| 质粒名称: | FLAG-mCep290 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27381 |
| 存储实验室: | Joseph Gleeson |
|
载体骨架基本信息 |
|
| 载体名称: | pFLAG-CMV2 |
| 载体出品公司: | Sigma |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | 12257 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cep290 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 7557 bp |
| 基因又名: | centrosomal protein 290 |
| 标签/融合蛋白: | FLAG (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NotI(未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: | FLAG-mCep290 was a gift from Joseph Gleeson (爱迪基因 plasmid # 27381) |
| 参考文献部分: | Mutations in CEP290, which encodes a centrosomal protein, cause pleiotropic forms of Joubert syndrome. Valente EM, Silhavy JL, Brancati F, Barrano G, Krishnaswami SR, Castori M, Lancaster MA, Boltshauser E, Boccone L, Al-Gazali L, Fazzi E, Signorini S, Louie CM, Bellacchio E, Bertini E, Dallapiccola B, Gleeson JG. Nat Genet. 2006 Jun . 38(6):623-5. 10.1038/ng1805 PubMed 16682970 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FLAG-mCep290 12257 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FLAG-mCep290
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12257)
AUTHORS Valente EM, Silhavy JL, Brancati F, Barrano G, Krishnaswami SR,
Castori M, Lancaster MA, Boltshauser E, Boccone L, Al-Gazali L,
Fazzi E, Signorini S, Louie CM, Bellacchio E, Bertini E,
Dallapiccola B, Gleeson JG
TITLE Mutations in CEP290, which encodes a centrosomal protein, cause
pleiotropic forms of Joubert syndrome.
JOURNAL Nat Genet. 2006 Jun . 38(6):623-5.
PUBMED 16682970
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|FLAG-mCep290|
FEATURES Location/Qualifiers
source 1..12257
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 126..148
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 140..157
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 141..157
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
enhancer 318..697
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 698..901
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 852..872
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 932..976
/codon_start=1
/product="leader sequence from mouse preprotrypsin"
/label=preprotrypsin leader
/translation="MSALLILALVGAAVA"
CDS 977..1000
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2789..2800
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 8587..9209
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(8786..8805)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
primer_bind complement(9233..9253)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9238..9567
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9418..9553
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9480..9499
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(9605..9624)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9606..9624)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(9638..9654)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9638..9654)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9651..9673)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9662..9678
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9686..9716)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9731..9752
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9869..9886)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10040..10628)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10120..10139)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10799..11659)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11422..11441
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11660..11764)
/gene="bla"
/label=AmpR promoter
rep_origin 11791..12246
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(11878..11897)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 12088..12109
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
901 tcgtttagtg aaccgtcaga attaattcac catgtctgca cttctgatcc tagctcttgt
961 tggagctgca gttgctgact acaaagacga tgacgacaag ctgcggccgc cagtgggatg
1021 gatatctgca gaattcgccc ttatgccacc taatataaag tggaaagaat taatcaaagt
1081 tgatccagat gacctgccac ggcaagaaga gttagcagat aaattattga tatctttatc
1141 caaggtggaa gtaaatgaac taaaaaatga agaccaagaa aacatgatac atctattcag
1201 aattacccag tctctaatga agatgaaagc ccaggaagta gagctcgctt tggaagaagt
1261 tgaaaaggct ggagaagaac aagcaaaatt tgaaaatcaa ttaaagacaa aagtaatgaa
1321 actggaaaat gaactggaga tggctcagca gtctgcaggg ggacgtgaca ctcggttttt
1381 acgtgacgaa attcgccaac ttgagaagca gctggaacaa aaagatagag aattagagga
1441 tatggaaaaa gaattggata aagaaaagaa ggttaatgaa caattggctc ttcgaaatga
1501 ggaggcagaa aatgaaaaca gcaaattaag aagagagaat gagcagcttc ggcaggacat
1561 tattgactac cagaaacaaa tagattcaca gaaagaatca cttctgtcaa ggagaggaga
1621 agacagtgac taccgatcac agttgtctaa aaagaactat gaacctgttc aatatctgga
1681 cgaaatacag accttaacag aagctaatga gaagattgaa gttcagaacc aagaaatgag
1741 gaaaaacctg gaagagtctg tgcaggagat ggagaagatg actgatgagt acaacaggat
1801 gaaagcgctt gtgcatcagt cggatgctgc catggaccag atcaagaagg agaatgagca
1861 ctatcgcctg caagttcgag agctcacgga tcttctgaag gcgaaggatg aagaggacga
1921 tccagtcatg atggctgtta acgcaaaagt ggaagagtgg aagttaattt tgtcttctaa
1981 agatgatgaa atcattgaat atcagcaaat gctacagagt ctgagaggga aacttaaaaa
2041 tgcccaactt gatgcagaca aaagtaacat catggctctg aaacagggta tccaggagcg
2101 agacagtcaa attaagatgc ttactgagca agtagaacag tatacgaaag aaatggaaaa
2161 aaatacgttt attattgaag atttgaaaaa tgaactccaa aaagacaaag gtacttcaaa
2221 cttttatcag cagactcatt atatgaaaat tcactcaaaa gtacaaattt tagaagagaa
2281 aacaaaagag gccgagagaa tagctgagct ggctgaggct gatgccaggg agaaggacaa
2341 agaattggtt gaggctctga agagattaaa agattatgaa tctggagtat atggcttaga
2401 agatgctgtt atcgaaataa agaattgtaa agcccaaatt aaaataagag atggagagat
2461 ggaagtgttg accaaggaga tcaataagct tgagatgaag atcaatgaca tccttgatga
2521 aaacgaagcc cttagagagc gggctggcct tgaacccaag acaatgattg atttgactga
2581 atttagaaac agcaaacggt taaagcagca acagtacaga gctgagaacc aggttctttt
2641 gaaagagatc gaaagtctag aggaggagcg tcttgacttg aaaagaaaga ttcgtcaaat
2701 ggctcaagaa agaggcaaaa ggaacgcagc ctcaggatta accattgacg acttgaactt
2761 atctgaaacc ttttctcatg aaaataaaat agaaggaaga aaactaaatt ttatgagcct
2821 caataatatg aatgaaacac aatcaaagaa tgagtttctt tcaagagaat tggctgaaaa
2881 ggaaaaagat ttagaaagaa gtaggacagt aattgctaaa ttccagagta aactaaaaga
2941 attagtcgaa gaaaataagc aacttgaaga aggtatgaaa gaaattttgc aagctattaa
3001 ggacatgccg aaggattctg atgtgaaagg aggtgaaaca tctttaatca tcccgagtct
3061 tgaaagactg gttaacgcta tggaatcaaa gaatgcagaa ggaatcttcg atgccagctt
3121 gcatctaaaa gcccaagttg accaacttac aggaaggaat gaagaattaa ggcaagaact
3181 caggcaatct cggaaagagg ctgtaaatta ttcacagcag ttggtaaaag caaacttaaa
3241 gattgaccac cttgagaagg aaactgacct tctacgtcag tctgcaggct ccaatgtagt
3301 atacaaaggc atagacttac ccgatgggat agcaccatcc agtgcctata tcatcaattc
3361 tcagaatgaa tacttaatac atcttttgca ggaactagac aataaagaaa agaaattaaa
3421 acatttagaa gattcccttg aagattataa cagaaagttt gcagtgattc gtcatcaaca
3481 aagtttatta tataaagaat acctaagtga aaaggatatt tggaaaacag actctgagat
3541 gataagagag gagaagagaa aactggaaga tcaagctgag caggatgctg tgaaagtaaa
3601 agagtacaac aatttgctca gtgctcttca gatggattcg aacgagatga agaaaatgct
3661 ctcagaaaac agtaggaaaa tcaccgttct gcaagtgaac gagaagtccc tcatccggca
3721 gtacaccacc ttagtggaga tggagcggca cctgagaaag gaaaacggga aacacaggaa
3781 tgacgtcata gccatggaag ccgaagtcac tgaaaaactt ggaagtttgc aaagattcaa
3841 ggaaatggcc atcttcaaga ttgcagctct tcagaaggtt atagataata gtgtttcttt
3901 gtctgaatta gaactagcca ataaacagta caatgagctg actactaagt acagggacat
3961 cttgcagaaa gataatatgc ttgttcaaag aacaagtaac ttagaacacc tggagtgtga
4021 gaatgcatcg ctaaaagaac agatggaggc tatcagtaaa gagctggaga ttacaaagga
4081 aaaactccat accattgaac aggcctggga acaagaaacg aagttaggaa atgactcaaa
4141 catggataag gctaagaaat caatgacaaa cagtgacatt gtttctattt caaagaaaat
4201 cactgtgttg gagatgaagg aattaaatga aaggcagagg gctgaacact gtcagaaaat
4261 gtatgagcac ttaaggactt cattaaagca aatggaagaa cgtaattttg aattggaaac
4321 caagttcact gagcttacta aaatcaacct ggatgcacaa aaggtggagc agatgttgag
4381 agacgaatta gctgatagtg tgaccaaggc agtaagtgat gctgaccgac agcggattct
4441 agaactagag aagagtgaag tggagctcaa agttgaagtg tccaagctga gagagatttc
4501 agatattgcc aaaagacaag ttgatttttt gaattcgcaa caacagtcca gggaaaagga
4561 agtggaatcc ctcagaacgc agctgctgga cttccaggca caatctgacg aaaaggctct
4621 aattgccaaa ttgcaccaac atgttgtctc tcttcaaatt agtgaggcca ctgccctcgg
4681 taagttagag tcagttacgt ccaaactcca gaagatggaa gcctacaatt tgcgcttaga
4741 acagaaactg gatgaaaaag agcaggcgct ctactatgct cgtttggaag gtagaaacag
4801 agcaaaacac ctgcgccaaa ccattcagtc gcttcgaaga cagttcagtg gagctctacc
4861 cttagcacag caggaaaagt tctccaaaac gatgattcag ttgcaaaatg acaaacttaa
4921 gataatgcaa gaaatgaaga attcgcaaca ggaacacaga aatatggaaa acaaaacact
4981 ggagttggaa ttaaaattaa aaggcttaga agaattgatc agtactttaa aggatgccag
5041 gggagcccag aaggtaatca attggcatgt gaaaatagaa gaacttcgcc tccaagaact
5101 taagctaaat agagaactag tcaagggtaa agaagaaatc aaatatttga ataatatcat
5161 ctctgaatat gagcatacaa tcaacagtct agaggaagaa attgttcagc aaagcaagtt
5221 ccatgaagaa agacagatgg cttgggatca aagagaagtt gagctggaac gccagttaga
5281 catttttgat catcagcaaa atgaaatact cagtgcagca caaaagtttg aagactctac
5341 aggatcaatg ccagacccca gcttgcctct tccaaaccaa cttgaaattg ctctaagaaa
5401 aattaaggag aatattcaag taattcttaa aacacaagca acttgcaagt cactagaaga
5461 gaaactaaaa gaaaaagaat ctgctttacg gttggcagag caaaatattc tgtcaagaga
5521 caaagtaatc aatgaactga ggcttcgatt gcctgccacg gctgatcgag aaaaacttat
5581 agctgagcta gaaagaaaag agctggagcc gaaatctcat cacacaatga aaattgccca
5641 ccaaactatt gccaacatgc aggcaaggtt aaatcacaag gaagaagtat tgaagaaata
5701 ccagcacctt ctggagaagg ccagagagga gcaaagagaa attgttaaga agcatgagga
5761 agaccttcat gttcttcatc acaaattaga acaacaggcc gataattcac tcaataaatt
5821 cagacagaca gctcaggatt tacttaagca gtctcctgct ccagttccca ccaacaaaca
5881 tttcattcgt ctggccgaga tggagcagac agtagcagaa caagatgact ctctgtcctc
5941 acttttgacc aaactaaaga aagtatcaaa agatttggaa aaacaaaaag aaatcactga
6001 gttaaaagtc agagagtttg aaaataccaa actacggctc caagaaactc atgccagtga
6061 ggtaaagaaa gtgaaagcag aggtagagga cttaaggcat gctctagccc aagcacacaa
6121 ggactcccag agtttaaagt ctgaactcca ggctcagaaa gaagcaaact ccagagctcc
6181 aacaaccaca atgaggaatc ttgtagacag gctaaagagc caactagcct tgaaagagaa
6241 gcaacaaaag gcacttagtc gagccctgtt ggaacttcgg tcggaaatga cagcagcagc
6301 tgaggaacgt ataatcgctg taacttctca aaaagaggca aatctcaatg ttcaacaagt
6361 tgttgagcgc catactagag agctaaagtc acaaattgaa gatttaaatg aaaatctttt
6421 aaaattgaaa gaagctctta aaacaagtaa gaacaaagaa aattcactag ctgatgattt
6481 aaatgaatta aataatgaac tgcaaaaaaa gcaaaaagct tataataaaa tccttagaga
6541 gaaagatgga attgatcaag aaaatgatga actgagaaga cagattaaaa gactgtccag
6601 tggactgcag agcaaaactt tgatagataa caagcaaagt ttaatcgatg aacttcaaaa
6661 gaaagttaaa aaacttgaaa gccaactgga aagaaaggtg gatgacgtag acataaagcc
6721 ggtgaaggaa aagagtagta aagaagaatt aattaggtgg gaagaaggta agaaatggca
6781 aaccaaagta gagggactac gaaacagact aaaggagaag gaaggagaag cccacggcct
6841 ggcaaagcag ctgaatacct taaaggaact ttttgccaaa gctgataaag agaaacttac
6901 tttgcagaag aaactgaaaa caacaggaat gactgttgac caggttttag gagtgcgagc
6961 tttggaatct gaaaaagagt tggaagagct aaaaaagaaa aatctggacc tagaaaatga
7021 catattatac atgaggaccc agcaggctct tccacgagat tctgttgtgg aagacttaca
7081 tttacaaaat aaataccttc aagaaaaact tcatacttta gaaaaaaaac tttcaaagga
7141 gaaatattct cagtctttga cttcagaaat agagtcagat gatcactgtc aaaaagaaca
7201 agaacttcag aaggaaaatt tgaagttgtc atctgaaaac atcgagctga aatttcaact
7261 tgaacaagca aataaagatt tgccaagact aaagaatcaa gtgaaagatt tgaaggaaat
7321 gtgtgaattt cttaagaaag gaaaactgga acttgagcgg aagcttggtc aggtcagagg
7381 ggctggtaga agtgggaaga caatcccaga actagaaaaa accattgggt taatgaagaa
7441 agtagttgaa aaagtccaaa gagaaaatga acaattgaaa aaggcatcag gaatactgac
7501 tagtgaaaaa atggctacta ttgaggaaga aaatagaaac ttaaaggctg aactagaaaa
7561 gcttaaagct cactttggac gtcagttgag tatgcagttt gaatctaaga acaaaggtac
7621 tgagaaaatt gttgccgaaa atgaacggct tcggaaagaa cttaagaaag aaatagaagc
7681 ctctgagaaa ctgcggatag ctaagaacaa cttagagctg gtgaacgaca agatggcagc
7741 tcaactcgaa gaaactggga agagactaca gtttgcagaa agtagagccc cacagctgga
7801 aggtgctgac agcaagagct ggaagtcaat tgtggtctca agagtgtatg agaccaagat
7861 gaaagagctt gaaagtgaca ttgccaaaaa gaatcaaagt atcactgacc ttaaacagct
7921 tgtaagagaa gcaacagaga gagaacagaa agctaagaaa tacactgaag accttgaaca
7981 acagattgag atcctcaaaa atgttcctga aggtgccgag acagagcaag agcttatacg
8041 ggaactccag cttcttagat tagccaataa tcagatggat aaagaaaggg cagaattaat
8101 ccatcagata gaaattaaca aggaccaaac cagagctgac agtagcatac ctgattctga
8161 tcaactaagg gaaaagataa atgacctgga gacacaactc agaaagttgg agctagaaaa
8221 gcaacattcg aaggaggaag ttaaaaagct gaaaaaagaa ctggaaaatt ttgatccttc
8281 attttttgaa gaaattgaag acctgaagta taattataag gaagaagtga aaaagaatat
8341 cctattagaa gagaagctaa aaaaactgtc ggaacagttt ggatttgaac tgcctagtcc
8401 tcttgctgct tctgaacact cggaagatgg agaaagtcct catagtttcc ctatttatta
8461 gctccctcgt gtaactgcag gtttaaaact attttatgag tgaaaaaagt ttcagctgga
8521 aatcaagcag tctctgtgtt ataagggcga attccagcac actggcggcc gttactagtg
8581 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
8641 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
8701 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
8761 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
8821 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
8881 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
8941 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
9001 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
9061 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
9121 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
9181 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg
9241 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
9301 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
9361 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
9421 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
9481 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
9541 ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt
9601 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa
9661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
9721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
9781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
9841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
9901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
9961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
10021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
10081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
10141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
10201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
10261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
10321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
10381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
10441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
10501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
10561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
10621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
10681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
10741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
10801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
10861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
10921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
10981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
11041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
11101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
11161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
11221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
11281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
11341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
11401 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
11461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
11521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
11581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
11641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
11701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
11761 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
11821 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
11881 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
11941 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
12001 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
12061 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
12121 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
12181 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
12241 caatttccat acaattt
//
