| 出品公司: | Stratagene |
|---|---|
| 载体名称: | pCMV-Tag 2C |
| 质粒类型: | 哺乳动物表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点；限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 4325 bp |
| 5' 测序引物及序列: | CMV Forward: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | FLAG tag |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | XL1-Blue |
| 宿主细胞（系）: | -- |
| 备注: |
FLAG标签含8个氨基酸残基(DYKDDDDK)； pCMV-Tag 2 A,B,C的差别仅在于读码框（阅读框）处。 |
| 产品目录号: | 211172 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCMV-Tag 2C载体质粒基本信息
pCMV-Tag 2C质粒图谱载体图谱和pCMV-Tag 2C载体序列质粒序列多克隆位点信息
pCMV-Tag 2C质粒载体简介
ADDITIONAL MATERIALS REQUIRED
T4 DNA ligase
Taq DNA polymerase
Taq DNA polymerase buffer
TE buffer
CIAP
Falcon 2059 polypropylene tubes (15-ml)
Water baths (37°C and 42°C)
XL1-Blue Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZΔM15 Tn10 (Tetr)].
The XL1-Blue cells supplied with the pCMV-Tag vectors are not competent cells. Refer to Hanahan (1983) for a protocol for producing competent cells.
pCMV-Tag 2C质粒序列载体序列
LOCUS pCMV-Tag 2C 4325 bp DNA SYN
DEFINITION pCMV-Tag 2C
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4325
/organism="pCMV-Tag 2C"
/mol_type="other DNA"
promoter 16..568
/label="CMV_immearly_promoter"
misc_feature 71..358
/label="CAG_enhancer"
misc_feature 525..545
/label="CMV_fwd_primer"
promoter 526..595
/label="CMV_promoter"
promoter 620..639
/label="T3_promoter"
misc_feature complement(755..771)
/label="pBluescriptKS_primer"
promoter complement(827..845)
/label="T7_promoter"
misc_feature 902..961
/label="SV40_3_splice"
terminator 1012..1240
/label="SV40_PA_terminator"
misc_feature 1210..1229
/label="EBV_rev_primer"
rep_origin complement(1379..1685)
/label="f1_origin"
promoter 1764..1792
/label="AmpR_promoter"
misc_feature complement(1858..1878)
/label="pBABE_3_primer"
misc_feature complement(1864..2079)
/label="SV40_enhancer"
promoter 1876..2144
/label="SV40_promoter"
rep_origin 2043..2120
/label="SV40_origin"
misc_feature 2105..2124
/label="SV40pro_F_primer"
CDS 2227..3021
/label="ORF frame 1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 2230..3018
/label="NeoR/KanR (variant)"
/gene="NeoR/KanR (variant)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
CDS complement(2536..3072)
/label="ORF frame 3"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
terminator 3196..3465
/label="TK_PA_terminator"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
rep_origin 3613..4232
/label="pBR322_origin"
/translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
PPHCISHDGYFLGRSKVR*"
ORIGIN
1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagaagcc cgggcggatc
721 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
781 cggtacctta attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg
841 tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaga
901 tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
961 ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa
1021 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
1081 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
1141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
1201 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
1261 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
1321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
1381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
1441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
1501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
1561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
1621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
1681 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
1801 taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac cagctgtgga
1861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
1921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
1981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
2041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
2101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
2161 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
2221 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
2281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
2341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
2401 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
2461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
2521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
2581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
2641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
2701 gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
2761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
2821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
2881 caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga atgggctgac
2941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
3001 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
3061 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
3121 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
3181 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
3241 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
3301 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
3361 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
3421 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
3481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
3541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
3601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
3661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
3721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
3781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
3841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
3901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
3961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
4021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
4081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
4141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
4201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
4261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
4321 ccgcc
//
pCMV-Tag 2C载体图谱质粒图谱pdf版和pCMV-Tag 2C载体序列质粒序列等相关资料下载
pCMV-Tag 2C质粒载体应用举例
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