| 质粒名称: | GW1-pHRed |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31473 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | GW1 |
| 载体出品公司: | British Biotechnology |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5300 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pHRed |
| 物种来源: | 合成 |
| 插入大小: | 710 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI(未破坏) |
| 3' 克隆位点: | NheI (未破坏) |
| 5' 测序引物 | CACAGCAATCGATCCATG |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | GW1-pHRed was a gift from Gary Yellen (Addgene plasmid # 31473) |
| 参考文献部分: | Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor. Tantama M, Hung YP, Yellen G. J Am Chem Soc. 2011 Jul 6. 133(26):10034-7. 10.1021/ja202902d PubMed 21631110 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GW1-pHRed 5981 bp DNA SYN
DEFINITION GW1-pHRed
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5981
/organism="GW1-pHRed"
/mol_type="other DNA"
CDS 189..899
/label="ORF frame 3"
terminator 1024..1155
/label="SV40_PA_terminator"
misc_feature 1112..1131
/label="EBV_rev_primer"
promoter complement(1175..1193)
/label="M13_reverse_primer"
misc_feature complement(1192..1214)
/label="M13_pUC_rev_primer"
promoter complement(1228..1257)
/label="lac_promoter"
rep_origin complement(1566..2185)
/label="pBR322_origin"
gene complement(2340..3200)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2340..3200)
/label="ORF frame 1"
promoter complement(3279..3307)
/label="AmpR_promoter"
misc_feature complement(3466..3488)
/label="pGEX_3_primer"
misc_feature complement(3675..3694)
/label="SV40pro_F_primer"
misc_feature complement(3725..3868)
/label="lacZ_a"
CDS complement(3725..4252)
/label="ORF frame 2"
misc_feature 3839..3861
/label="M13_pUC_fwd_primer"
promoter 3854..3870
/label="M13_forward20_primer"
promoter 4420..4996
/label="CMV_immearly_promoter"
misc_feature 4499..4786
/label="CAG_enhancer"
misc_feature 4953..4973
/label="CMV_fwd_primer"
promoter 4954..5023
/label="CMV_promoter"
promoter 4966..5085
/label="CMV2_promoter"
misc_feature 4997..5016
/label="pCEP_fwd_primer"
misc_feature 4999..5023
/label="LNCX_primer"
CDS complement(5491..5949)
/label="ORF frame 3"
CDS complement(5852..920)
/label="ORF frame 1"
ORIGIN
1 AAGCTTCCCG GCCACAGCAA TCGATCCATG ACACAACCAG TGACAAGCAG TCCCCCAGTG
61 AAACCGCATC CGCATCCGAG TCCGATACCG ATAAAGATTC TGAATCGGAG TGAGTGCCGC
121 GTCCGAGAGC GTTCCCTGTC CACGTCCACC ATCGGCGGAG CAGGTGTGCC TGAGGCCTCT
181 AGACCACCAT GGTTTCCGTT ATCGCAAAGC AAATGACCTA TAAAGTTTAT ATGTCTGGCA
241 CCGTTAACGG CCACTACTTC GAGGTTGAAG GTGACGGCAA GGGCAAGCCT TACGAGGGCG
301 AGCAGACCGT TAAGCTGACC GTTACCAAAG GCGGCCCACT GCCTTTCGCC TGGGATATCC
361 TGTCCCCTCA GCTCCAATAC GGCTCCATCC CTTTCACCAA ATACCCCGAG GACATCCCTG
421 ACTACTTTAA GCAGAGCTTC CCAGAGGGCT ACACCTGGGA GCGCTCTATG AACTTCGAGG
481 ACGGCGCAGT TTGTACCGTT TCCAACGACA GCTCCATCCA GGGTAACTGC TTCATCTACA
541 ACGTTAAGAT TTCCGGCGAG AACTTCCCAC CTAACGGCCC AGTTATGCAG AAGAAAACCC
601 AAGGCTGGGA GCCCAGCACC GAACGCCTGT TTGCCCGCGA CGGTATGCTG ATTGGTAACG
661 ATTATATGGC CCTGAAACTG GAAGGCGGCG GTCACTACCT GTGCGAGTTC AAGAGCACTT
721 ACAAGGCTAA AAAGCCTGTT CGCATGCCTG GCCGCCACGA AATCGACCGC AAGCTGGATG
781 TTACCTCTCA CAACCGCGAC TACACCAGCG TTGAGCAGTG TGAAATCTCT ATCGCCCGCC
841 ACTCTCTGCT GGGGACAGGC AACTCCGCCG ACGGTGGTGG CTCTGGTGGT GCTAGCTGAG
901 TCGACGAATT CATTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA
961 AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT
1021 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
1081 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
1141 TATCATGTCT GGATCCGTAC CGAGCTCGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
1201 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
1261 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
1321 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
1381 GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT
1441 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
1501 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
1561 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
1621 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
1681 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
1741 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT
1801 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
1861 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
1921 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
1981 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
2041 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
2101 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
2161 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
2221 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
2281 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
2341 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
2401 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
2461 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
2521 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
2581 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
2641 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
2701 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
2761 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
2821 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
2881 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
2941 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
3001 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
3061 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
3121 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
3181 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATAAG CGGCCGCGGC CATGCCGGCC
3241 ACTAGTCTCG AGTTATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
3301 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
3361 GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
3421 CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA
3481 GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA
3541 GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA
3601 GATTGTACTG AGAGTGCACC ATATGAAGAC GTCGCCTCCT CACTACTTCT GGAATAGCTC
3661 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC GCGCCATTCG
3721 CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC
3781 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
3841 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCATCTGCA GTGAATAATA AAATGTGTGT
3901 TTGTCCGAAA TACGCGTTTG AGATTTCTGT CCCGACTAAA TTCATGTCGC GCGATAGTGG
3961 TGTTTATCGC CGATAGAGAT GGCGATATTG GAAAAATCGA TATTTGAAAA TATGGCATAT
4021 TGAAAATGTC GCCGATGTGA GTTTCTGTGT AACTGATATC GCCATTTTTC CAAAAGTTGA
4081 TTTTTGGGCA TACGCGATAT CTGGCGATAC GCTTATATCG TTTACGGGGG ATGGCGATAG
4141 ACGCCTTTGG TGACTTGGGC GATTCTGTGT GTCGCAAATA TCGCAGTTTC GATATAGGTG
4201 ACAGACGATA TGAGGCTATA TCGCCGATAG AGGCGACATC AAGCTGGCAC ATGGCCAATG
4261 CATATCGATC TATACATTGA ATCAATATTG GCCATTAGCC ATATTATTCA TTGGTTATAT
4321 AGCATAAATC AATATTGGCT ATTGGCCATT GCATACGTTG TATCCATATC ATAATATGTA
4381 CATTTATATT GGCTCATGTC CAACATTACC GCCATGTTGA CATTGATTAT TGACTAGTTA
4441 TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC
4501 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
4561 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
4621 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
4681 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC
4741 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT
4801 GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC
4861 AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT
4921 TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG
4981 GGAGGTCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC
5041 ACGCTGTTTT GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGCG GCCGGGAACG
5101 GTGCATTGGA ACGCGGATTC CCCGTGCCAA GAGTGACGTA AGTACCGCCT ATAGAGTCTA
5161 TAGGCCCACC CCCTTGGCTT CTTATGCATG CTATACTGTT TTTGGCTTGG GGTCTATACA
5221 CCCCCGCTTC CTCATGTTAT AGGTGATGGT ATAGCTTAGC CTATAGGTGT GGGTTATTGA
5281 CCATTATTGA CCACTCCCCT ATTGGTGACG ATACTTTCCA TTACTAATCC ATAACATGGC
5341 TCTTTGCCAC AACTCTCTTT ATTGGCTATA TGCCAATACA CTGTCCTTCA GAGACTGACA
5401 CGGACTCTGT ATTTTTACAG GATGGGGTCT CATTTATTAT TTACAAATTC ACATATACAA
5461 CACCACCGTC CCCAGTGCCC GCAGTTTTTA TTAAACATAA CGTGGGATCT CCACGCGAAT
5521 CTCGGGTACG TGTTCCGGAC ATGGGCTCTT CTCCGGTAGC GGCGGAGCTT CTACATCCGA
5581 GCCCTGCTCC CATGCCTCCA GCGACTCATG GTCGCTCGGC AGCTCCTTGC TCCTAACAGT
5641 GGAGGCCAGA CTTAGGCACA GCACGATGCC CACCACCACC AGTGTGCCGC ACAAGGCCGT
5701 GGCGGTAGGG TATGTGTCTG AAAATGAGCT CGGGGAGCGG GCTTGCACCG CTGACGCATT
5761 TGGAAGACTT AAGGCAGCGG CAGAAGAAGA TGCAGGCAGC TGAGTTGTTG TGTTCTGATA
5821 AGAGTCAGAG GTAACTCCCG TTGCGGTGCT GTTAACGGTG GAGGGCAGTG TAGTCTGAGC
5881 AGTACTCGTT GCTGCCGCGC GCGCCACCAG ACATAATAGC TGACAGACTA ACAGACTGTT
5941 CCTTTCCATG GGTCTTTTCT GCAGTCACCG TCCTTGACAC G
//
