| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141389 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 9204bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-orf9b |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141392 ; http://n2t.net/addgene:141392 ; RRID:Addgene_141392) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro 9204 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9204)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9204)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9204
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 1207..1216
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1522..1545
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1582..1605
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 1617..2190
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1784..1801)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2011..2030
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2223..2822
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2223..2242)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2719..2739
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 2836..3424
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2889..2909)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3307..3318)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3631..4264
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4392..4408)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4392..4408)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4405..4427)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4416..4432
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4440..4470)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4485..4506
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4623..4640)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4794..5382)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4874..4893)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5553..6413)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6176..6195
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6414..6518)
/gene="bla"
/label=AmpR promoter
polyA_signal 6566..6700
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6603..6622)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6657..6676
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 6868..7501
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7548..7673
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8170..8403
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8588..8632
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8895..9010
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatggaccc caaaatttct gaaatgcacc cggcgcttcg cttggtggac
1261 ccccaaatcc aactcgctgt cacgcggatg gaaaacgcag taggacgcga ccaaaataat
1321 gtcggcccaa aagtgtatcc gattatcctc cgccttggca gtcccctgag cctgaatatg
1381 gccagaaaga ctcttaacag cctggaggac aaagcgtttc agctgacccc cattgcggtt
1441 cagatgacta agctggcgac aactgaagag ttgcccgacg aatttgtggt agtaaccgta
1501 aagctcgaag gcggcggggg atggagccat ccacaattcg agaaaggcgg tggttcagga
1561 ggaggtagcg ggggtggatc atggtcacat ccgcagtttg aaaagtaagg atcccgcccc
1621 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
1681 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
1741 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
1801 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
1861 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
1921 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
1981 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
2041 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
2101 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
2161 gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt
2221 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
2281 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
2341 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
2401 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
2461 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
2521 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
2581 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
2641 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
2701 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
2761 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
2821 agacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
2881 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
2941 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
3001 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
3061 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
3121 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
3181 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
3241 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
3301 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
3361 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
3421 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
3481 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
3541 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
3601 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
3661 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
3721 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
3781 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
3841 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
3901 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
3961 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
4021 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
4081 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
4141 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
4201 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
4261 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
4321 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
4381 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
4441 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
4501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
4561 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
4621 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4681 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
4741 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4801 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4861 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4921 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4981 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
5041 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
5101 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
5161 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
5221 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
5281 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
5341 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
5401 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
5461 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
5521 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
5581 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
5641 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
5701 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
5761 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
5821 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
5881 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
5941 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
6001 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
6061 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
6121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
6181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
6241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
6301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
6361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
6421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
6481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
6541 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
6601 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
6661 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
6721 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
6781 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
6841 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
6901 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
6961 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
7021 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
7081 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
7141 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
7201 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
7261 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
7321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
7381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
7441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
7501 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
7561 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
7621 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
7681 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
7741 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
7801 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
7861 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
7921 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
7981 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
8041 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
8101 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
8161 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
8221 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
8281 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
8341 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
8401 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
8461 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
8521 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
8581 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
8641 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
8701 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
8761 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
8821 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
8881 tcgacggtat cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
8941 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
9001 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttatcga tgagtaattc
9061 atacaaaagg actcgcccct gccttgggga atcccaggga ccgtcgttaa actcccacta
9121 acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct cgtcatcact
9181 gaggtggaga agagcatgcg tgag
//
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