| 质粒名称: | pLV-EF1a-mCherry-MinD-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 213112 |
| 存储实验室: | Scott Coyle |
|
载体骨架基本信息 |
|
| 载体名称: | pLV-EF1a |
| 载体出品公司: | Clontech |
| 空载体大小: | 8699bp |
| 完整质粒大小: | 10253bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cherry-MinD |
| 物种来源: | Escherichia coli |
| 插入大小: | 1554bp |
| 启动子: | EF1a |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | EF1a_F (TCAAGCCTCAGACAGTGGTTC) |
| 3’测序引物 | EF1a_R (TAGAGGGAAACCGTTGCTAGC) |
|
文献引用方法 |
|
| 材料和方法部分: |
pLV-EF1a-mCherry-MinD-IRES-Puro was a gift from Scott Coyle (Addgene plasmid # 213112 ; http://n2t.net/addgene:213112 ; RRID:Addgene_213112)
|
| 参考文献部分: | A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design. Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM. Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007. 10.1016/j.cell.2023.12.007 PubMed 38181787 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLV-EF1a-mCherry-MinD-IRES-Puro 10253 bp ds-DNA circular SYN 26-JAN-2024
DEFINITION Expression of mCherry-MinD in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pLV-EF1a-mCherry-MinD-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10253)
AUTHORS Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
TITLE A programmable reaction-diffusion system for spatiotemporal cell
signaling circuit design.
JOURNAL Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi:
10.1016/j.cell.2023.12.007.
PUBMED 38181787
REFERENCE 2 (bases 1 to 10253)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 26, 2024 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10253
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 106..739
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 786..911
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1408..1641
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1826..1870
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2133..2248
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2326..2537
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
intron 2538..3476
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3433..3453
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3492..3501
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3498..4205
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3643..3661)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3911..3930
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(5296..5313)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5523..5542
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5704..6303
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5704..5723)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6200..6220
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6327..6915
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6380..6400)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6798..6809)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7122..7755
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7883..7899)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7883..7899)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7896..7918)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7907..7923
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7931..7961)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7976..7997
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8114..8131)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8285..8873)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8365..8384)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9044..9904)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9667..9686
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9905..10009)
/gene="bla"
/label=AmpR promoter
polyA_signal 10057..10191
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10094..10113)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10148..10167
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
61 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
121 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
181 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
241 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
301 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
361 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
421 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
481 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
541 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
1021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
1081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
1141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
1201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
1261 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
1321 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
1381 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
1441 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
1501 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
1561 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
1621 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
1681 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
1741 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
1801 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
1861 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
1921 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
1981 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
2041 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
2101 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
2161 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
2221 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
2281 tttatcgatg aggccctttc gtcttcactc gaggtgcccg tcagtgggca gagcgcacat
2341 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
2401 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
2461 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
2521 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
2581 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
2641 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
2701 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
2761 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
2821 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
2881 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
2941 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
3001 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
3061 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
3121 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
3181 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
3241 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
3301 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
3361 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
3421 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
3481 cgtgaggatc cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
3541 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
3601 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
3661 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
3721 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
3781 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
3841 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
3901 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
3961 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
4021 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
4081 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
4141 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
4201 acaaggacag cgccggaagt gctggctctg cgggtaccat ggcccgcata atagttgtga
4261 cttcaggtaa gggcggagtt ggaaaaacca catccagcgc agccatagct accggcctcg
4321 cacagaaagg caagaaaact gtggtcatag attttgatat tgggctcagg aacctggacc
4381 tgataatggg ctgcgaacgg cgcgttgtct acgattttgt caatgtcatc caaggcgacg
4441 cgactcttaa tcaggcactt ataaaagata aacgaaccga aaacttgtat attcttcctg
4501 cctcacaaac gcgggacaaa gacgcgctca ccagggaggg tgtcgcaaaa gttttggatg
4561 acttgaaagc gatggacttt gagttcatag tttgtgactc tcccgccggt atagagacag
4621 gggcacttat ggcactgtac ttcgcagatg aagccataat cacaactaat ccggaggtgt
4681 ctagtgtgcg agattcagac cgaatactgg ggatactcgc cagcaaatca cggagggcag
4741 aaaacgggga ggagccaatt aaggagcatc tcctgcttac ccgatataac cccggtcgag
4801 tatctcgggg agatatgctt tcaatggaag atgtcctcga gattctgagg ataaaactgg
4861 taggcgttat cccggaagac cagtccgtcc tccgcgcatc taatcagggg gagcctgtca
4921 tacttgacat aaatgcggat gctgggaaag cttatgccga tacggtagaa cggctcctcg
4981 gggaagaacg gcccttccgc ttcatagagg aggaaaagaa gggattcctt aaacggctgt
5041 tcgggggata agcggccgct ctagagtcga cgggccgcgg taacaattgt taactaactt
5101 aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
5161 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
5221 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
5281 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
5341 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
5401 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
5461 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
5521 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
5581 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
5641 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
5701 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
5761 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
5821 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
5881 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
5941 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
6001 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
6061 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
6121 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
6181 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
6241 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
6301 tgacccgggc ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
6361 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
6421 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
6481 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
6541 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
6601 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
6661 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
6721 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
6781 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
6841 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
6901 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
6961 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
7021 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
7081 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
7141 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
7201 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
7261 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
7321 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
7381 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
7441 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
7501 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
7561 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
7621 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
7681 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
7741 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
7801 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
7861 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
7921 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
7981 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
8041 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
8101 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
8161 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
8221 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
8281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
8341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
8401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
8461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
8521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
8581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
8641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
8701 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
8761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
8821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
8881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
8941 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
9001 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
9061 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
9121 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
9181 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
9241 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
9301 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
9361 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
9421 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
9481 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
9541 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
9601 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
9661 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
9721 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
9781 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
9841 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
9901 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
9961 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
10021 gaaaagtgcc acctgacgtc gacggatcga gagatcaact tgtttattgc agcttataat
10081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
10141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
10201 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cac
//
