| 质粒名称: | FUW-tetO-lox-hOCT4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20728 |
| 存储实验室: | Rudolf Jaenisch |
|
载体骨架基本信息 |
|
| 载体名称: | FUW |
| 载体出品公司: | Carlos Lois |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 6454 bp |
| 完整质粒大小: | 7537 bp |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | OCT4 |
| 物种来源: | 人源 |
| 插入大小: | 1083 bp |
| 基因又名: | POU5f1 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | LNCX |
|
文献引用方法 |
|
| 材料和方法部分: | FUW-tetO-lox-hOCT4 was a gift from Rudolf Jaenisch (爱迪基因 plasmid # 20728) |
| 参考文献部分: | Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors. Frank Soldner, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W. Bell, Elizabeth G. Cook, Gunnar Hargus, Alexandra Blak, Oliver Cooper, Maisam Mitalipova, Ole Isacson, Rudolf Jaenisch. Cell, Volume 136, Issue 5, 964-977, 6 March 2009 10.1016/j.cell.2009.02.013 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FUW-tetO-lox-hOCT4 7542 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FUW-tetO-lox-hOCT4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7542)
AUTHORS Frank Soldner, Dirk Hockemeyer, Caroline Beard, Qing Gao, George W.
Bell, Elizabeth G. Cook, Gunnar Hargus, Alexandra Blak, Oliver
Cooper, Maisam Mitalipova, Ole Isacson, Rudolf Jaenisch
TITLE Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells
Free of Viral Reprogramming Factors
JOURNAL Cell, Volume 136, Issue 5, 964-977, 6 March 2009
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|FUW-tetO-lox-hOCT4|
FEATURES Location/Qualifiers
source 1..7542
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 6..385
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 387..585
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 540..560
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 603..783
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 830..955
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1448..1681
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2208..2325
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2384..2654
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2687..2724
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2721..2745
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 3931..4519
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3984..4004)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4402..4413)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4522..4538)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4523..4539)
/label=pBluescriptKS
/note="For pBluescript vector"
protein_bind complement(5043..5076)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 5090..5270
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5296..5313)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5302..5413
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(5559..6147)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5639..5658)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6318..7178)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6941..6960
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7179..7283)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7358..7377)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
361 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
421 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
481 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
541 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
601 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
661 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
721 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
781 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
841 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
901 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
961 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
1021 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
1081 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
1141 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
1201 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
1261 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
1321 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
1381 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
1441 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
1501 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
1561 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
1621 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
1681 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
1741 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
1801 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
1861 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
1921 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
1981 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
2041 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
2101 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
2161 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
2221 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
2281 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
2341 gacagcagag atccagtttg gttaatgatc tcgagtttac cactccctat cagtgataga
2401 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
2461 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
2521 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
2581 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
2641 atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgagtag gcgtgtacgg
2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
2821 ttcatggcgg gacacctggc ttcggatttc gccttctcgc cccctccagg tggtggaggt
2881 gatgggccag gggggccgga gccgggctgg gttgatcctc ggacctggct aagcttccaa
2941 ggccctcctg gagggccagg aatcgggccg ggggttgggc caggctctga ggtgtggggg
3001 attcccccat gccccccgcc gtatgagttc tgtgggggga tggcgtactg tgggccccag
3061 gttggagtgg ggctagtgcc ccaaggcggc ttggagacct ctcagcctga gggcgaagca
3121 ggagtcgggg tggagagcaa ctccgatggg gcctccccgg agccctgcac cgtcacccct
3181 ggtgccgtga agctggagaa ggagaagctg gagcaaaacc cggaggagtc ccaggacatc
3241 aaagctctgc agaaagaact cgagcaattt gccaagctcc tgaagcagaa gaggatcacc
3301 ctgggatata cacaggccga tgtggggctc accctggggg ttctatttgg gaaggtattc
3361 agccaaacga ccatctgccg ctttgaggct ctgcagctta gcttcaagaa catgtgtaag
3421 ctgcggccct tgctgcagaa gtgggtggag gaagctgaca acaatgaaaa tcttcaggag
3481 atatgcaaag cagaaaccct cgtgcaggcc cgaaagagaa agcgaaccag tatcgagaac
3541 cgagtgagag gcaacctgga gaatttgttc ctgcagtgcc cgaaacccac actgcagcag
3601 atcagccaca tcgcccagca gcttgggctc gagaaggatg tggtccgagt gtggttctgt
3661 aaccggcgcc agaagggcaa gcgatcaagc agcgactatg cacaacgaga ggattttgag
3721 gctgctgggt ctcctttctc agggggacca gtgtcctttc ctctggcccc agggccccat
3781 tttggtaccc caggctatgg gagccctcac ttcactgcac tgtactcctc ggtccctttc
3841 cctgaggggg aagcctttcc ccctgtctcc gtcaccactc tgggctctcc catgcattca
3901 aactgagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
3961 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
4021 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
4081 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
4141 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
4201 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
4261 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
4321 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
4381 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
4441 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
4501 ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
4561 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
4621 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
4681 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
4741 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
4801 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
4861 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
4921 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
4981 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactcg
5041 agataacttc gtataatgta tgctatacga agttattccg gactgtactg ggtctctctg
5101 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
5161 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
5221 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
5281 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
5341 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
5401 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
5461 aggacagcaa gggggaggat tgggaagaca atagcaggca tgtgagcaaa aggccagcaa
5521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
5581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
5641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
5701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
5761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
5821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
5881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
5941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
6001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
6061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
6121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
6181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
6241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
6301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
6361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
6421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
6481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
6541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
6601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
6661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
6721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
6781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
6841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
6901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
6961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
7021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
7081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
7141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
7201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
7261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
7321 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
7381 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
7441 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
7501 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata ta
//
