| 出品公司: | -- |
|---|---|
| 载体名称: | pHDE-35S-Cas9-mCherry 载体 |
| 质粒类型: | 植物表达载体,CRISPR/CAS9系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 35S |
| 载体大小: | 15443 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 大观霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pHDE-35S-Cas9-mCherry载体质粒基本信息
pHDE-35S-Cas9-mCherry质粒图谱载体图谱和pHDE-35S-Cas9-mCherry载体序列质粒序列多克隆位点信息
pHDE-35S-Cas9-mCherry质粒载体简介
pHDE-35S-Cas9-mCherry质粒序列载体序列
LOCUS pHDE-35S-Cas9-mCherry 15443 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15443)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..15443
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..25
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
terminator 1203..1455
/label=MAS terminator
/note="mannopine synthase terminator"
promoter 1927..2106
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 1959..2142
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
terminator 3257..3509
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 3642..3987
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 3992..8095
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8108..8128
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(8640..8656)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 8878..8902
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 9430..10221
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 10465..11053
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 11239..11379
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 11723..11917
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(11983..13050)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
CDS complement(13484..14113)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactagtgaa gtgaagcttg
61 gtctcaacta tgagacctaa gctggcacaa ctatatttcc aacatcacta gctaccatca
121 aaagattgac ttctcatctt actcgattga aaccaaatta acatagggtt tttatttaaa
181 taaaagttta accttctttt taaaaaattg ttcatagtgt catgtcagaa caagagctac
241 aaatcacaca tagcatgcat aagcggagct atgatgagtg gtattgtttt gttcgtcact
301 tgtcactctt ttccaacaca taatcccgac aacaacgtaa gagcatctct ctctctccac
361 acacactcat gcatgcatgc attcttacac gtgattgcca tgcaaatctc ctttctcacc
421 tataaataca aaccaaccct tcactacact cttcactcaa accaaaacaa gaaaacatac
481 acaaatagca aaacggtacc aacaatggat aacatggcca tcatcaagga gttcatgcgc
541 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
601 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
661 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
721 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
781 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
841 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
901 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
961 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
1021 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
1081 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
1141 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgagag
1201 ctcttggact cccatgttgg caaaggcaac caaacaaaca atgaatgatc cgctcctgca
1261 tatggggcgg tttgagtatt tcaactgcca tttgggctga attgaagaca tgctcctgtc
1321 agaaattccg tgatcttact caatattcag taatctcggc caatatccta aatgtgcgtg
1381 gctttatctg tctttgtatt gtttcatcaa ttcatgtaac gtttgctttt cttatgaatt
1441 ttcaaataaa ttatcgtatt gagaccgaat tctgtctaga cctaactgag taagctagct
1501 tgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
1561 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
1621 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
1681 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
1741 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactag
1801 atccccaaac aagcttgaaa ctgaaggcgg gaaacgacaa tctgatcatg agcggagaat
1861 taagggagtc acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa
1921 ctgacagaac cgcaacgttg aaggagccac tcagccgcgg gtttctggag tttaatgagc
1981 taagcacata cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag
2041 ctagcaaata tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc
2101 caattagagt ctcatattca ctctcaatcc aaataatctg caccggatcc gctagaggat
2161 ctcgacctgc aagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
2221 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
2281 gagggcgaag aatctcgtgc tttcagcttc gatataggag ggcgtggata tgtcctgcgg
2341 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
2401 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
2461 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
2521 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
2581 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
2641 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
2701 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
2761 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
2821 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
2881 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
2941 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
3001 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
3061 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
3121 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
3181 aaccgacgcc ccagcactcg tccgggatct tggaggtgat gtaacatgat cacaagctga
3241 tcccccgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
3301 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
3361 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
3421 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
3481 gcgcgcggtg tcatctatgt tactagatcg ggaattgatc ccccctcgac agcttgcatg
3541 ccagcttggg ctgcaggtcg acgcgtcaac atggtggagc acgacactct cgtctactcc
3601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
3661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
3721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
3781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
3841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
3901 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
3961 ggaagttcat ttcatttgga gaggacacga tatggacaag aagtactcca ttgggctcga
4021 tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg tgccgagcaa
4081 aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc tcattggcgc
4141 cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa cagcacggcg
4201 cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta gtaatgagat
4261 ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg tggaggagga
4321 taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg cgtaccatga
4381 aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg ataaggctga
4441 cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac acttcctcat
4501 cgagggggac ctgaacccag acaacagcga tgtcgacaaa ctctttatcc aactggttca
4561 gacttacaat cagcttttcg aagagaaccc gatcaacgca tccggagttg acgccaaagc
4621 aatcctgagc gctaggctgt ccaaatcccg gcggctcgaa aacctcatcg cacagctccc
4681 tggggagaag aagaacggcc tgtttggtaa tcttatcgcc ctgtcactcg ggctgacccc
4741 caactttaaa tctaacttcg acctggccga agatgccaag cttcaactga gcaaagacac
4801 ctacgatgat gatctcgaca atctgctggc ccagatcggc gaccagtacg cagacctttt
4861 tttggcggca aagaacctgt cagacgccat tctgctgagt gatattctgc gagtgaacac
4921 ggagatcacc aaagctccgc tgagcgctag tatgatcaag cgctatgatg agcaccacca
4981 agacttgact ttgctgaagg cccttgtcag acagcaactg cctgagaagt acaaggaaat
5041 tttcttcgat cagtctaaaa atggctacgc cggatacatt gacggcggag caagccagga
5101 ggaattttac aaatttatta agcccatctt ggaaaaaatg gacggcaccg aggagctgct
5161 ggtaaagctt aacagagaag atctgttgcg caaacagcgc actttcgaca atggaagcat
5221 cccccaccag attcacctgg gcgaactgca cgctatcctc aggcggcaag aggatttcta
5281 cccctttttg aaagataaca gggaaaagat tgagaaaatc ctcacatttc ggatacccta
5341 ctatgtaggc cccctcgccc ggggaaattc cagattcgcg tggatgactc gcaaatcaga
5401 agagaccatc actccctgga acttcgagga agtcgtggat aagggggcct ctgcccagtc
5461 cttcatcgaa aggatgacta actttgataa aaatctgcct aacgaaaagg tgcttcctaa
5521 acactctctg ctgtacgagt acttcacagt ttataacgag ctcaccaagg tcaaatacgt
5581 cacagaaggg atgagaaagc cagcattcct gtctggagag cagaagaaag ctatcgtgga
5641 cctcctcttc aagacgaacc ggaaagttac cgtgaaacag ctcaaagaag actatttcaa
5701 aaagattgaa tgtttcgact ctgttgaaat cagcggagtg gaggatcgct tcaacgcatc
5761 cctgggaacg tatcacgatc tcctgaaaat cattaaagac aaggacttcc tggacaatga
5821 ggagaacgag gacattcttg aggacattgt cctcaccctt acgttgtttg aagataggga
5881 gatgattgaa gaacgcttga aaacttacgc tcatctcttc gacgacaaag tcatgaaaca
5941 gctcaagagg cgccgatata caggatgggg gcggctgtca agaaaactga tcaatgggat
6001 ccgagacaag cagagtggaa agacaatcct ggattttctt aagtccgatg gatttgccaa
6061 ccggaacttc atgcagttga tccatgatga ctctctcacc tttaaggagg acatccagaa
6121 agcacaagtt tctggccagg gggacagtct tcacgagcac atcgctaatc ttgcaggtag
6181 cccagctatc aaaaagggaa tactgcagac cgttaaggtc gtggatgaac tcgtcaaagt
6241 aatgggaagg cataagcccg agaatatcgt tatcgagatg gcccgagaga accaaactac
6301 ccagaaggga cagaagaaca gtagggaaag gatgaagagg attgaagagg gtataaaaga
6361 actggggtcc caaatcctta aggaacaccc agttgaaaac acccagcttc agaatgagaa
6421 gctctacctg tactacctgc agaacggcag ggacatgtac gtggatcagg aactggacat
6481 caatcggctc tccgactacg acgtggatca tatcgtgccc cagtcttttc tcaaagatga
6541 ttctattgat aataaagtgt tgacaagatc cgataaaaat agagggaaga gtgataacgt
6601 cccctcagaa gaagttgtca agaaaatgaa aaattattgg cggcagctgc tgaacgccaa
6661 actgatcaca caacggaagt tcgataatct gactaaggct gaacgaggtg gcctgtctga
6721 gttggataaa gccggcttca tcaaaaggca gcttgttgag acacgccaga tcaccaagca
6781 cgtggcccaa attctcgatt cacgcatgaa caccaagtac gatgaaaatg acaaactgat
6841 tcgagaggtg aaagttatta ctctgaagtc taagctggtc tcagatttca gaaaggactt
6901 tcagttttat aaggtgagag agatcaacaa ttaccaccat gcgcatgatg cctacctgaa
6961 tgcagtggta ggcactgcac ttatcaaaaa atatcccaag cttgaatctg aatttgttta
7021 cggagactat aaagtgtacg atgttaggaa aatgatcgca aagtctgagc aggaaatagg
7081 caaggccacc gctaagtact tcttttacag caatattatg aattttttca agaccgagat
7141 tacactggcc aatggagaga ttcggaagcg accacttatc gaaacaaacg gagaaacagg
7201 agaaatcgtg tgggacaagg gtagggattt cgcgacagtc cggaaggtcc tgtccatgcc
7261 gcaggtgaac atcgttaaaa agaccgaagt acagaccgga ggcttctcca aggaaagtat
7321 cctcccgaaa aggaacagcg acaagctgat cgcacgcaaa aaagattggg accccaagaa
7381 atacggcgga ttcgattctc ctacagtcgc ttacagtgta ctggttgtgg ccaaagtgga
7441 gaaagggaag tctaaaaaac tcaaaagcgt caaggaactg ctgggcatca caatcatgga
7501 gcgatcaagc ttcgaaaaaa accccatcga ctttctcgag gcgaaaggat ataaagaggt
7561 caaaaaagac ctcatcatta agcttcccaa gtactctctc tttgagcttg aaaacggccg
7621 gaaacgaatg ctcgctagtg cgggcgagct gcagaaaggt aacgagctgg cactgccctc
7681 taaatacgtt aatttcttgt atctggccag ccactatgaa aagctcaaag ggtctcccga
7741 agataatgag cagaagcagc tgttcgtgga acaacacaaa cactaccttg atgagatcat
7801 cgagcaaata agcgaattct ccaaaagagt gatcctcgcc gacgctaacc tcgataaggt
7861 gctttctgct tacaataagc acagggataa gcccatcagg gagcaggcag aaaacattat
7921 ccacttgttt actctgacca acttgggcgc gcctgcagcc ttcaagtact tcgacaccac
7981 catagacaga aagcggtaca cctctacaaa ggaggtcctg gacgccacac tgattcatca
8041 gtcaattacg gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag
8101 ggctgacccc aagaagaaga ggaaggtgtg atcgatccag gcctcccagc tttcgtccgt
8161 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc ccacacacca
8221 gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct atcatttgtt
8281 ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa tgcaaatgga
8341 tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat tattatctgt
8401 tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt gatattaagg
8461 tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct tgaattggtt
8521 tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa gtaattatgc
8581 aatcaagtta agtacagtat aggctttttg tgtcgagggg gtaccgagtc gaggaattca
8641 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
8701 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
8761 ccttcccaac agttgcgcag cctgaatggc gggtaccgag ctcgaattca attcggcgtt
8821 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
8881 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
8941 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
9001 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
9061 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
9121 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tcgttcggtt
9181 cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg
9241 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat gacatgtttt
9301 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
9361 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
9421 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
9481 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
9541 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
9601 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
9661 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
9721 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
9781 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
9841 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
9901 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
9961 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
10021 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
10081 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
10141 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
10201 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
10261 cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc caaactatca
10321 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga
10381 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
10441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
10501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
10561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
10621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
10681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
10741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
10801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
10861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
10921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
10981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
11041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
11101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
11161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
11221 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
11281 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
11341 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
11401 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
11461 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
11521 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
11581 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
11641 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc
11701 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
11761 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
11821 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
11881 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt
11941 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
12001 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
12061 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
12121 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
12181 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
12241 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
12301 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
12361 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
12421 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
12481 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
12541 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
12601 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
12661 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
12721 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
12781 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
12841 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
12901 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
12961 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
13021 gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc
13081 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
13141 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
13201 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
13261 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
13321 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
13381 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
13441 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
13501 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
13561 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
13621 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
13681 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
13741 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
13801 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
13861 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
13921 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
13981 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
14041 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
14101 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
14161 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
14221 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
14281 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
14341 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
14401 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
14461 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
14521 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
14581 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
14641 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
14701 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
14761 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
14821 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
14881 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
14941 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
15001 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
15061 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
15121 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
15181 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
15241 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
15301 taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat
15361 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
15421 taataaacgc tcttttctct tag
//
pHDE-35S-Cas9-mCherry载体图谱质粒图谱pdf版和pHDE-35S-Cas9-mCherry载体序列质粒序列等相关资料下载
pHDE-35S-Cas9-mCherry质粒载体应用举例
- 上一篇：pCBC-DT1T2(Cm)
- 下一篇：pGreenII 0800-miRNA
