| 质粒名称: | HA-TAZ |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32839 |
| 存储实验室: | Kunliang Guan |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3-HA |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TAZ,WWTR1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | HA（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | HA-TAZ was a gift from Kunliang Guan (Addgene plasmid # 32839 ; http://n2t.net/addgene:32839 ; RRID:Addgene_32839) |
| 参考文献部分: | TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway. Lei QY, Zhang H, Zhao B, Zha ZY, Bai F, Pei XH, Zhao S, Xiong Y, Guan KL. Mol Cell Biol. 2008 Apr;28(7):2426-36. Epub 2008 Jan 28. 10.1128/MCB.01874-07 PubMed 18227151 |
买家导航
HATAZ载体基本信息
HATAZ载体图谱质粒图谱和多克隆位点信息
HATAZ载体简介
HATAZ载体序列
LOCUS HA-TAZ 6668 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS HA-TAZ
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6668)
AUTHORS Lei QY, Zhang H, Zhao B, Zha ZY, Bai F, Pei XH, Zhao S, Xiong Y,
Guan KL
TITLE TAZ promotes cell proliferation and epithelial-mesenchymal
transition and is inhibited by the hippo pathway.
JOURNAL Mol Cell Biol. 2008 Apr;28(7):2426-36. Epub 2008 Jan 28.
PUBMED 18227151
REFERENCE 2 (bases 1 to 6668)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6668
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(40..60)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 45..374
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 225..360
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 287..306
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 441..1235
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(495..514)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 1105..1124
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 1409..1530
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1446..1465)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1500..1519
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1579..1595)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1579..1595)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1592..1614)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1603..1619
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1627..1657)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1672..1693
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1810..1827)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1981..2569)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2061..2080)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2740..3600)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3363..3382
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3601..3705)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3780..3799)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3971..4350
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4351..4554
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4505..4525
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4599..4618
/label=T7
/note="T7 promoter, forward primer"
promoter 4599..4617
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4640..4666
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 4670..4696
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(5926..5942)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5927..5943)
/label=pBluescriptKS
/note="For pBluescript vector"
promoter complement(5957..5975)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(5958..5975)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5995..6012)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6001..6225
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6271..31
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6358..6377)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6568..6589
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
61 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
421 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
541 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
601 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
841 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
901 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
961 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
1021 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
1081 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
1141 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
1201 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
1261 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
1321 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
1381 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
1441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1501 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
1561 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
1621 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
1681 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
1741 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
1861 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
1921 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
1981 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
2041 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
2101 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
2161 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
2221 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
2281 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
2341 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
2401 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
2461 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
2521 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
2581 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
2641 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
2701 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
2761 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
2821 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
2881 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
2941 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
3001 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
3061 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
3121 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
3181 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
3241 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
3301 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
3361 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
3421 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
3481 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
3541 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
3601 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
3661 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
3721 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
3781 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
3841 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
3901 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
3961 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
4021 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
4081 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
4141 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
4201 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
4261 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
4321 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
4381 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
4441 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
4501 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
4561 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
4621 acccaagctt gccatggcct acccatacga tgttcctgac tatgcgggct atccctatga
4681 cgtcccggac tatgcaggat ccatgaatcc ggcctcggcg ccccctccgc tcccgccgcc
4741 tgggcagcaa gtgatccacg tcacgcagga cctagacaca gacctcgaag ccctcttcaa
4801 ctctgtcatg aatccgaagc ctagctcgtg gcggaagaag atcctgccgg agtctttctt
4861 taaggagcct gattcgggct cgcactcgcg ccagtccagc accgactcgt cgggcggcca
4921 cccggggcct cgactggctg ggggtgccca acatgtccgc tcgcactcgt cgcccgcgtc
4981 cctgcagctg ggcaccggcg cgggtgctgc gggtagcccc gcgcagcagc acgcgcacct
5041 ccgccagcag tcctacgacg tgaccgacga gctgccactg cccccgggct gggagatgac
5101 cttcacggcc actggccaga ggtacttcct caatcacata gaaaaaatca ccacatggca
5161 agaccctagg aaggcgatga atcagcctct gaatcatatg aacctccacc ctgccgtcag
5221 ttccacacca gtgcctcaga ggtccatggc agtatcccag ccaaatctcg tgatgaatca
5281 ccaacaccag cagcagatgg cccccagtac cctgagccag cagaaccacc ccactcagaa
5341 cccacccgca gggctcatga gtatgcccaa tgcgctgacc actcagcagc agcagcagca
5401 gaaactgcgg cttcagagaa tccagatgga gagagaaagg attcgaatgc gccaagagga
5461 gctcatgagg caggaagctg ccctctgtcg acagctcccc atggaagctg agactcttgc
5521 cccagttcag gctgctgtca acccacccac gatgacccca gacatgagat ccatcactaa
5581 taatagctca gatcctttcc tcaatggagg gccatatcat tcgagggagc agagcactga
5641 cagtggcctg gggttagggt gctacagtgt ccccacaact ccggaggact tcctcagcaa
5701 tgtggatgag atggatacag gagaaaacgc aggacaaaca cccatgaaca tcaatcccca
5761 acagacccgt ttccctgatt tccttgactg tcttccagga acaaacgttg acttaggaac
5821 tttggaatct gaagacctga tccccctctt caatgatgta gagtctgctc tgaacaaaag
5881 tgagcccttt ctaacctggc tgtaagaatt cgatatcaag cttatcgata ccgtcgacct
5941 cgaggggggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
6001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
6061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
6121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
6181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
6241 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
6301 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
6361 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
6421 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
6481 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
6541 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
6601 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
6661 taaaaaat
//
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