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc
    10261 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    10321 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
    10381 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
    10441 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
    10501 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
    10561 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
    10621 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    10681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    10741 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    10801 cagctatgac catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct
    10861 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    10921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    10981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
    11041 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc atggagtcaa
    11101 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
    11161 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
    11221 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
    11281 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
    11341 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
    11401 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    11461 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    11521 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    11581 cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt gac
//