| 质粒名称: | EGFR WT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 11011 |
| 存储实验室: | Matthew Meyerson |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE puro |
| 载体出品公司: | available at Addgene |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFR,epidermal growth factor receptor,ERBB |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3639 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SnaBI（在克隆过程中被销毁） |
| 3' 克隆位点: | SnaBI（在克隆过程中被销毁） |
| 5' 测序引物 | pBABE5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | EGFR WT was a gift from Matthew Meyerson (Addgene plasmid # 11011 ; http://n2t.net/addgene:11011 ; RRID:Addgene_11011) |
| 参考文献部分: | Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR Mutants. Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M, Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M. PLoS Med. 2005 Oct 4. 2(11):e313. 10.1371/journal.pmed.0020313 PubMed 16187797 |
买家导航
EGFRWT载体基本信息
EGFRWT载体图谱质粒图谱和多克隆位点信息
EGFRWT载体简介
EGFRWT载体序列
LOCUS EGFR WT 8725 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral construct for expressing human EGFR in human cells.
ACCESSION .
VERSION .
KEYWORDS EGFR WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8725)
AUTHORS Greulich H, Chen TH, Feng W, Janne PA, Alvarez JV, Zappaterra M,
Bulmer SE, Frank DA, Hahn WC, Sellers WR, Meyerson M
TITLE Oncogenic Transformation by Inhibitor-Sensitive and -Resistant EGFR
Mutants.
JOURNAL PLoS Med. 2005 Oct 4. 2(11):e313.
PUBMED 16187797
REFERENCE 2 (bases 1 to 8725)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8725
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 15..484
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 549..748
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 949..1365
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1295..1317
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1333..1349
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1393..5025
/codon_start=1
/product="precursor of human epidermal growth factor
receptor"
/label=EGFR
/translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
primer_bind complement(5062..5082)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5067..5396
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5247..5382
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5309..5328
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5406..6005
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5406..5425)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5902..5922
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6226..6695
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6825..6842)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6996..7584)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7076..7095)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7744..8604)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8367..8386
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8605..8709)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtgccacctg cagcctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
61 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
121 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
181 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
241 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
301 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
361 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
421 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
481 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
541 cgggaggcaa gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact
601 gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt
661 ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt
721 gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca
781 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
841 ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt
901 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt
961 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
1021 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
1081 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
1141 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
1201 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
1261 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
1321 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggccg gatcccagtg
1381 tggtggtaca tcatgcgacc ctccgggacg gccggggcag cgctcctggc gctgctggct
1441 gcgctctgcc cggcgagtcg ggctctggag gaaaagaaag tttgccaagg cacgagtaac
1501 aagctcacgc agttgggcac ttttgaagat cattttctca gcctccagag gatgttcaat
1561 aactgtgagg tggtccttgg gaatttggaa attacctatg tgcagaggaa ttatgatctt
1621 tccttcttaa agaccatcca ggaggtggct ggttatgtcc tcattgccct caacacagtg
1681 gagcgaattc ctttggaaaa cctgcagatc atcagaggaa atatgtacta cgaaaattcc
1741 tatgccttag cagtcttatc taactatgat gcaaataaaa ccggactgaa ggagctgccc
1801 atgagaaatt tacaggaaat cctgcatggc gccgtgcggt tcagcaacaa ccctgccctg
1861 tgcaacgtgg agagcatcca gtggcgggac atagtcagca gtgactttct cagcaacatg
1921 tcgatggact tccagaacca cctgggcagc tgccaaaagt gtgatccaag ctgtcccaat
1981 gggagctgct ggggtgcagg agaggagaac tgccagaaac tgaccaaaat catctgtgcc
2041 cagcagtgct ccgggcgctg ccgtggcaag tcccccagtg actgctgcca caaccagtgt
2101 gctgcaggct gcacaggccc ccgggagagc gactgcctgg tctgccgcaa attccgagac
2161 gaagccacgt gcaaggacac ctgcccccca ctcatgctct acaaccccac cacgtaccag
2221 atggatgtga accccgaggg caaatacagc tttggtgcca cctgcgtgaa gaagtgtccc
2281 cgtaattatg tggtgacaga tcacggctcg tgcgtccgag cctgtggggc cgacagctat
2341 gagatggagg aagacggcgt ccgcaagtgt aagaagtgcg aagggccttg ccgcaaagtg
2401 tgtaacggaa taggtattgg tgaatttaaa gactcactct ccataaatgc tacgaatatt
2461 aaacacttca aaaactgcac ctccatcagt ggcgatctcc acatcctgcc ggtggcattt
2521 aggggtgact ccttcacaca tactcctcct ctggatccac aggaactgga tattctgaaa
2581 accgtaaagg aaatcacagg gtttttgctg attcaggctt ggcctgaaaa caggacggac
2641 ctccatgcct ttgagaacct agaaatcata cgcggcagga ccaagcaaca tggtcagttt
2701 tctcttgcag tcgtcagcct gaacataaca tccttgggat tacgctccct caaggagata
2761 agtgatggag atgtgataat ttcaggaaac aaaaatttgt gctatgcaaa tacaataaac
2821 tggaaaaaac tgtttgggac ctccggtcag aaaaccaaaa ttataagcaa cagaggtgaa
2881 aacagctgca aggccacagg ccaggtctgc catgccttgt gctcccccga gggctgctgg
2941 ggcccggagc ccagggactg cgtctcttgc cggaatgtca gccgaggcag ggaatgcgtg
3001 gacaagtgca accttctgga gggtgagcca agggagtttg tggagaactc tgagtgcata
3061 cagtgccacc cagagtgcct gcctcaggcc atgaacatca cctgcacagg acggggacca
3121 gacaactgta tccagtgtgc ccactacatt gacggccccc actgcgtcaa gacctgcccg
3181 gcaggagtca tgggagaaaa caacaccctg gtctggaagt acgcagacgc cggccatgtg
3241 tgccacctgt gccatccaaa ctgcacctac ggatgcactg ggccaggtct tgaaggctgt
3301 ccaacgaatg ggcctaagat cccgtccatc gccactggga tggtgggggc cctcctcttg
3361 ctgctggtgg tggccctggg gatcggcctc ttcatgcgaa ggcgccacat cgttcggaag
3421 cgcacgctgc ggaggctgct gcaggagagg gagcttgtgg agcctcttac acccagtgga
3481 gaagctccca accaagctct cttgaggatc ttgaaggaaa ctgaattcaa aaagatcaaa
3541 gtgctgggct ccggtgcgtt cggcacggtg tataagggac tctggatccc agaaggtgag
3601 aaagttaaaa ttcccgtcgc tatcaaggaa ttaagagaag caacatctcc gaaagccaac
3661 aaggaaatcc tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc
3721 ctgctgggca tctgcctcac ctccaccgtg caactcatca cgcagctcat gcccttcggc
3781 tgcctcctgg actatgtccg ggaacacaaa gacaatattg gctcccagta cctgctcaac
3841 tggtgtgtgc agatcgcaaa gggcatgaac tacttggagg accgtcgctt ggtgcaccgc
3901 gacctggcag ccaggaacgt actggtgaaa acaccgcagc atgtcaagat cacagatttt
3961 gggctggcca aactgctggg tgcggaagag aaagaatacc atgcagaagg aggcaaagtg
4021 cctatcaagt ggatggcatt ggaatcaatt ttacacagaa tctataccca ccagagtgat
4081 gtctggagct acggggtgac cgtttgggag ttgatgacct ttggatccaa gccatatgac
4141 ggaatccctg ccagcgagat ctcctccatc ctggagaaag gagaacgcct ccctcagcca
4201 cccatatgta ccatcgatgt ctacatgatc atggtcaagt gctggatgat agacgcagat
4261 agtcgcccaa agttccgtga gttgatcatc gaattctcca aaatggcccg agacccccag
4321 cgctaccttg tcattcaggg ggatgaaaga atgcatttgc caagtcctac agactccaac
4381 ttctaccgtg ccctgatgga tgaagaagac atggacgacg tggtggatgc cgacgagtac
4441 ctcatcccac agcagggctt cttcagcagc ccctccacgt cacggactcc cctcctgagc
4501 tctctgagtg caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa
4561 agctgtccca tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc
4621 ttgactgagg acagcataga cgacaccttc ctcccagtgc ctgaatacat aaaccagtcc
4681 gttcccaaaa ggcccgctgg ctctgtgcag aatcctgtct atcacaatca gcctctgaac
4741 cccgcgccca gcagagaccc acactaccag gacccccaca gcactgcagt gggcaacccc
4801 gagtatctca acactgtcca gcccacctgt gtcaacagca cattcgacag ccctgcccac
4861 tgggcccaga aaggcagcca ccaaattagc ctggacaacc ctgactacca gcaggacttc
4921 tttcccaagg aagccaagcc aaatggcatc tttaagggct ccacagctga aaatgcagaa
4981 tacctaaggg tcgcgccaca aagcagtgaa tttattggag catgagatgt aggaattcgc
5041 cagcacagtg gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
5101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
5161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
5221 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
5281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
5341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
5401 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
5461 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
5521 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
5581 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
5641 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
5701 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
5761 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
5821 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
5881 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
5941 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
6001 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
6061 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
6121 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
6181 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
6241 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
6301 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
6361 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
6421 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
6481 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
6541 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
6601 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
6661 tgagtgattg actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt
6721 tggttttttt tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa
6781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
6841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
6901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
6961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
7021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
7081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
7141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
7201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
7261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
7321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
7381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
7441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
7501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
7561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
7621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
7681 acctagatcc ttttgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga
7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
8701 ggttccgcgc acatttcccc gaaaa
//
