LOCUS 12xMoonTag-12xSunTag-kif18b-24xPP7 11017 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses 12xgp41 peptide array (MoonTag peptide array), followed by
12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24
repeats of PP7 hairpins in 3' UTR..
ACCESSION .
VERSION .
KEYWORDS 12xMoonTag-12xSunTag-kif18b-24xPP7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11017)
AUTHORS Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis
LD, Tanenbaum ME
TITLE Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
in mRNA Decoding.
JOURNAL Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi:
10.1016/j.cell.2019.05.001.
PUBMED 31178119
REFERENCE 2 (bases 1 to 11017)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11017
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 820..838
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 841..859
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 869..893
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 984..993
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1029..1073
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1107..1151
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1185..1229
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1263..1307
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1344..1388
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1425..1469
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1506..1550
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1587..1631
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1668..1712
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1749..1793
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1827..1871
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1908..1952
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 1992..2048
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2064..2120
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2136..2192
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2208..2264
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2280..2336
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2352..2408
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2424..2480
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2496..2552
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2568..2624
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2640..2696
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2712..2768
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 2784..2840
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
primer_bind complement(7025..7042)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7031..7255
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7301..7729
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7388..7407)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7598..7619
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7738..7758)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7743..8072
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7923..8058
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7985..8004
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8120..8167
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8186..8560
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8690..8811
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8727..8746)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8781..8800
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8860..8876)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8860..8876)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8873..8895)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8884..8900
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8908..8938)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8953..8974
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9091..9108)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9262..9850)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9342..9361)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10021..10881)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10644..10663
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10882..10986)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
1021 tcggtccgaa gaacgagcag gaactgctgg agctggacaa atgggcctct ctgggcgggg
1081 gaagcggagg gggctccgga ggaggaaaaa acgaacagga gctgctggag ctggataaat
1141 gggcctccct gggcggaggg tccggaggag ggtccggggg cggaaaaaat gaacaggaac
1201 tgctggagct ggacaagtgg gcaagcctgg gggggggcag cggcggaggc tccggaggcg
1261 gaaaaaatga acaggagctg ctggagctgg ataagtgggc ttctctgggg agcggcgggt
1321 ccggagccgg gagcgctggg tctaagaacg agcaggaact gctggagctg gacaaatggg
1381 cctctctggg cagcggcggg tccggagctg gatctgccgg ctctaaaaac gaacaggagc
1441 tgctggagct ggataaatgg gcctccctgg gctctggcgg cagcggcgcc gggtctgccg
1501 ggtctaaaaa tgaacaggaa ctgctggagc tggacaagtg ggcaagcctg ggagggtctg
1561 cagggggatc cggagcaagc ggaggaaaaa atgaacagga gctgctggag ctggataagt
1621 gggcttctct gggcggatcc gctggggggt ccggagcttc tggcggcaag aacgagcagg
1681 aactgctgga gctggacaaa tgggcctctc tggggggatc cgcaggaggg tctggcgctt
1741 ccggcggcaa aaacgaacag gagctgctgg agctggataa atgggcctcc ctgggaggag
1801 gatctggagg agggtctggg ggaggaaaaa atgaacagga actgctggag ctggacaagt
1861 gggcaagcct gggctctggc ggcagcggcg ccgggtctgc cgggtctaaa aatgaacagg
1921 agctgctgga gctggataag tgggcttctc tgccgcggga ccggtggcgt acgggcacag
1981 aattcagcgg cgaagaatta ctgtcaaaaa attaccatct ggaaaacgaa gtggcccggc
2041 tcaaaaaagg aagcgggtcc ggggaagaac tcttatccaa gaattatcac ttggaaaacg
2101 aggtggctcg cctcaaaaaa ggctccgggt ctggcgaaga actgctcagc aaaaactatc
2161 atctcgagaa cgaggtggca aggctcaaaa aaggaggttc gagcggagaa gagcttctat
2221 ccaagaacta tcacctcgag aacgaggtcg ccagactcaa aaagtctgga agcggttcgg
2281 aagagttgct ttcgaaaaat tatcacctgg aaaacgaggt cgctcgactc aaaaagggtt
2341 caagcggagg cgaagagcta ctcagcaaga attatcatct cgaaaacgaa gtcgcacgtc
2401 tcaaaaaggg ttctggaggg ggggaggaac tgctatcaaa aaactatcac ttggagaacg
2461 aggttgcccg gctcaagaaa ggctctggtt caggagagga actcctgtcg aagaactacc
2521 atttggagaa cgaagttgct cgcctcaaga aaggctctgg ttcaggagag gaattactca
2581 gcaaaaatta ccatctggaa aacgaggttg cacgactcaa gaaaggaggg tccggcggag
2641 aggagcttct ttcgaagaat taccacttgg aaaacgaagt ggcgagactc aagaagggtt
2701 ctggaagtgg ggaggagttg ttatccaaaa actaccacct ggagaacgaa gtcgccaggc
2761 tcaagaagag cggcagcggc agcgaggagc tgctgtccaa gaactaccat ctcgagaacg
2821 aagttgcgcg tctcaagaag catcgagcgc gcaccggtgc agtggaggac agcacgctgc
2881 aagtagtggt acgggtgcgg ccccccaccc ctcgggagct ggacagtcag cggcggccag
2941 tggttcaggt ggtggacgag cgggtgctgg tgtttaaccc tgaggagccc gatggagggt
3001 tccctggcct gaaatggggt ggcacccatg atggccccaa gaagaagggc aaagacctga
3061 cgtttgtctt tgaccgggtc tttggcgagg cggccaccca acaggacgtg ttccagcaca
3121 ccacgcacag cgtcctggac agcttcctcc agggctacaa ctgctcagtg tttgcctacg
3181 gggccaccgg ggctgggaag acacacacca tgctgggaag ggagggggac cccggcatca
3241 tgtacctgac caccgtggaa ctgtacaggc gcctggaggc ccgccagcag gagaagcact
3301 tcgaggtgct catcagctac caggaggtgt ataatgaaca gatccatgac ctcctggagc
3361 ccaaggggcc ccttgccatc cgcgaggacc ccgacaaggg ggtggtggtg caaggacttt
3421 ctttccacca gccagcctca gccgagcagc tgctggagat actgaccagg gggaaccgta
3481 accgcacgca gcaccccact gatgccaacg cgacttcctc ccgctcccat gccatcttcc
3541 agatctttgt gaagcagcag gaccgggttc caggactgac ccaggctgtc caggtggcca
3601 agatgagcct gattgacctg gctggctcag agcgggcatc cagcacccat gcgaaggggg
3661 agcggctgcg ggagggggcc aacatcaacc gctctctgct ggcgctcatc aacgtcctca
3721 atgccttggc cgatgcaaag ggccgcaaga ccgctgtgcc ctacgcggac agcgcactga
3781 cccgcctgct caaagactcc ctcgggggca actgccgcac agtgatgatc gctgccatca
3841 gcccctccag cctgacctac gaggacacgt ataataccct caaatatgcc gaccgggcca
3901 aggagatcag gctctcgctg aagagcaatg tgaccagcct ggactgtcac atcagccagt
3961 atgctaccat ctgccaacag ctccaggctg aggtagccgc tctgaggaag aagctccaag
4021 tgtatgaggg gggaggccag cccccaccac aggacctccc aggatctccc aagtcgggac
4081 caccaccaga acaccttccc agctccccct tgccacccca ccctcccagc cagccctgca
4141 ccccagagct ccctgcaggg cctagagccc ttcaagagga gagtctgggg atggaggccc
4201 aggtggagag ggccatggaa gggaactctt cagaccagga gcagtcccca gaggatgagg
4261 atgaaggccc agctgaggag gttccaaccc agatgccaga gcagaacccc acacatgcac
4321 tgccagagtc ccctcgcctg accctgcagc ccaagccagt cgtgggccac ttctcagcac
4381 gggaactgga tggggaccgt tctaagcggt tggccctaaa ggtgctgtgc gttgcccagc
4441 ggcagtactc cctgctccaa gcagccaacc tcctgacgcc cgacatgatc acagagtttg
4501 agaccctaca gcagctggtg caagaggaaa aaattgagcc tggggcagag gccttgagga
4561 cttcaggcct ggccaggggg gcacctctgg ctcaggagct gtgttcagag tcaaagcctc
4621 caggatacac tggccctgtg acccggacta tggcgaggcg actgagtggc cccctgcaca
4681 ccctgggaat cccgcctgga cccaactgca ccccagccca ggggtcccga tggcccatgg
4741 agaagaagag gaggagacca agcgccttgg aggcagacag tcccatggcc ccaaagcggg
4801 gcaccaagcg ccagcgccag tccttcctgc cctgcctaag gagagggtct ctgcctgaca
4861 cccaaccttc acaggggccc agcaccccca aaggagaaag ggcctcctcc ccctgccatt
4921 cccctcgcgt ttgcccagcc acagtcatca aaagccgggt gcccctgggc ccttccgcca
4981 tgcagaactg ctccaccccg ctggctctgc ccactcgaga cctcaatgcc acctttgatc
5041 tctctgagga gcctccctca aagcccagtt tccatgaatg cattggctgg gacaaaatac
5101 cccaggagct gagcaggctg gaccagccct tcatccccag ggcacctgtg cccctgttca
5161 ccatgaaggg ccccaagcca acatcttccc tccctgggac ctctgcctgc aagaagaagc
5221 gcgttgcgag ttcctcagtc tcccatggcc gcagccgcat cgcccgcctc cccagcagca
5281 ctttgaagag gccagctggg ccccttgtac tcccagagct gcccttgagt cccctgtgcc
5341 ctagcaaccg gaggaatgga aaggacctca tcagggtggg gagagcactc tcagcaggga
5401 acggcgtcac caaggtgtcc gatatctaac gacacgattc gaagatcgca tcgctagccc
5461 ggttgtacag gtaccggatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
5521 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
5581 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
5641 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
5701 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
5761 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
5821 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
5881 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
5941 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
6001 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
6061 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga
6121 tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc
6181 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg
6241 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
6301 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
6361 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
6421 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
6481 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
6541 ggctgcagta ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag
6601 acgatatggc gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc
6661 tggctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca
6721 gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg
6781 ctggctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc
6841 agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc
6901 gctggctgca gtattcccgg gttcattaga tctcgcgaag ggcgaattca cgaggcgcgc
6961 ccggatcgcg atcgcaatct taattaactc gagtctagag ggcccgttta aacccgctga
7021 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
7081 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
7141 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
7201 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
7261 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
7321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
7381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
7441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
7501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
7561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
7621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
7681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
7741 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
7801 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
7861 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
7921 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
7981 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
8041 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
8101 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
8161 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
8221 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
8281 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
8341 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
8401 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
8461 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
8521 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
8581 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
8641 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
8701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
8761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
8821 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
8881 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
8941 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
9001 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
9061 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
9121 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
9181 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
9241 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
9301 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
9361 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
9421 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
9481 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
9541 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
9601 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
9661 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
9721 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
9781 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
9841 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
9901 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
9961 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
10021 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10081 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10141 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10201 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
10261 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
10321 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
10381 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
10441 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
10501 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
10561 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
10621 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
10681 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
10741 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
10801 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
10861 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
10921 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
10981 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
