| 质粒名称: | pArk313 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 200257 |
| 存储实验室: | Justin Eyquem |
|
载体骨架基本信息 |
|
| 载体名称: | pArk6 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6088bp |
| 载体类型: | AAV |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Ark313 Rep/Cap |
|
文献引用方法 |
|
| 材料和方法部分: | pArk313 was a gift from Justin Eyquem (Addgene plasmid # 200257 ; http://n2t.net/addgene:200257 ; RRID:Addgene_200257) |
| 参考文献部分: | An evolved AAV variant enables efficient genetic engineering of murine T cells. Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J. Cell. 2023 Jan 19;186(2):446-460.e19. doi: 10.1016/j.cell.2022.12.022. Epub 2023 Jan 12. 10.1016/j.cell.2022.12.022 PubMed 36638795 |
买家导航
pArk313载体质粒基本信息
pArk313质粒图谱载体图谱和pArk313载体序列质粒序列多克隆位点信息
pArk313质粒载体简介
pArk313质粒序列载体序列
LOCUS pArk313 6088 bp DNA circular SYN 25-AUG-2024
DEFINITION Ark313 rep/cap.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6088)
AUTHORS Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY,
Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A,
Havlik LP, Pimentel H, Asokan A, Eyquem J
TITLE An evolved AAV variant enables efficient genetic engineering of
murine T cells.
JOURNAL Cell. 2023 Jan 19;186(2):446-460.e19. doi:
10.1016/j.cell.2022.12.022. Epub 2023 Jan 12.
PUBMED 36638795
REFERENCE 2 (bases 1 to 6088)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cell.2022.12.022"; journalName: "Cell"; date:
"2023-01-19- 19"; volume: "186"; issue: "2"; pages: "446-460.e19"
FEATURES Location/Qualifiers
source 1..6088
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 4077..4088
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
rep_origin complement(4339..4927)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4419..4438)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5098..5958)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5721..5740
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5959..6063)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gatatcctta aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
61 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
121 gaggtttgaa cgcgcagcca ccacggcggg gttttacgag attgtgatta aggtccccag
181 cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa
241 ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct
301 gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc
361 cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt
421 gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg
481 cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc
541 ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat
601 ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga
661 acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct
721 gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc
781 gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga
841 caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt
901 caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat
961 tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat
1021 ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc
1081 ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt
1141 tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt
1201 ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat
1261 ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat
1321 tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc
1381 gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac
1441 gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg
1501 tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc
1561 aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa
1621 aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc
1681 gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa
1741 atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat
1801 gaatcagaat tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc
1861 cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat
1921 tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtgga
1981 tttggatgac tgcatctttg aacaataaat gatttaaatc aggtatggct gccgatggtt
2041 atcttccaga ttggctcgag gacaacctct ctgagggcat tcgcgagtgg tgggacttga
2101 aacctggagc cccgaaaccc aaagccaacc agcaaaagca ggacgacggc cggggtctgg
2161 tgcttcctgg ctacaagtac ctcggaccct tcaacggact cgacaagggg gagcccgtca
2221 acgcggcgga tgcagcggcc ctcgagcacg acaaggccta cgaccagcag ctcaaagcgg
2281 gtgacaatcc gtacctgcgg tataaccacg ccgacgccga gtttcaggag cgtctgcaag
2341 aagatacgtc ttttgggggc aacctcgggc gagcagtctt ccaggccaag aagagggttc
2401 tcgaaccttt tggtctggtt gaggaaggtg ctaagacggc tcctggaaag aaacgtccgg
2461 tagagcagtc gccacaagag ccagactcct cctcgggcat tggcaagaca ggccagcagc
2521 ccgctaaaaa gagactcaat tttggtcaga ctggcgactc agagtcagtc cccgacccac
2581 aacctctcgg agaacctcca gcaacccccg ctgctgtggg acctactaca atggcttcag
2641 gcggtggcgc accaatggca gacaataacg aaggcgccga cggagtgggt aatgcctcag
2701 gaaattggca ttgcgattcc acatggctgg gcgacagagt catcaccacc agcacccgaa
2761 catgggcctt gcccacctat aacaaccacc tctacaagca aatctccagt gcttcaacgg
2821 gggccagcaa cgacaaccac tacttcggct acagcacccc ctgggggtat tttgatttca
2881 acagattcca ctgccatttc tcaccacgtg actggcagcg actcatcaac aacaattggg
2941 gattccggcc caagagactc aacttcaagc tcttcaacat ccaagtcaag gaggtcacga
3001 cgaatgatgg cgtcacgacc atcgctaata accttaccag cacggttcaa gtcttctcgg
3061 actcggagta ccagttgccg tacgtcctcg gctctgcgca ccagggctgc ctccctccgt
3121 tcccggcgga cgtgttcatg attccgcagt acggctacct aacgctcaac aatggcagcc
3181 aggcagtggg acggtcatcc ttttactgcc tggaatattt cccatcgcag atgctgagaa
3241 cgggcaataa ctttaccttc agctacacct tcgaggacgt gcctttccac agcagctacg
3301 cgcacagcca gagcctggac cggctgatga atcctctcat cgaccagtac ctgtattacc
3361 tgaacagaac tcagaatcag tccgtggtca acccggccga gggcttgctg tttagccggg
3421 ggtctccagc tggcatgtct gttcagccca aaaactggct acctggaccc tgttaccggc
3481 agcagcgcgt ttctaaaaca aaaacagaca acaacaacag caactttacc tggactggtg
3541 cttcaaaata taaccttaat gggcgtgaat ctataatcaa ccctggcact gctatggcct
3601 cacacaaaga cgacaaagac aagttctttc ccatgagcgg tgtcatgatt tttggaaagg
3661 agagcgccgg agcttcaaac actgcattgg acaatgtcat gatcacagac gaagaggaaa
3721 tcaaagccac taaccccgtg gccaccgaaa gatttgggac tgtggcagtc aatctccaga
3781 gcagcagcac agaccctgcg accggagatg tgcatgttat gggagcctta cctggaatgg
3841 tgtggcaaga cagagacgta tacctgcagg gtcctatttg ggccaaaatt cctcacacgg
3901 atggacactt tcacccgtct cctctcatgg gcggctttgg actgaagcac ccgcctcctc
3961 agatcctcat caaaaacacg cctgttcctg cgaatcctcc ggcagagttt tcggctacaa
4021 agtttgcttc attcatcacc cagtattcca caggacaagt gagcgtggag attgaatggg
4081 agctgcagaa agaaaacagc aaacgctgga atcccgaagt gcagtataca tctaactatg
4141 caaaatctgc caacgttgat ttcactgtgg acaacaatgg actttatact gagcctcgcc
4201 ccattggcac ccgttacctc acccgtcccc tgtaattgtc tgttaatcaa taaaccggtt
4261 gattcgtttc agttgaactt tggtctagag atatcagcaa aaggccagga accgtaaaaa
4321 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
4381 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
4501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
4561 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
4621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
4681 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
4741 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
4801 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
4921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
4981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
5101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
5161 tgcctggctc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
5221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
5281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
5341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
5401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
5461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
5521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
5581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
5641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
5701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
5761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
5821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
5881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
5941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6061 gcgcacattt ccccgaaaag tgccacct
//
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pArk313质粒载体应用举例
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