| 出品公司: |
Invitrogen
|
|---|---|
| 载体名称: | pBudCE4.1 |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | CMV or EF-1a |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4595 bp |
| 5' 测序引物及序列: |
CMV Forward: 5'd[TAATACGACTCACTATAGGG]3' EF-1a Forward:5'd[TCAAGCCTCAGACAGTGGTTC]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6XHis； c-myc epitope |
| 载体抗性: | zeocin |
| 筛选标记: | -- |
| 备注: |
克隆宿主菌E.coli TOP10 ；DH5alpha T1 。 该载体能够独立表达两种基因，是双表达载体。表达水平高。 |
| 产品目录号: | V86420 |
| 稳定性: | -- |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pBudCE4.1载体质粒基本信息
pBudCE4.1质粒图谱载体图谱和pBudCE4.1载体序列质粒序列多克隆位点信息
pBudCE4.1质粒载体简介
pBudCE4.1 载体使用时的注意事项：
现在市面上出售的pBudCE4.1载体，在多克隆位点位置都有突变，我们保证我们载体质粒序列的正确性，请大家放心购买！
1.在使用E.coli 克隆菌株时最好使用不含Tn5基因的菌株，如TOP10和DH5α T1；
2.采用电转法时最好用TOP10；
3.Zeocin 工作浓度推荐25 mg/L ~ 50 mg/L
pBudCE4.1质粒序列载体序列
LOCUS pBudCE4.1 4595 bp DNA SYN
DEFINITION pBudCE4.1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4595
/organism="pBudCE4.1"
/mol_type="other DNA"
promoter 11..627
/label="CMV_immearly_promoter"
misc_feature 90..377
/label="CAG_enhancer"
misc_feature 544..564
/label="CMV_fwd_primer"
promoter 638..656
/label="T7_promoter"
misc_feature complement(823..842)
/label="EBV_rev_primer"
misc_feature complement(1063..1437)
/label="sh_ble"
gene complement(1066..1437)
/label="bleo"
/gene="bleo"
promoter complement(1438..1505)
/label="EM7_promoter"
misc_feature complement(1619..1638)
/label="SV40pro_F_primer"
rep_origin complement(1623..1700)
/label="SV40_origin"
misc_feature 1670..1861
/label="SV40_enhancer"
CDS complement(1877..2791)
/label="ORF frame 2"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
promoter 1884..3051
/label="EF1a_promoter"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
misc_feature 2999..3019
/label="EF1a_fwd_primer"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
misc_feature complement(3218..3235)
/label="BGH_rev_primer"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
terminator 3221..3448
/label="bGH_PA_terminator"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
rep_origin complement(3565..4184)
/label="pBR322_origin"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
misc_feature complement(4315..4337)
/label="pGEX_3_primer"
/translation="MKRRLRTERPFSFVWVTHPPALPSAASSILSPLEQGREAAIFPL
TQLVPTGPALPPRAGRYTAARGQAPEQAGQLETTPVRFSVAALAGPASPNMCAGTHGP
RRRPRAQKPKYQCADPGPHLQDYLARKKASQQVIKNFKWLETYRKQRDRREGATRFAR
GGPSAQARPQLKHEAKGLLKRKARPWLQLGIKNHVLEPGGSNSRHARAITRKEARPAG
TTHGTYLCSGGKPVAKKNVHGDYCTYIRFSPTLGKKAEPVHDITFPVYPAPPSLGTGS
IADPSPQLLGDCGRCALCPLTGTGASRS*"
ORIGIN
1 GCGCGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG
61 TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT
121 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC
181 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGACTATTT ACGGTAAACT GCCCACTTGG
241 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT
301 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA
361 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC
421 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA
481 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT
541 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC
601 TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA
661 CCCAAGCTTG CATTCCTGCA GGTCGACATC GATCTTAAGC AGTACTTCTA GAGGATCCGA
721 ACAAAAACTC ATCTCAGAAG AGGATCTGAA TATGCATACC GGTCATCATC ACCATCACCA
781 TTGAGTTTGA TCCCCGGGAA TTCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
841 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
901 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTGGGGTGG GCGAAGAACT CCAGCATGAG
961 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT
1021 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTAGCACG TGTCAGTCCT GCTCCTCGGC
1081 CACGAAGTGC ACGCAGTTGC CGGCCGGGTC GCGCAGGGCG AACTCCCGCC CCCACGGCTG
1141 CTCGCCGATC TCGGTCATGG CCGGCCCGGA GGCGTCCCGG AAGTTCGTGG ACACGACCTC
1201 CGACCACTCG GCGTACAGCT CGTCCAGGCC GCGCACCCAC ACCCAGGCCA GGGTGTTGTC
1261 CGGCACCACC TGGTCCTGGA CCGCGCTGAT GAACAGGGTC ACGTCGTCCC GGACCACACC
1321 GGCGAAGTCG TCCTCCACGA AGTCCCGGGA GAACCCGAGC CGGTCGGTCC AGAACTCGAC
1381 CGCTCCGGCG ACGTCGCGCG CGGTGAGCAC CGGAACGGCA CTGGTCAACT TGGCCATGGT
1441 TTAGTTCCTC ACCTTGTCGT ATTATACTAT GCCGATATAC TATGCCGATG ATTAATTGTC
1501 AACACGTGCT GATCAGATCC GAAAATGGAT ATACAAGCTC CCGGGAGCTT TTTGCAAAAG
1561 CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC AGAGGCGGCC
1621 TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG
1681 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG
1741 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT
1801 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT
1861 CCACACCCTC GTCGAGCTAG CTTCGTGAGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC
1921 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG
1981 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA
2041 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG
2101 GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC
2161 GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTCCAGTACG TGATTCTTGA
2221 TCCCGAGCTG GAGCCAGGGG CGGGCCTTGC GCTTTAGGAG CCCCTTCGCC TCGTGCTTGA
2281 GTTGAGGCCT GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC
2341 TGTCTCGCTG CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG
2401 CTTTTTTTCT GGCAAGATAG TCTTGTAAAT GCGGGCCAGG ATCTGCACAC TGGTATTTCG
2461 GTTTTTGGGC CCGCGGCCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG
2521 CGGGGCCTGC GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT
2581 GCTCTGGTGC CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC
2641 CGGTCGGCAC CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TCCAGGGGGC
2701 TCAAAATGGA GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA
2761 AGGGCCTTTC CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC
2821 AGGCACCTCG ATTAGTTCTG GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG
2881 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG
2941 CACTTGATGT AATTCTCGTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC
3001 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGTGGT
3061 CGCGGCCGCT TCGAAGGTAC CAGCACAGTG GACTCGAGAG ATCTGGCCGG CTGGGCCCGT
3121 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
3181 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG TGGCGGTAAT
3481 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA
3541 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
3601 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
3661 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
3721 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
3781 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
3841 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
3901 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
3961 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
4021 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
4081 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
4141 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
4201 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGACATTAA CCTATAAAAA
4261 TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG
4321 ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA
4381 AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC
4441 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATATGCGGT GTGAAATACC GCACAGATGC
4501 GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA
4561 GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCA
//
pBudCE4.1载体图谱质粒图谱pdf版和pBudCE4.1载体序列质粒序列等相关资料下载
pBudCE4.1质粒载体应用举例
pBudCE4.1-hrGFP
2020-04-03
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